Skip to Content

'
Jianjun Shen, Ph.D.

Present Title & Affiliation

Primary Appointment

Professor, Department of Epigenetics and Molecular Carcinogenesis, Division of Basic Science Research, The University of Texas MD Anderson Cancer Center, Smithville, TX

Dual/Joint/Adjunct Appointment

Guest Professor, Medical School of Southeast University, Nanjing, China

Research Interests

Pancreatic cancer, early detection
Molecular mechanisms of obesity-associated pancreatic cancer risk
Proteomics
Next-generation sequencing

As Director of the Molecular Biology Facility and CPRIT-funded Next-Generation Sequencing (NGS) Facility at MD Anderson Cancer Center–Science Park, I consult, advise and collaborate with research investigators from The University of Texas MD Anderson, The University of Texas at Austin (UT-Austin), Texas State University-San Marcos, and Texas A&M Health Science Center Institute of Biosciences and Technology.  My research efforts thus focus on the development and utilization of molecular biology, proteomic and genomic (including NGS) techniques to study cancer and other environmental disease(s). In this regard, I am working with many collaborators on a number of research projects.

Among these, I am particularly interested in identifying potential biomarkers for early detection of pancreatic cancer. Pancreatic cancer is a rapidly progressing disease that has the lowest 5-year survival rate of all human malignancies, and at present, no effective treatment. Long-term type-II diabetes is recognized as a risk factor. Screening the higher-risk group of patients with new-onset diabetes for the early detection of pancreatic cancer has great clinical implications. In this regard, working with Dr. Donghui Li at MD Anderson and Dr. Maria Person at UT-Austin, I am profiling expression of glycopeptides and microRNA in individuals with recent onset of diabetes (< 2 years), with and without pancreatic cancer. The molecules identified as differentially expressed between the patient groups may hold promise as biomarkers for early detection of pancreatic cancer.

Obesity is another risk factor and a predictor for reduced survival in pancreatic cancer. However, the mechanisms underlying the association of obesity with pancreatic cancer are not yet known. Working with Drs. Susan Fischer and Joyce Rundhaug at MD Anderson–Science Park, I have employed next-generation sequencing technologies, including RNA-seq, microRNA-seq and DNA methylation profiling, to characterize a Panc02 pancreatic tumor xenograft mouse model. These profiling experiments will allow us to identify genetic, epigenetic and signaling changes that correlate obesity and energy balance to pancreatic cancer risk, potentially leading to the implementation of molecular-based chemoprevention strategies.

Office Address

Email: jianshen@mdanderson.org

Education & Training

Degree-Granting Education

1992 Rutgers, The State University of New Jersey, Newark, NJ, PHD, Physical Biochemistry
1990 Rutgers, The State University of New Jersey, Newark, NJ, MS, Chemistry
1983 Southeast University, Nanjing, China, BS, Chemistry

Experience/Service

Academic Appointments

Associate Member, The University of Texas Graduate School of Biomedical Sciences at Houston (GSBS), Houston, TX, 8/2011-present
Associate Professor, Department of Molecular Carcinogenesis, Division of Basic Science Research, The University of Texas MD Anderson Cancer Center, Smithville, TX, 9/2006-8/2013
Facility Core Director, Department of Molecular Carcinogenesis, Division of Basic Science Research, The University of Texas MD Anderson Cancer Center, Smithville, TX, 8/1998-present

