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Richard G. Brennan, Ph.D.

Present Title & Affiliation

Primary Appointment

Professor and Robert A. Welch Distinguished University Chair in Chemistry, Department of Biochemistry and Molecular Biology, The University of Texas M. D. Anderson Cancer Center, Houston, TX

Dual/Joint/Adjunct Appointment

Adjunct Professor, Department of Biochemistry and Cell Biology, Rice University, Houston, TX

Research Interests

  Research Interests

  • Multidrug resistance
  • Gene regulation
  • Protein-ligand interaction
  • X-ray crystallography

Research Synopsis
The overarching goal of the Brennan laboratory is to understand gene regulation at its most fundamental level. Such understanding requires the complete delineation of the structural and biochemical mechanisms of ligand binding or reversible chemical modifications of a number of transcriptional regulators. Thus, these regulatory proteins act as direct environmental sensors and effect changes in the genetic program of the cell in order to respond to the presence of specific cytoplasmic signals or stresses. To date, we have solved the crystal structures of a number of transcription regulators and biologically germane protein-ligand complexes including PurR, which senses the levels of purines in the cell, bound to small molecule corepressors and DNA, a CREB bZIP-DNA complex and CcpA bound to the phosphoprotein HPr(Ser46P) and a catabolite responsive element. The latter multiprotein-DNA structure has elucidated the mechanism of carbon catabolite regulation (CCR) in bacilli and opened avenues to exploit CCR as a novel drug target. One key stress response, which is particularly relevant to human health, is the defence that cells muster upon their exposure to multiple drugs. The emergence of multidrug resistant (mdr) bacteria, fungi and tumour cells, has resulted in the failure of a number of chemotherapeutics to treat infections and certain cancers. In order to understand the origins of multidrug resistance, we are carrying out a number of biochemical and crystallographic studies on the multidrug binding transcription regulators, QacR, BmrR, MtrR and NfxB that will elucidate their DNA and multidrug binding mechanisms, and hence their modes of mdr gene regulation. As a logical extension, we have begun similar studies on several multidrug efflux transporters with the long-term goal of developing structure-based antibacterial chemotherapeutics. Work on HIF-1, Spx, OhrR and PerR will reveal the transcriptional responses of mammals to hypoxia, which is key for tumour growth, and bacteria to oxidative stress, which allow them to overcome the killing effects of peroxides and superoxide anions. Finally, we are interested in unveiling the mechanisms of post transcriptional gene regulation by small noncoding RNAs. To do so, we are carrying out structure-function studies on Hfq, the ubiquitous, pleiotropic regulator of translation and mRNA stability, and its physiologically relevant RNA complexes.

Office Address

The University of Texas M. D. Anderson Cancer Center
1515 Holcombe Boulevard
Unit Number: 1000
Houston, TX 77030
Room Number: S7.8336C
Phone: (713) 834-6390
Fax: (713) 834-6397
Email: rgbrenna@mdanderson.org

Education & Training

Degree-Granting Education

1984 University of Wisconsin, Madison, WI, PHD, Biochemistry
1977 Boston University, Boston, MA, BA, Biology

Experience/Service

Administrative Appointments/Responsibilities

Director, Center for Biomolecular Structure and Function (CBSF), The University of Texas M. D. Anderson Cancer Center, Houston, TX, 2008

Endowed Positions

Robert A. Welch Distinguished Chair in Chemistry, The University of Texas M. D. Anderson Cancer Center, Houston, TX, 2007

