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Howard B. Gutstein, M.D.

Present Title & Affiliation

Primary Appointment

Professor, Department of Anesthesiology and Perioperative Medicine, Division of Anesthesiology and Critical Care, The University of Texas MD Anderson Cancer Center, Houston, TX

Dual/Joint/Adjunct Appointment

Professor, Department of Biochemistry and Molecular Biology, The University of Texas, MD Anderson Cancer Center, Houston, TX

Research Interests

  • Developing novel analytical methods and technologies for the identification of proteomic biomarkers of addiction, opioid tolerance, and cancer pain from small groups of cells in the brain and spinal cord.  High risk studies that have already begun to make a significant impact. 
  •  Exploring novel signaling mechanisms and epigenetic changes relevant to cancer pain and addition - initial results appear to be very high impact.

Office Address

The University of Texas MD Anderson Cancer Center
1515 Holcombe Boulevard
Department of Anesthesiology & Perioperative Medicine - Research
Unit Number: 110
Houston, TX 77030
Room Number: Y6.5613
Phone: 713/792-5037
Fax: 713/794-4590

Education & Training

Degree-Granting Education

1982 Johns Hopkins University School of Medicine, Baltimore, MD, MD, Medicine
1977 Johns Hopkins University School of Medicine, Baltimore, MD, BA, Human Biology

Postgraduate Training

2012-2013 Graduate Certification in, Healthcare Management, Jones School of Business, Rice University, Houston, TX

Board Certifications

10/1993 Added Qualification in Pain Management, American Board of Anesthesiology
10/1988 American Board of Anesthesiology


Administrative Appointments/Responsibilities

Director, Pediatric Pain Section, Department of Anesthesiology and Pain Medicine, Division of Anesthesiology and Critical Care, The University of Michigan, Ann Arbor, MI, 1993-1998

Other Appointments/Responsibilities

Member, University of Texas - Houston, Kroc Family Fellowship Selection Committee, Houston, TX, 2008


Member, Reuters Insight, Community of Experts, New York, NY, 2008-present

Institutional Committee Activities

Member, Clinical Translational Research Center (CTRC) oversight committee, 1999-2000

Honors and Awards

2012 Heavner Lectureship, Texas Pain Society
2008-2009 Faculty Leadership Academy, U T M D Anderson Cancer Center
2008 Castle Connolly Top Doctors
1999 Featured participant, "Career Pathways in Behavioral Neuroscience" Symposium, National Institute on Drug Abuse
1996 "Featured participant, The Next Generation Symposium", National Institute on Drug Abuse
1994 Invited participant, NIH Workshop on Biobehavioral Pain Research, National Institute of Health
1993 Young Investigator Award, Foundation for Anesthesia Education and Research
1982 NIH Research Fellow, Johns Hopkins University
1981-1982 Vice-President, Class of 1982, Johns Hopkins Medical School
1979 NIH Research Fellow, Johns Hopkins University
1977 Accelerated B.A./M.D. Program, Johns Hopkins University
1975-1977 Chancellor's Scholarship, Washington University
1975-1976 Dean's List, Washington University
1975 Young Artists Program, Tanglewood Institute