Selected Publications

Peer-Reviewed Original Research Articles

1. Weeks JR, Hardin SE, Shen J, Lee JM, Greenleaf AL. Locus-specific variation in phosphorylation state of RNA polymerase II in vivo: correlations with gene activity and transcript processing. Genes Dev 7(12A):2329-44, 12/1993. PMID: 8253380.
2. Shen J, Bao Y, Liu HM, Lee P, Leonard JV, Chen YT. Mutations in exon 3 of the glycogen debranching enzyme gene are associated with glycogen storage disease type III that is differentially expressed in liver and muscle. J Clin Invest 98(2):352-7, 7/1996. PMCID: PMC507437.
3. McConkie-Rosell A, Wilson C, Piccoli DA, Boyle J, DeClue T, Kishnani P, Shen JJ, Boney A, Brown B, Chen YT. Clinical and laboratory findings in four patients with the non-progressive hepatic form of type IV glycogen storage disease. J Inherit Metab Dis 19(1):51-8, 1996. PMID: 8830177.
4. Shen J, Liu HM, Bao Y, Chen YT. Polymorphic markers of the glycogen debranching enzyme gene allowing linkage analysis in families with glycogen storage disease type III. J Med Genet 34(1):34-8, 1/1997. PMCID: PMC1050844.
5. Parvari R, Moses S, Shen J, Hershkovitz E, Lerner A, Chen YT. A single-base deletion in the 3'-coding region of glycogen-debranching enzyme is prevalent in glycogen storage disease type IIIA in a population of North African Jewish patients. Eur J Hum Genet 5(5):266-70, 9/1997. PMID: 9412782.
6. Shen J, Bao Y, Chen YT. A nonsense mutation due to a single base insertion in the 3'-coding region of glycogen debranching enzyme gene associated with a severe phenotype in a patient with glycogen storage disease type IIIa. Hum Mutat 9(1):37-40, 1997. PMID: 8990006.
7. Shen J, Liu HM, McConkie-Rosell A, Chen YT. Prenatal diagnosis and carrier detection for glycogen storage disease type III using polymorphic DNA markers. Prenat Diagn 18(1):61-4, 1/1998. PMID: 9483641.
8. Parvari R, Shen J, Hershkovitz E, Chen YT, Moses SW. Two new mutations in the 3' coding region of the glycogen debranching enzyme in a glycogen storage disease type IIIa Ashkenazi Jewish patient. J Inherit Metab Dis 21(2):141-8, 4/1998. PMID: 9584265.
9. Hadjigeorgiou GM, Comi GP, Bordoni A, Shen J, Chen YT, Salani S, Toscano A, Fortunato F, Lucchiari S, Bresolin N, Rodolico C, Piscaglia MG, Franceschina L, Papadimitriou A, Scarlato G. Novel donor splice site mutations of AGL gene in glycogen storage disease type IIIa. J Inherit Metab Dis 22(6):762-3, 8/1999. PMID: 10472540.
10. Shen J, Liu HM, McConkie-Rosell A, and Chen YT. Prenatal diagnosis for glycogen storage disease type IV, branching enzyme deficiency, using PCR-based DNA analysis. Prenat Diagn 19(9):837-9, 9/1999. PMID: 10521841.
11. Shaiu WL, Kishnani PS, Shen J, Liu HM, Chen YT. Genotype-phenotype correlation in two frequent mutations and mutation update in type III glycogen storage disease. Mol Genet Metab 69(1):16-23, 1/2000. PMID: 10655153.
12. Shen JJ, Matern D, Millington DS, Hillman S, Feezor MD, Bennett MJ, Qumsiyeh M, Kahler SG, Chen YT, Van Hove JL. Acylcarnitines in fibroblasts of patients with long-chain 3-hydroxyacyl-CoA dehydrogenase deficiency and other fatty acid oxidation disorders. J Inherit Metab Dis 23(1):27-44, 2/2000. PMID: 10682306.
13. Van Hove JL, Kahler SG, Feezor MD, Ramakrishna JP, Hart P, Treem WR, Shen JJ, Matern D, Millington DS. Acylcarnitines in plasma and blood spots of patients with long-chain 3-hydroxyacyl-coenzyme A dehydrogenase defiency. J Inherit Metab Dis 23(6):571-82, 9/2000. PMID: 11032332.
14. Yan M, Qiang W, Liu N, Shen JJ, Lynn WS, Wong PK. The ataxia-telangiectasia gene product may modulate DNA turnover and control cell fate by regulating cellular redox in lymphocytes. Faseb J 15(7):1132-8, 5/2001. PMID: 11344081.