Selected Publications

Peer-Reviewed Original Research Articles
1. Newberry KJ, Huffman JL, Miller MC, Vazquez-Laslop N, Neyfakh AA, Brennan RG. Structures of BmrR-drug complexes reveal a rigid multidrug binding pocket and transcription activation through tyrosine expulsion. J Biol Chem, 7/25/2008. PMID: 18658145.
2. Shelburne SA, Keith D, Horstmann N, Sumby P, Davenport MT, Graviss EA, Brennan RG, Musser JM. A direct link between carbohydrate utilization and virulence in the major human pathogen group A Streptococcus. Proc Natl Acad Sci U S A 105(5):1698-703, 2/5/2008. PMID: 18230719.
3. Amen M, Espinoza HM, Cox C, Liang X, Wang J, Link TM, Brennan RG, Martin JF, Amendt BA. Chromatin-associated HMG-17 is a major regulator of homeodomain transcription factor activity modulated by Wnt/beta-catenin signaling. Nucleic Acids Res 36(2):462-76, 2/2008. PMID: 18045789.
4. Brooks BE, Piro KM, Brennan RG. Multidrug-binding transcription factor QacR binds the bivalent aromatic diamidines DB75 and DB359 in multiple positions. J Am Chem Soc 129(26):8389-95, 7/4/2007. PMID: 17567017.
5. Schumacher MA, Seidel G, Hillen W, Brennan RG. Structural mechanism for the fine-tuning of CcpA function by the small molecule effectors glucose 6-phosphate and fructose 1,6-bisphosphate. J Mol Biol 368(4):1042-50, 5/11/2007. PMID: 17376479.
6. Schumacher MA, Seidel GH, Brennan RG. Structural mechanism for the finetuning of CcpA function by the small molecule effectors glucose-6-phosphate and fructose-1,6-bisphosphate. J. Mol. Biol. 368:1042-1050, 2007.
7. Newberry KJ, Fuangthong M, Panmanee W, Mongkolsuk S, Brennan RG. Structural Mechanism of Organic Hydroperoxide Induction of the Transcription Regulator OhrR. Mol Cell 128(4):652-664, 2007. PMID: 18042459.
8. Brooks BE, Piro KM, Brennan RG. The Multidrug-Binding Transcription Factor, QacR, Binds the Bivalent Aromatic Diamidines DB75 and DB359 in Multiple Positions. J. Amer. Chem. Soc. 129:8389-8395, 2007.
9. Newberry, K, Huffman, JL, Miller, MC, Vazquez-Laslop, Neyfakh, AA, Brennan, RG. BmrR requires a buried glutamate for transcription regulation but not high affinity drug binding. Under revision, J. Biol. Chem, 2006.
10. Murray, DS, Schumacher, MA, Wray, Jr, LV, Fisher, SH, Brennan, RG. Crystal Structures of the GSI-a Glutamine Synthetase from Bacillus subtilis. J. Mol. Biol Accepted pending minor revisions, 2006.
11. Gang, J, Huffman, JL, Lutsenko, S, Lundblad, JR, Brennan, RG. DNA and metal specificity of the transcription regulator and MerR family member PMTR from Proteus mirabilis. J. Biol. Chem., under revision, 2006.
12. Schumacher MA, Seidel G, Hillen W, Brennan RG. Phosphoprotein Crh-Ser46-P displays altered binding to CcpA to effect carbon catabolite regulation. J Biol Chem 281(10):6793-800, 2006. PMID: 16316990.
13. Kliegman JI, Griner SL, Helmann JD, Brennan RG, Glasfeld A. Structural basis for the metal-selective activation of the manganese transport regulator of Bacillus subtilis. Biochemistry 45(11):3493-505, 2006. PMID: 16533030.
14. Mattison K, Wilbur JS, So M, Brennan RG. Structure of FitAB from Neisseria gonorrhoeae bound to DNA reveals a tetramer of toxin-antitoxin heterodimers containing pin domains and ribbon-helix-helix motifs. J Biol Chem 281(49):37942-51, 2006. PMID: 16982615.
15. DeWitt MA, Kliegman JI, Helmann JD, Brennan RG, Farrens DL, Glasfeld A. The Conformations of the Manganese Transport Regulator of Bacillus subtilis in its Metal-free State. J Mol Biol 365(5):1257-65, 2006. PMID: 17118401.
16. Hoffmann KM, Williams D, Shafer WM, Brennan RG. Characterization of the multiple transferable resistance repressor, MtrR, from Neisseria gonorrhoeae. J Bacteriol 187(14):5008-12, 2005. PMID: 15995218.
17. Newberry KJ, Nakano S, Zuber P, Brennan RG. Crystal structure of the Bacillus subtilis anti-alpha, global transcriptional regulator, Spx, in complex with the alpha C-terminal domain of RNA polymerase. Proc Natl Acad Sci U S A 102(44):15839-44, 2005. PMID: 16249335.
