Gary L. Johanning, PHD, MS, BS

Present Title & Affiliation

Dual/Joint/Adjunct Appointment

Associate Professor, Department of Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, Smithville, TX

Office Address

Department of Veterinary Sciences, Michale E. Keeling Center for Comparative Medicine and Research, The University of Texas MD Anderson Cancer Center
650 Cool Water Drive
Unit Number: 119
Bastrop, TX 78602
Room Number: BRE1.2014
Phone: 512-321-3991
Fax: 512-332-7375
Email: gljohann@mdanderson.org

Education & Training

Degree-Granting Education
1978	University of Missouri, Columbia, MO, PHD, Degree in Biochemistry
1976	University of Missouri, Columbia, MO, MS, Degree in Biochemistry
1973	University of Missouri, Columbia, MO, BS, Chemistry/Biochemistry
Postgraduate Training
1989-1993	Postdoctoral Fellow, Molecular Biology, Case Western Reserve University, Cleveland, OH

Experience/Service

Academic Appointments

Regular Member of the Graduate Faculty, The University of Texas Health Science Center at Houston, Houston, TX, 6/2004-present

Member, The University of Texas Health Science Center at Houston Graduate School of Biomedical Sciences, Houston, TX, 2/2004-present

Associate Professor, Department of Nutrition Sciences, The University of Alabama at Birmingham, Birmingham, AL, 2002-2004

Associate Scientist, Clinical Nutrition Research Center, The University of Alabama at Birmingham, Birmingham, AL, 2000-2004

Associate Scientist, Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL, 1998-2004

Member, Cell Adhesion and Matrix Research Center, Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL, 1996-2004

Associate Member, Cell Adhesion and Matrix Research Center, The University of Alabama at Birmingham, Birmingham, AL, 1996-2004

Associate Scientist, Cell Adhesion and Matrix Research Center, The University of Alabama at Birmingham, Birmingham, AL, 1995-2004

Assistant Professor, Department of Nutrition Science, The University of Alabama at Birmingham, Birmingham, AL, 1993-2002

Postdoctoral Research Associate, Biochemistry, University of Missouri, Columbia, MO, 1986-1989

Assistant Professor, Department of Chemistry, Armstrong Atlantic State University, Savannah, GA, 1981-1986

Postdoctoral Research Associate, Biochemistry, University of Missouri, Columbia, MO, 1978-1981

Administrative Appointments/Responsibilities

Member, Faculty Senate Academic Planning Committee, The University of Texas MD Anderson Cancer Center, Houston, TX, 2007-2010

Member, Faculty Senate Education Committee, The University of Texas MD Anderson Cancer Center, Houston, TX, 2007-2010

Member, Faculty Senate, The University of Texas MD Anderson Cancer Center, Houston, TX, 9/2006-2010

On-site Graduate School of Biomedical Sciences (GSBS) Advisor, Department of Veterinary Sciences, Michale E. Keeling Center for Comparative Medicine and Research, The University of Texas MD Anderson Cancer Center, Bastrop, TX, 2006-present

Other Appointments/Responsibilities

Member, Advisory Committee of the Cancer Prevention and Control Training Program, The University of Alabama at Birmingham, Birmingham, AL, 2001-2004

Member, Grant Review, University of Alabama at Birmingham, Birmingham, AL, 2000-2001

Member, Campaign for The University of Alabama at Birmingham, Birmingham, AL, 1999-2000

Member, Radiation Safety Committee, The University of Alabama at Birmingham, Birmingham, AL, 1998-2004

Member, PHD Committee, The University of Alabama at Birmingham, Birmingham, AL, 1997-2004

Member, Seminar Committee, The University of Alabama at Birmingham, Birmingham, AL, 1997-1998

Member, Strategic Planning Focus Group, The University of Alabama at Birmingham, Birmingham, AL, 1997

Member, Ad Hoc Faculty Evaluation Committee, The University of Alabama at Birmingham, Birmingham, AL, 1995-1996

Member, Research Design Service Course Subcommittee, The University of Alabama at Birmingham, Birmingham, AL, 1995-1996

Chairman, Nutrition Diagnostic Laboratory Quality Assurance Committee, The University of Alabama at Birmingham, Birmingham, AL, 1994-2004

Member, Faculty Affairs Committee, The University of Alabama at Birmingham, Birmingham, AL, 1994-1999

Member, Research Advisory Committee, The University of Alabama at Birmingham, Birmingham, AL, 1994-1996

Member, Cultural Diversity Faculty Focus Group, The University of Alabama at Birmingham, Birmingham, AL, 1994-1995

Institutional Committee Activities

Chair, Department of Veterinary Sciences Subscriptions/Library Journals Committee, 2008-present

Chair, Developed Graduate School of Biomedical Sciences (GSBS) Course entitled "Application of Animal Models to Translational Research", 8/2007

Chair, Non-Tenure Track Progress Review Committee for Virginia Scofield, PHD, 4/2007

Member, Bastrop Facility Strategic Planning Committee for Basic Research & Education Building, 2006-2007