15. Li D, Zhang W, Zhu J, Chang P, Sahin A, Singletary E, Bondy M, Hazra T, Mitra S, Lau S, Shen J, DiGiovanni J. Oxidative DNA damage and 8-hydroxy-2-deoxyguanosine DNA glycosylase/apurinic lyase in human breast cancer. Mol Carcinog 31(4):214-23, 8/2001. PMID: 11536371.
16. Kiguchi K, Carbajal S, Chan K, Beltran L, Ruffino L, Shen J, Matsumoto T, Yoshimi N, DiGiovanni J. Constitutive expression of ErbB-2 in gallbladder epithelium results in development of adenocarcinoma. Cancer Res 61(19):6971-6, 10/2001. PMID: 11585718.
17. Xu H, Shen J, Walker CL, Kleymenova E. Tissue-specific expression and splicing of the rat polycystic kidney disease 1 gene. DNA Seq 12(5-6):361-6, 12/2001. PMID: 11913782.
18. Li D, Firozi PF, Zhang W, Shen J, DiGiovanni J, Lau S, Evans D, Friess H, Hassan M, Abbruzzese JL. DNA adducts, genetic polymorphisms and K-ras mutation in human pancreatic cancer. Mutation Research 513(1-2):37-48, 1/2002. PMID: 11719088.
19. Tang S, Bhatia B, Maldonado CJ, Yang P, Newman RA, Liu J, Chandra D, Traag J, Klein RD, Fischer SM, Chopra D, Shen J, Zhau HE, Chung LW, Tang DG. Evidence that arachidonate 15-lipoxygenase 2 is a negative cell cycle regulator in normal prostate epithelial cells. Journal of Biological Chemistry 277(18):16189-201, 5/2002. PMID: 11839751.
20. Vanzulli1 S, Efeyan A, Benavides F, Helguero L, Peters G, Shen J, Conti CJ, Lanari C, Molinolo A. p21, p27 and p53 in estrogen and antiprogestin-induced tumor regression of experimental mouse mammary ductal carcinoma. Carcinogenesis 23(5):749-57, 5/2002. PMID: 12016147.
21. Yan M, Kuang X, Qiang W, Shen J, Claypool, K, Lynn WS, Wong PK. Prevention of thymic lymphoma development in atm-/- mice by dexamethasone. Cancer Res 62(18):5153-7, 9/2002. PMID: 12234978.
22. Kochan KJ, Wright DA, Schroeder LJ, Shen J, Morizot DC. Genetic linkage maps of the West African clawed frog Xenopus tropicalis. Dev Dyn 226(1):99-102, 1/2003. PMID: 12508229.
23. Liu JW, Shen JJ, Tanzillo-Swarts A, Bhatia B, Maldonado CM, Person MD, Lau SS, Tang DG. Annexin II expression is reduced or lost in prostate cancer cells and its re-expression inhibits prostate cancer cell migration. Oncogene 22(10):1475-85, 3/2003. PMID: 12629510.
24. Lopes EC, Garcia M, Benavides F, Shen J, Conti CJ, Alvarez E, Hajos SE. Multidrug resistance modulators PSC 833 and CsA show differential capacity to induce apoptosis in lymphoid leukemia cell lines independently of their MDR phenotype. Leukemia Research 27(5):413-23, 5/2003. PMID: 12620293.
25. Yadav N, Lee J, Kim J, Shen J, Hu MC, Aldaz CM, Bedford MT. Specific protein methylation defects and gene expression perturbations in coactivator-associated arginine methyltransferase 1-deficient mice. Proc Natl Acad Sci U S A 100(11):6464-8, 5/2003. PMCID: PMC164469.
26. Soliman AS, Vulimiri SV, Kleiner HE, Shen J, Eissa S, Morad M, Taha H, Lukmanji F, Li D, Johnston DA, Lo HH, Lau S, DiGiovanni J, Bondy ML. High levels of oxidative DNA damage in lymphocyte DNA of premenopausal breast cancer patients from Egypt. Int J Environ Health Res 14(2):121-34, 4/2004. PMID: 15203457.
27. Jia Z, Person MD, Dong J, Shen J, Hensley SC, Stevens JL, Monks TJ, Lau SS. Grp78 is essential for 11-deoxy-16,16-dimethyl PGE2-mediated cytoprotection in renal epithelial cells. Am J Physiol Renal Physiol 287(6):F1113-22, 12/2004. PMID: 15226156.
28. Shen J, Person MD, Zhu J, Abbruzzese JL, Li D. Protein expression profiles in pancreatic adenocarcinoma compared with normal pancreatic tissue and tissue affected by pancreatitis as detected by two-dimensional gel electrophoresis and mass spectrometry. Cancer Res 64(24):9018-26, 12/2004. PMID: 15604267.