18. Wilbur JS, Chivers PT, Mattison K, Potter L, Brennan RG, So M. Neisseria gonorrhoeae FitA interacts with FitB to bind DNA through its ribbon-helix-helix motif. Biochemistry 44(37):12515-24, 2005. PMID: 16156663.
19. Hong M, Fuangthong M, Helmann JD, Brennan RG. Structure of an OhrR-ohrA operator complex reveals the DNA binding mechanism of the MarR family. Mol Cell 20(1):131-41, 2005. PMID: 16209951.
20. Murray DS, Schumacher MA, Brennan RG. Crystal structures of QacR-diamidine complexes reveal additional multidrug-binding modes and a novel mechanism of drug charge neutralization. J Biol Chem 279(14):14365-71, 2004. PMID: 14726520.
21. Schumacher MA, Allen GS, Diel M, Seidel G, Hillen W, Brennan RG. Structural basis for allosteric control of the transcription regulator CcpA by the phosphoprotein HPr-Ser46-P. Cell 118(6):731-41, 2004. PMID: 15369672.
22. Schumacher MA, Miller MC, Brennan RG. Structural mechanism of the simultaneous binding of two drugs to a multidrug-binding protein. EMBO J 23(15):2923-30, 2004. PMID: 15257299.
23. Newberry KJ, Brennan RG. The structural mechanism for transcription activation by MerR family member multidrug transporter activation, N terminus. J Biol Chem 279(19):20356-62, 2004. PMID: 14985361.
24. Allen GS, Steinhauer K, Hillen W, Stülke J, Brennan RG. Crystal structure of HPr kinase/phosphatase from Mycoplasma pneumoniae. J Mol Biol 326(4):1203-17, 2003. PMID: 12589763.
25. Glasfeld A, Guedon E, Helmann JD, Brennan RG. Structure of the manganese-bound manganese transport regulator of Bacillus subtilis. Nat Struct Biol 10(8):652-7, 2003. PMID: 12847518.
26. Steinhauer K, Allen GS, Hillen W, Stülke J, Brennan RG. Crystallization, preliminary X-ray analysis and biophysical characterization of HPr kinase/phosphatase of Mycoplasma pneumoniae. Acta Crystallogr D Biol Crystallogr 58(Pt 3):515-8, 2002. PMID: 11856840.
27. Møller T, Franch T, Højrup P, Keene DR, Bächinger HP, Brennan RG, Valentin-Hansen P. Hfq: a bacterial Sm-like protein that mediates RNA-RNA interaction. Mol Cell 9(1):23-30, 2002. PMID: 11804583.
28. Bashor C, Denu JM, Brennan RG, Ullman B. Kinetic mechanism of adenine phosphoribosyltransferase from Leishmania donovani. Biochemistry 41(12):4020-31, 2002. PMID: 11900545.
29. Huffman JL, Lu F, Zalkin H, Brennan RG. Role of residue 147 in the gene regulatory function of the Escherichia coli purine repressor. Biochemistry 41(2):511-20, 2002. PMID: 11781089.
30. Schumacher MA, Miller MC, Grkovic S, Brown MH, Skurray RA, Brennan RG. Structural basis for cooperative DNA binding by two dimers of the multidrug-binding protein QacR. EMBO J 21(5):1210-8, 2002. PMID: 11867549.
31. Godsey MH, Zheleznova Heldwein EE, Brennan RG. Structural biology of bacterial multidrug resistance gene regulators. J Biol Chem 277(43):40169-72, 2002. PMID: 12186881.
32. Schumacher MA, Brennan RG. Structural mechanisms of multidrug recognition and regulation by bacterial multidrug transcription factors. Mol Microbiol 45(4):885-93, 2002. PMID: 12180910.
33. Schumacher MA, Pearson RF, Møller T, Valentin-Hansen P, Brennan RG. Structures of the pleiotropic translational regulator Hfq and an Hfq-RNA complex: a bacterial Sm-like protein. EMBO J 21(13):3546-56, 2002. PMID: 12093755.
34. Schumacher MA, Bashor CJ, Song MH, Otsu K, Zhu S, Parry RJ, Ullman B, Brennan RG. The structural mechanism of GTP stabilized oligomerization and catalytic activation of the Toxoplasma gondii uracil phosphoribosyltransferase. Proc Natl Acad Sci U S A 99(1):78-83, 2002. PMID: 11773618.
35. Heldwein EE, Brennan RG. Crystal structure of the transcription activator BmrR bound to DNA and a drug. Nature 409(6818):378-382, 1/2001. PMID: 11201751.
36. Heldwein EE, Brennan RG. crystal structure of the transcription activator BmrR bound to DNA and a drug. Nature 409:378-382, 1/2001.
37. Craig JC, Schumacher MA, Mansoor SE, Farrens DL, Brennan RG, Goodman RH. Consensus and variant cAMP-regulated enhancers have distinct CREB-binding properties. J Biol Chem 276(15):11719-28, 2001. PMID: 11134034.
38. Godsey MH, Baranova NN, Neyfakh AA, Brennan RG. Crystal structure of MtaN, a global multidrug transporter gene activator. J Biol Chem 276(50):47178-84, 2001. PMID: 11581256.