Member, Safety Committee, Department of Veterinary Sciences, Michale E. Keeling Center for Comparative Medicine and Research, The University of Texas MD Anderson Cancer Center, 2004-present

Honors and Awards

1991-1993	Postdoctoral Fellow, National Institutes of Health, Metabolism Training Grant (AM 07319)
1989-1991	Pew Postdoctoral Fellow, Pew Foundation

Professional Memberships

American Association for Cancer Research
Member, 4/2002-present

American Association for the Advancement of Science
Member, 5/1989-present

American Society of Nutrition Science
Full Member, 1/1990-present

Sigma Xi
Member, 7/1987-present

Selected Publications

Abstracts
1.	Wang-Johanning F, Rycaj K, Huang M, Plummer JB, Marks JW, Johanning GL, Rosenblum MG. Anti-HERV-K antibody 6H5 and 6H5/rGel inhibit cell growth and induce apoptosis in breast and ovarian cancer cells. Proc Annu Meet Am Assoc Cancer Res, 4/2007.
2.	Okegbile EO, Jones A, Johanning G, Grubbs C. Chemopreventive effect of antioxidants on SK-OV-3 tumor-forming ovarian epithelial cancer cells. Proc Annu Meet Am Assoc Cancer Res, 4/2007.
3.	Rycaj K, Wang-Johanning F, Johanning G, Lu M. In vivo antitumor effects of HERV-K env antigen on mammary tumors expressing the HERV-K gene. Proc Annu Meet Am Assoc Cancer Res, 4/2007.
4.	Okegbile EO, Johanning G, Grubbs C. Down regulation of nitric oxide release by antioxidants in ovarian cancer cell proliferation. Proc Annu Meet Am Assoc Cancer Res 47 (#4804), 2006.
5.	Huang M, Liu J, Johanning GL, Wang-Johanning F. Stromal-epithelial cell interactions and ovarian tumor progression. Proc Annu Meet Am Assoc Cancer Res 47 (#1678), 2006.
6.	Piyathilake CJ, Henao O, Azrad M, Macalus M, Johanning G, Cornwell P, Partridge E, Heimburger D. Protective effect of MTHFR polymorphism on cervical intrapithelial neoplasia (CIN) is modified by riboflavin status. FASEB J 19:A1499-A1499, 3/2005.
7.	Okegbile E, Johanning G, Grubbs C. Preliminary studies on the regulation of extracellular interlukin-8 by vitamin E during ovarian cancer progression. FASEB J 18:C109-C110 Suppl. S, 5/2004.
8.	Okegbile E, Johanning GL, Grubbs CJ. Preliminary studies on chemoprevention of ovarian cancer progression by vitamin C. Proc Annu Meet Am Assoc Cancer Res 45 (#1385), 2004.
9.	Henao OL, Piyathilake CJ, Waterbor J. Methylenetetrahydrofolate reductase (MTHFR) and methionine synthase (MS) polymorphisms are associated with a decreased risk of cervical dysplasia. FASEB J 17:A373-A373 Part 1, 2003.
10.	Wang-Johanning F, Teng L, Chen DT, Lu DW, Johanning GL. Microarray analysis of differential gene expression profiles in ovarian cancer. Proc Annu Meet Am Assoc Cancer Res 2nd ED 44 (#6009), 2003.
11.	Piyathilake CJ, Cerfolio RJ, Whiteside MA, Meleth S, Johanning GL, Heimburger DC, Grizzle WE. Alterations in global DNA methylation in buccal mucosal cells reflect changes in tissues at risk of developing non-small cell lung cancer. Proc Annu Meet Am Assoc Cancer Res (#5155), 2002.
12.	Piyathilake CJ, Oelschlager DK, Cerfolio RJ. Associations among folate, vitamin C, vitamin B-12, and global DNA methylation in adenocarcinomas of the lung. FASEB J 16:A268-A268 Part 1, 2002.
13.	Piyathilake CJ, Heimburger DC, Meleth S. Gene-nutrient interactions and global DNA methylation in peripheral leukocytes. FASEB J 16:A264-A264 Part 1, 2002.
14.	Wang-Johanning F, Lu D, Epp L, Johanning G. Construction and functional evaluation of a single-chain antibody against HPV16 E6 and comparison with anti-E7 scFv. Proc Annu Meet Am Assoc Cancer Res 42, 2001.
15.	Johanning GL, Lu D, Wang-Johanning F. Modified assays for evaluation of HPV 16 E6 and E7 mRNA expression in cervical tissues. Proc Annu Meet Am Assoc Cancer Res 42, 2001.
16.	Piyathilake CJ, Whiteside MA, Johanning GL, Henao O, Macaluso M, Heimburger DC. Modulation of cancer risk by genetic polymorphism of methylene tetrahydrofolate reductase (MTHFR) and methionine synthase (MS) may depend on the racial distribution of study subjects. FASEB J 15:A618-A618, 2001.
17.	Piyathilake CJ, Henso O, Macaluso M, Frost AR, Johanning GL, Belt WC, Heimburger DC, Niveleau A, Grizzle WE. Race and age-dependent alterations in global methylation of DNA in squamous cell carcinoma of the lung. FASEB J 15:A618-A618, 2001.
18.	Piyathilake CJ, Oelschlager D, Bell WC, Whiteside MA, Johanning GL, Heimburger DC, Grizzle WE. Variations of vitamin concentrations and DNA methylation in squamous cell cancers of the oral cavity, larynx and lung. FASEB J 15:A619-A619, 2001.
19.	Blaylock ML, Powell CS, Johanning GL, Nagy TR. Downregulation of UCP1 is related to a reduction in resting energy expenditure. FASEB J 14:A756-A756, 2000.
20.	Johanning GL, Wilborn TW, Cope MB, Grubbs CJ, Steele VE, Kelloff GJ, Lubet RA. Effect of high doses of retinoids on RAR-b mRNA expression in female mice. Proc Annu Meet Am Assoc Cancer Res 41:54, 2000.
21.	Piyathilake CJ, Johanning GL, Frost AR, Whiteside MA, Manne U, Grizzle WE, Heimburger DC, Niveleau A. Immunohistochemical evaluation of global DNA methylation: comparison with in vitro ratio-labeled methyl incorporation assay. Proc Annu Meet Am Assoc Cancer Res 41:497-498, 2000.
22.	Piyathilake CJ, Johanning GL, Macaluso M, Whiteside MA, Oelschlager DK, Heimburger DC, Grizzle WE. Localized folate and vitamin B-12 deficiency in squamous cell lung cancer is associated with global DNA hypomethylation. FASEB J 14:A473-A473, 2000.
23.	Moore NG, Wang-Johanning F, Johanning GL. Omega-3 polyunsaturated fatty acids decrease PKARIa subunit expression and adherence of MDA-MB-231 human breast cancer cells. Proc Annu Meet Am Assoc Cancer Res 41:54, 2000.
24.	Wang-Johanning F, Johnson MR, Frost AR, Johanning GL, Wang K, Shaw DR, LoBuglio AF. Quantitation of HERV-K10 mRNA expression by real time RT-PCR in human breast cancer cells microdissected by laser capture. Proc Annu Meet Am Assoc Cancer Res 41:315, 2000.
25.	Piyathilake CJ, Bell WC, Johanning GL, Cornwell PE, Heimburger DC, Grizzle WE. The accumulation of ascorbic acid by squamous cell cancers of the lung and larynx enhances global methylation of DNA. Proc Annu Meet Am Assoc Cancer Res 41:497, 2000.
26.	Wenstrom KD, DuBard M, Owen J, Johanning G, Tamura T. The relationship of amniotic fluid homocysteine and the 677C®T MTHFR mutation to spontaneous pregnancy loss in women with a history of NTD. Am J Obstet Gynecol 182:S190, 2000.