29. Cai SL, Tee AR, Short JD, Bergeron JM, Kim J, Shen J, Guo R, Johnson CL, Kiguchi K, Walker CL. Activity of TSC2 is inhibited by AKT-mediated phosphorylation and membrane partitioning. J Cell Biol 173(2):279-89, 4/2006. PMCID: PMC2063818.
30. Person MD, Shen J, Traner A, Hensley SC, Lo HH, Abbruzzese JL, Li D. Protein fragment domains identified using 2D gel electrophoresis/MALDI-TOF. J Biomol Tech 17(2):145-56, 4/2006. PMCID: PMC2291776.
31. Liu B, Park E, Zhu F, Bustos T, Liu J, Shen J, Fischer SM, Hu Y. A critical role for I kappaB kinase alpha in the development of human and mouse squamous cell carcinomas. Proc Natl Acad Sci U S A 103(46):17202-7, 11/2006. PMCID: PMC1859910.
32. Shen J, Pavone A, Mikulec C, Hensley SC, Traner A, Chang TK, Person MD Fischer SM. Protein expression profiles in the epidermis of cyclooxygenase-2 transgenic mice by 2-dimensional gel electrophoresis and mass spectrometry. J Proteome Res 6(1):273-86, 1/2007. PMID: 17203971.
33. Shen J, Riggs PK, Hensley SC, Schroeder LJ, Traner AR, Kochan KJ, Person MD, DiGiovanni J. Differential expression of multiple anti-apoptotic proteins in epidermis of IGF-1 transgenic mice as revealed by 2-dimensional gel electrophoresis/mass spectrometry analysis. Mol Carcinog 46(5):331-40, 5/2007. PMID: 17330866.
34. Yan M, Kuang X, Scofield VL, Shen J, Lynn WS, Wong PK. The glucocorticoid receptor is increased in Atm-/- thymocytes and in Atm-/- thymic lymphoma cells, and its nuclear translocation counteracts c-myc expression. Steroids 72(5):415-21, 5/2007. PMID: 17418878.
35. Zhu F, Xia X, Liu B, Shen J, Hu Y, Person M, Hu Y. IKKalpha shields 14-3-3sigma, a G(2)/M cell cycle checkpoint gene, from hypermethylation, preventing its silencing. Mol Cell 27(2):214-27, 7/2007. PMID: 17643371.
36. Morrison AJ, Kim JA, Person MD, Highland J, Xiao J, Wehr TS, Hensley S, Bao Y, Shen J, Collins SR, Weissman JS, Delrow J, Krogan NJ, Haber JE, Shen X. Mec1/Tel1 phosphorylation of the INO80 chromatin remodeling complex influences DNA damage checkpoint responses. Cell 130(3):499-511, 8/2007. PMID: 17693258.
37. Fan T, Li R, Todd NW, Qiu Q, Fang HB, Wang H, Shen J, Zhao RY, Caraway NP, Katz RL, Stass SA, Jiang F. Up-regulation of 14-3-3Ƀ in lung cancer and its implication as prognostic and therapeutic target. Cancer Res 67(16):7901-6, 8/2007. PMID: 17699796.
38. Park E, Zhu F, Liu B, Xia X, Shen J, Bustos T, Fischer SM, Hu Y. Reduction in IkappaB kinase alpha expression promotes the development of skin papillomas and carcinomas. Cancer Res 67(19):9158-68, 10/2007. PMID: 17909021.
39. Yan M, Shen J, Person MD, Kuang X, Lynn WS, Atlas D, Wong PK. Endoplasmic reticulum stress and unfolded protein response in Atm-deficient thymocytes and thymic lymphoma cells are attributable to oxidative stress. Neoplasia 10(2):160-7, 2/2008. PMCID: PMC2244691.
40. Chan KS, Sano S, Kataoka K, Abel E, Carbajal S, Beltran L, Clifford J, Peavey M, Shen J, DiGiovanni J. Forced expression of a constitutively active form of Stat3 in mouse epidermis enhances malignant progression of skin tumors induced by two-stage carcinogenesis. Oncogene 27(8):1087-94, 2/2008. PMID: 17700521.
41. Bhatia B, Multani AS, Patrawala L, Chen X, Calhoun-Davis T, Zhou J, Schroeder L, Schneider-Broussard R, Shen J, Pathak S, Chang S, Tang DG. Evidence that senescent human prostate epithelial cells enhance tumorigenicity: Cell fusion as a potential mechanism and inhibition by p16INK4a and hTERT. Int J Cancer 122(7):1483-95, 4/2008. PMID: 18059027.