Selected Publications

Peer-Reviewed Original Research Articles

1. Wang Y, Barker K, Shi S, Diaz M, Mo B, Gutstein HB. Blockade of PDGFR-β activation eliminates morphine analgesic tolerance. Nat Med 18(3):385-7, 3/2012. e-Pub 2/19/2012. PMCID: PMC3296828.
2. Babcock DT, Shi S, Jo J, Shaw M, Gutstein HB, Galko MJ. Hedgehog signaling regulates nociceptive sensitization. Curr Biol 21(18):1525-33, 9/27/2011. e-Pub 9/8/2011. PMCID: PMC3262399.
3. Morris JS, Baladandayuthapani V, Herrick RC, Sanna P, Gutstein H. Automated analysis of quantitative image data using isomorphic functional mixed models with application to proteomics data. Ann Appl Stat 5(2A):894-923, 1/2011. PMCID: PMC3298181.
4. Shurman J, Koob GF, Gutstein HB. Opioids, pain, the brain, and hyperkatifeia: a framework for the rational use of opioids for pain. Pain Med 11(7):1092-8, 7/2010. e-Pub 6/2010. PMCID: PMC2907890.
5. Morris JS, Clark BN, Wei W, Gutstein HB. Evaluating the performance of new approaches to spot quantification and differential expression in 2-dimensional gel electrophoresis studies. J Proteome Res 9(1):595-604, 1/2010. PMCID: PMC2802214.
6. Dowsey AW, Morris JS, Gutstein HB, Yang GZ. Informatics and statistics for analyzing 2-D gel electrophoresis images. Methods Mol Biol 604:239-55, 2010. PMID: 20013375.
7. Morris JS, Baggerly KA, Gutstein HB, Coombes KR. Statistical contributions to proteomic research. Methods Mol Biol 641:143-66, 2010. PMID: 20407946.
8. Gibson F, Anderson L, Babingg G, Baker M, Berth M, Binz PA, Borthwick A, Cash P, Day BW, Friedman DB, Garland D, Gutstein HG, Hoogland C, Jones NA, Jones AR, Khan A, Klose J, Lamond AL, Lemkin PR, Lilliey KS, Minden J, Morris NJ, Paton NW, Pisano MR, Prime JE, Rabilloud T, Stead DA, Taylor CF, Voshol H, Wipat A. MIAPE: Gel electrophoress Guidelines for reporting the use of gel electrophoresis in proteomics. Nature Biotechnology 26(8):863-4, 8/2008.
9. Gutstein HB, Morris JS, Annangudi SP, Sweedler JV. Miroproteomics: Analysis of protein diversity in small samples. Mass Spectrom Rev 27(4):316, Jul-Aug, 7/2008. e-Pub 2/2008. PMCID: PMC2743962.
10. Hamacher M, Eisenacher M, Tribl F, Stephan C, Marcus K, Hardt T, Wiltfang J, Martens L, Desiderio D, Gutstein H, Park YM, Meyer HE. The HUPO Brain Proteome project wish list--summary of the 9(th) HUPO BPP Workshop 9-10 January 2008, Barbados. Proteomics 8(11):2160-4, 6/2008. PMID: 18452233.
11. Clark BN, Gutstein HB. The myth of automated, high-throughput two-dimensional gel analysis. Proteomics 8(6):1197-203, 3/2008. e-Pub 2/2008. PMID: 18283661.
12. Morris JS, Clark BN, Gutstein HB. Pinnacle: a fast, automatic and accurate method for detecting and quantifying protein spots in 2-dimensional gel electrophoresis data. Bioinformatics 24(4):529-536, 2/2008. e-Pub 1/2008. PMCID: PMC2662725.
13. Moulédous L, Díaz MF, Gutstein HB. Extracellular signal-regulated kinase (ERK) inhibition does not prevent expression of tolerance to and dependence on morphine in the mouse. Pharmacol Biochem Behav 88(1)(1):39-46, 11/2007. e-Pub 7/18/2007. PMCID: PMC2151045.
14. Gutstein HB, Morris JS. Laser capture sampling and analytical issues in proteomics. Expert Rev Proteomics 4(5):627-37, 10/2007. PMCID: PMC2639751.
15. Hamacher M, Stephan C, Eisenacher M, van Hall A, Marcus K, Martens L, Park YM, Gutstein HB, Herberg F, Meyer HE. Proteomics for everyday use: Activities of the HUPO Brain Proteome Project during the 5(th) HUPO World Congress. Proteomics 7(7)(7):1012-5, 4/2007. PMID: 17340642.