Grant & Contract Support

Title:	Breast Cancer Screening by Assessing Human Endogenous Retrovirus-K (HERV-K) Antibodies and RNA
Funding Source:	Avon Foundation
Role:	Principal Investigator
Duration:	3/1/2010 - 2/28/2012

Title:	Evaluation of Radiation-Sensitivity in the Macaque Mammary Stem Cells
Funding Source:	The University of Texas MD Anderson Cancer Center, Cattlemen for Cancer Research Internal Funding Allocation
Role:	Principal Investigator
Duration:	11/1/2009 - 10/31/2012

Title:	The Impact of Gestational and Postnatal Exposure on Metabolic Programming and Breast Cancer Risk
Funding Source:	Department of Defense (DOD)
Role:	Co-Investigator
Principal Investigator:	Fuchs-Young
Duration:	9/15/2008 - 10/14/2011

Title:	Breast Cancer Screening by Assessing Human Endogenous Retrovirus-K (HERV-K) Antibodies and RNA
Funding Source:	Avon Foundation
Role:	Principal Investigator
Duration:	1/1/2008 - 12/31/2010

Title:	Obesity and Breast Cancer in a Mouse Model
Funding Source:	The University of Texas MD Anderson Cancer Center Cattlemen for Cancer Research Internal Funding Allocation
Role:	Principal Investigator
Duration:	10/1/2007 - 10/30/2010

Title:	Folic Acid and Antigen-Specific Cellular Immunity
Funding Source:	The University of Texas MD Anderson Cancer Center, Cattlemen for Cancer Research Internal Funding Allocation
Role:	Principal Investigator
Duration:	6/1/2006 - 5/31/2008

Title:	Acyl Co-A Binding Protein Expression in Cancer
Funding Source:	The University of Texas MD Anderson Cancer Center, Cattlemen for Cancer Research Internal Funding Allocation
Role:	Principal Investigator
Duration:	10/1/2004 - 10/31/2009

Title:	The Role(s) of Human Endogenous Retrovirus Expression in Ovarian Cancer
Funding Source:	The University of Texas MD Anderson Cancer Center
Role:	Investigator
Principal Investigator:	Wang-Johanning
Duration:	1/1/2002 - 12/13/2002

Last updated: 11/5/2012