42. Short JD, Houston KD, Dere R, Cai SL, Kim J, Johnson CL, Broaddus RR, Shen J, Miyamoto S, Tamanoi F, Kwiatkowski D, Mills GB, Walker CL. AMP-activated protein kinase signaling results in cytoplasmic sequestration of p27. Cancer Res 68(16):6496-506, 8/2008. PMCID: PMC3011867.
43. Kuang X, Shen J, Wong PK, Yan M. Deregulation of mTOR signaling is involved in thymic lymphoma development in Atm-/- mice. Biochem Biophys Res Commun 383(3):368-72, 6/2009. PMID: 19364503.
44. Alexander A, Cai SL, Kim J, Nanez A, Sahin M, MacLean KH, Inoki K, Guan KL, Shen J, Person MD, Kusewitt D, Mills GB, Kastan MB, Walker CL. ATM Signals to TSC2 in the cytoplasm to regulate mTORC1 in response to ROS. Proc Natl Acad Sci U S A 107(9):4153-8, 3/2010. e-Pub 2/2010. PMCID: PMC2840158.
45. Short JD, Dere R, Houston KD, Cai SL, Kim J, Bergeron JM, Shen J, Liang J, Bedford MT, Mills GB, Walker CL. AMPK-Mediated Phosphorylation of Murine p27 at T197 Promotes Binding of 14-3-3 Proteins and Increases p27 Stability. Mol Carcinog 49(5):429-39, 5/2010. e-Pub 2/2010. PMID: 20146253.
46. Benavides F, Perez C, Blando J, Contreras O, Shen J, Coussens LM, Fischer SM, Kusewitt DF, Digiovanni J, Conti CJ. Protective role of cathepsin L in mouse skin carcinogenesis. Mol Carcinog, 5/2011. PMCID: PMC3155742.
47. Jeter CR, Liu B, Liu X, Chen X, Liu C, Calhoun-Davis T, Repass J, Zaehres H, Shen JJ, Tang DG. NANOG promotes cancer stem cell characteristics and prostate cancer resistance to androgen deprivation. Oncogene 30(36):3833-45, 9/2011. e-Pub 4/2011. PMCID: PMC3140601.
48. Blando J, Moore T, Hursting S, Jiang G, Saha A, Beltran L, Shen J, Repass J, Strom S, Digiovanni J. Dietary energy balance modulates prostate cancer progression in hi-myc mice. Cancer Prev Res (Phila) 4(12):2002-14, 12/2011. e-Pub 9/2011. PMID: 21952584.
49. Yan L, Della Coletta L, Powell KL, Shen J, Thames H, Aldaz CM, MacLeod MC. Activation of the canonical Wnt/β-catenin pathway in ATF3-induced mammary tumors. PLoS One 6(1):e16515, 2011. e-Pub 1/2011. PMCID: PMC3031586.
50. Wang-Johanning F, Rycaj K, Plummer JB, Li M, Yin B, Frerich K, Garza JG, Shen J, Lin K, Yan P, Glynn SA, Dorsey TH, Hunt KK, Ambs S, Johanning GL. Immunotherapeutic potential of anti-human endogenous retrovirus-K envelope protein antibodies in targeting breast tumors. J Natl Cancer Inst 104(3):189-210, 2/2012. e-Pub 1/2012. PMCID: PMC3274512.
51. Shen J, Abel EL, Riggs PK, Repass J, Hensley SC, Schroeder LJ, Temple A, Chau A, McClellan SA, Rho O, Kiguchi K, Ward MD, Semmes OJ, Person MD, Angel JM, Digiovanni J. Proteomic and pathway analyses reveal a network of inflammatory genes associated with differences in skin tumor promotion susceptibility in DBA/2 and C57BL/6 mice. Carcinogenesis 33(11):2208-19, 11/2012. e-Pub 7/2012. PMCID: PMC3483013.
52. Xia X, Liu S, Xiao Z, Zhu F, Song NY, Zhou M, Liu B, Shen J, Nagashima K, Veenstra TD, Burkett S, Datla M, Willette-Brown J, Shen H, Hu Y. An IKKα-Nucleophosmin Axis Utilizes Inflammatory Signaling to Promote Genome Integrity. Cell Rep 5(5):1243-1255, 12/2013. e-Pub 11/2013. PMID: 24290756.
53. Liu B, Badeaux MD, Choy G, Chandra D, Shen I, Jeter CR, Rycaj K, Lee CF, Person MD, Liu C, Chen Y, Shen J, Jung SY, Qin J, Tang DG. Nanog1 in NTERA-2 and recombinant NanogP8 from somatic cancer cells adopt multiple protein conformations and migrate at multiple M.W species. PLoS One 9(3):e90615, 2014. e-Pub 3/2014. PMCID: PMC3944193.
54. Simper MS, Rundhaug JE, Mikulec C, Bowen R, Shen J, Lu Y, Lin K, Surh I, Fischer SM. The tumor promoting activity of the EP4 receptor for prostaglandin E2 in murine skin. Mol Oncol. e-Pub 7/2014. PMID: 25034079.

Last updated: 10/23/2014