16. Xu JJ, Walla BC, Diaz MF, Fuller GN, Gutstein HB. Intermittent lumbar puncture in rats: a novel method for the experimental study of opioid tolerance. Anesth Analg 103(3):714-20, 9/2006. PMID: 16931686.
17. Moulédous, L, Hunt, S, Harcourt, R, Harry, J, Williams, KL, Gutstein, HB. Proteomic changes in the membrane fraction of SH-SY5Y cells induced by fentanyl treatment. Journal of Cancer Pain and Symptom Palliation 1:35-43, 2005.
18. Moulédous L, Diaz MF, Gutstein HB. Modulation of extracellular signal-regulated kinase (ERK) activity by acute and chronic opioid treatment in neuronal and glial cell lines. J Neurochem 90(6):1371-7, 9/2004. PMID: 15341521.
19. Gutstein HB. Mechanisms underlying cancer-induced symptoms. Drugs Today (Barc) 39(10):815-22, 10/2003. PMID: 14668936.
20. Moulédous L, Hunt S, Harcourt R, Harry J, Williams KL, Gutstein HB. Navigated laser capture microdissection as an alternative to direct histological staining for proteomic analysis of brain samples. Proteomics 3(5):610-5, 5/2003. PMID: 12748941.
21. Cha EY, Moulédous L, Harris JR, Weech MA, Gutstein HB. Nitroglycerin inhibits the development of morphine tolerance and dependence in rats. Pharmacol Biochem Behav 74(3):551-7, 2/2003. PMID: 12543218.
22. Moulédous L, Hunt S, Harcourt R, Harry JL, Williams KL, Gutstein HB. Proteomic analysis of immunostained, laser-capture microdissected brain samples. Electrophoresis 24(1-2):296-302, 1/2003. PMID: 12652601.
23. Gutstein HB. In situ hybridization in neural tissues. Methods Mol Med 84:95-105, 2003. PMID: 12703320.
24. Mouledous L, Hunt S, Harcourt R, Harry JL, Williams KL, Gutstein HB. Lack of compatibility of histological staining methods with proteomic analysis of laser-capture microdissected brain samples . J Biomol Tech 13(4):258, 12/2002. PMCID: PMC2279876.
25. Bishop GB, Cullinan WE, Curran E, Gutstein HB. Abused drugs modulate RGS4 mRNA levels in rat brain: comparison between acute drug treatment and a drug challenge after chronic treatment. Neurobiol Dis 10:334-43, 8/2002. PMID: 12270694.
26. Gutstein HB. The biologic basis of fatigue. Cancer 92:1678-83, 9/2001. PMID: 11598886.
27. Phan D, Han E, Birrell G, Bonnal S, Duggan L, Esumi N, Gutstein H, Li R, Lopato S, Manogaran A, Pollak ES, Ray A, Reddi PP, Reichert AS, Struffi P, Tiscornia G, Ximenez-Fyvie LA, Zhang H, Lin SH. Purification and characterization of human cell--cell adhesion molecule 1 (C-CAM1) expressed in insect cells. Protein Expr Purif 21:343-51, 3/2001. PMID: 11237697.
28. Merkel SI, Gutstein HB, Malviya S. Use of transcutaneous electrical nerve stimulation in a young child with pain from open perineal lesions. J Pain Symptom Manage 18(5):376-81, 11/1999. PMID: 10584462.
29. Akil H, Owens C, Gutstein H, Taylor L, Curran E, Watson S. Endogenous opioids: overview and current issues. Drug Alcohol Depend 51920:127-40, 6/1998. PMID: 9716935.
30. Gutstein HB, Mansour A, Watson SJ, Akil H, Fields HL. Mu and kappa opioid receptors in periaqueductal gray and rostral ventromedial medulla. Neuroreport 9(8):1777-81, 6/1998. PMID: 9665599.
31. Gutstein, HB, Thome, JL, Fine, JL, Watson, SJ, Akil, H. The pattern of c-fos mRNA induction in rat brain by acute morphine. Can J Physiol Pharmacol 76(3):294-303, 3/1998. PMID: 9673793.
32. Snow BE, Antonio L, Suggs S, Gutstein HB, Siderovski DP. Molecular cloning and expression analysis of rat Rgs12 and Rgs14. Biochem Biophys Res Commun 233:770-777, 4/1997. PMID: 9168931.
33. Gutstein HB, Trujillo KA. MK-801 inhibits the development of morphine tolerance at spinal sites. Brain Res 626:332-334, 10/1993. PMID: 8281446.

Invited Articles

1. Gutstein HB. Pediatric Intubation. Anesthesia and Analgesia 89:807, 1999.
2. Wang Y, XU JJ, Cao F, Shi S, Mo B, Gustein, HB. Gabapentin blocks morphine tolerance by limiting PDGF-β activation. Science. Submitted.


1. Monroe EB, Losh JL, Hatcher NG, Gutstein HB, Sweedler JV. A multi-faceted approach to the mass spectrometric imaging of spinal cord sections. Proceedings of: American Society of Mass Spectrometry Meeting, 2007.
2. Xu JJ, Clark BN, Diaz M, Gutstein HB. Does spinal co-administration with sub-analgesic doses of DAMGO inhibit the development of morphine tolerance?. Proceedings of: International Narcotics Research Conference M3, 2005.
3. Barker K, Gutstein H. Does systemic co-administration of morphine with sub-analgesic doses of fentanyl inhibit the development of opioid tolerance?. Proceedings of: International Narcotics Research Conference M5, 2005.
4. Clark BN, Xu JJ, Diaz M, Gutstein HB. Repeated lumbar puncture in rats: A novel method for the experimental study of opioid tolerance. Proceedings of: International Narcotics Research Conference M4, 2005.
5. Gutstein, HB, Moulédous, L, Hunt, S, Harcourt, R, Harry, J, Williams, KL. Proteome Alterations Induced by Chronic Fentanyl Treatment in Human SH-SY5Y Neuroblastoma Cells. Anesthesiology 99:A802, 2003.
6. Moulédous, L, Hunt, S, Harcourt, R, Harry, J, Williams, KL, Gutstein, HB. Proteome alterations induced by fentanyl treatment in SH-SY5Y neuroblastoma cells. Society for Neuroscience Abstracts 28:206.4, 2002.
7. Bishop, GB, Curran, EJ, Watson, SJ, Akil, H, Gutstein, HB. Acute treatment with opioids and stimulants alter RGS 4 mRNA levels in rat brain. Anesthesiology 93:A127, 2000.
8. Bishop, GB, Curran, EJ, Watson, SJ, Akil, H, Gutstein, HB. Chronic administration of opioids and psychostimulants alter RGS 4 mRNA levels in rat brain. Anesthesiology 93:A779, 2000.
9. Gutstein, HB, Bishop, GB, Curran, EJ, Watson, SJ, Akil, H. Chronic morphine and psychostimulants alter RGS 4 levels in rat brain. Society for Neuroscience Abstracts 26:49.20, 2000.
10. Gutstein HB. Do opioids acutely activate ERK in neuronal cell lines? . Anesthesiology 93:A128, 2000.
11. Pearson, PP, Bishop, GB, Trzaskos, JM, Gutstein, HB. ERK inhibition reduces opioid tolerance in rats. Anesthesiology 93:A795, 2000.
12. Pearson, PP, Bishop, GB, Trzaskos, JM, Gutstein, HB. ERK inhibition reduces opioid tolerance in rats. Society for Neuroscience Abstracts 26:400.13, 2000.
13. Gutstein, HB, Zhang, H. Is opioid tolerance a unitary phenomenon? Insights from proteomics. Anesthesiology 93:A782, 2000.
14. Gutstein HB. Opioid withdrawal activates ERK in N2A neuroblastoma cells: A potential role for ERK signaling in opioid dependence and withdrawal. Anesthesiology 93:A783, 2000.
15. Gutstein, HB, Zhang, H. The use of proteomics to identify molecular markers of opioid tolerance. Anesthesiology 93:A125, 2000.
16. Gutstein, HB, Cullinan, WE, Watson, SJ, Akil, H. Does the intensity of noxious stimulation influence c-fos activation patterns in rat brain?. Anesthesia and Analgesia 86:S273, 1998.

Book Chapters

1. Gutstein HB, Akil H. Opioid analgesics. In: Goodman and Gilman's The Pharmacological Basis of Therapeutics, Eleventh. Ed(s) L Brunton. McGraw-Hill, 547-590, 2006.
2. Hill CS, Jr, Cleeland CS, Gutstein HB. Effective Pain Treatment in Cancer Patients. In: The American Cancer Society's Clinical Oncology. Ed(s) RE Lenhard, TR Osteen, T Ganslre. American Cancer Society, 765-809, 2001.
3. Gutstein HB, Akil H. Opioid Analgesics. In: Goodman and Gilman's The Pharmacological Basis of Therapeutics, Tenth. Ed(s) J Hardman, L Limbird. McGraw-Hill, 569-619, 2001.
4. Gutstein, HB, Zaki, AF. Opioids. In: Anesthesiology: Pearls of Wisdom, First. Ed(s) KM Roberston, DA Lubarsky. Boston Medical Publishing, 211 - 216, 2001.
5. Gutstein HB. Use of the Bullard laryngoscope and light wand for difficult pediatric intubation. In: Anesthesiology Clinics of North America. 16(4). Ed(s) J Riazi, 795-812, 1998.

Grant & Contract Support

Title: Anti-Cancer Drugs to Treat Cancer Pain
Funding Source: Cancer Prevention & Research Institute of Texas (CPRIT)
Role: Principal Investigator
Duration: 2012 - 2015
Title: Growth Factor Receptor Inhibition to Block Opioid Tolerance and Treat Pain and Addiction
Funding Source: MD Anderson Cancer Center Techonology Review Committee
Role: Principal Investigator
Duration: 2010
Title: Transition From Drug Use to Addiction-Unearthing the Switches
Funding Source: NIH/NIDA
Role: Principal Investigator
Duration: 9/30/2002 - 5/31/2007
Title: Laboratory Studies of Pain Control Methods
Funding Source: National Cancer Institute
Role: Co-Investigator
Principal Investigator: C Cleeland
Duration: 9/1/1996 - 8/31/2001
Title: Behavioral and Neural Correlates of Pain and Analgesia
Funding Source: National Institute on Drug Abuse
Role: Principal Investigator
Duration: 9/30/1994 - 8/31/2000
Title: Neural Mechanisms of Pain Responsiveness
Funding Source: Foundation for Anesthesia Education and Research
Role: Principal Investigator
Duration: 7/1/1993 - 6/1/1995

Last updated: 1/28/2015