39. Schumacher MA, Hurlburt BK, Brennan RG. Crystal structures of SarA, a pleiotropic regulator of virulence genes in S. aureus. Nature 409(6817):215-9, 2001. PMID: 11196648.
40. Schumacher MA, Miller MC, Grkovic S, Brown MH, Skurray RA, Brennan RG. Structural mechanisms of QacR induction and multidrug recognition. Science 294(5549):2158-63, 2001. PMID: 11739955.
41. Huffman JL, Mokashi A, Bächinger HP, Brennan RG. The basic helix-loop-helix domain of the aryl hydrocarbon receptor nuclear transporter (ARNT) can oligomerize and bind E-box DNA specifically. J Biol Chem 276(44):40537-44, 2001. PMID: 11502749.
42. Grkovic S, Brown MH, Schumacher MA, Brennan RG, Skurray RA. The staphylococcal QacR multidrug regulator binds a correctly spaced operator as a pair of dimers. J Bacteriol 183(24):7102-9, 2001. PMID: 11717268.
43. Zheleznova EE, Markham P, Edgar R, Bibi E, Neyfakh AA, Brennan RG. A structure-based mechanism for drug binding by multidrug transporters. Trends Biochem Sci 25(2):39-43, 2000. PMID: 10664577.
44. Zheleznova EE, Crosa JH, Brennan RG. Characterization of the DNA- and metal-binding properties of Vibrio anguillarum fur reveals conservation of a structural Zn(2+) ion. J Bacteriol 21(21):6264-7, 2000. PMID: 11029454.
45. Schumacher MA, Scott DM, Mathews II, Ealick SE, Roos DS, Ullman B, Brennan RG. Crystal structures of Toxoplasma gondii adenosine kinase reveal a novel catalytic mechanism and prodrug binding. J Mol Biol 296(2):549-67, 2000. PMID: 10669608.
46. Schumacher MA, Scott DM, Mathews II, Ealick SE, Roos DS, Ullman B, Brennan RG. Crystal structures of Toxoplasma gondii adenosine kinase reveal a novel catalytic mechanism and prodrug binding. J Mol Biol 296:549-67, 2000. PMID: 10669608.
47. Godsey MH, Baranova NN, Neyfakh AA, Brennan RG. Crystallization and preliminary X-ray diffraction studies on the DNA-binding domain of the multidrug transporter activation protein (MtaN) from Bacillus subtilis. Acta Crystallogr D Biol Crystallogr 56(Pt 11):1456-8, 2000. PMID: 11053850.
48. Cardinaux JR, Notis JC, Zhang Q, Vo N, Craig JC, Fass DM, Brennan RG, Goodman RH. Recruitment of CREB binding protein is sufficient for CREB-mediated gene activation. Mol Cell Biol 20(5):1546-52, 2000. PMID: 10669732.
49. Brown M, Schumacher MA, Wiens GD, Brennan RG, Rittenberg MB. The structural basis of repertoire shift in an immune response to phosphocholine. J Exp Med 191(12):2101-12, 2000. PMID: 10859335.
50. Schumacher MA, Goodman RH, Brennan RG. The structure of a CREB bZIP.somatostatin CRE complex reveals the basis for selective dimerization and divalent cation-enhanced DNA binding. J Biol Chem 275(45):35242-7, 2000. PMID: 10952992.
51. Rechtin TM, Gillaspy AF, Schumacher MA, Brennan RG, Smeltzer MS, Hurlburt BK. Characterization of the SarA virulence gene regulator of Staphylococcus aureus. Mol Microbiol 33(2):307-16, 1999. PMID: 10411747.
52. Phillips CL, Ullman B, Brennan RG, Hill CP. Crystal structures of adenine phosphoribosyltransferase from Leishmania donovani. EMBO J 18(13):3533-45, 1999. PMID: 10393170.
53. Zheleznova EE, Markham PN, Neyfakh AA, Brennan RG. Structural basis of multidrug recognition by BmrR, a transcription activator of a multidrug transporter. Cell 96(3):353-62, 1999. PMID: 10025401.
54. Glasfeld A, Koehler AN, Schumacher MA, Brennan RG. The role of lysine 55 in determining the specificity of the purine repressor for its operators through minor groove interactions. J Mol Biol 291(2):347-61, 1999. PMID: 10438625.
55. Lu F, Brennan RG, Zalkin H. Escherichia coli purine repressor: key residues for the allosteric transition between active and inactive conformations and for interdomain signaling. Biochemistry 37(45):15680-90, 1998. PMID: 9843372.
56. Lu F, Schumacher MA, Arvidson DN, Haldimann A, Wanner BL, Zalkin H, Brennan RG. Structure-based redesign of corepressor specificity of the Escherichia coli purine repressor by substitution of residue 190. Biochemistry 37(4):971-82, 1998. PMID: 9454587.
57. Lundblad JR, Kwok RP, Laurance ME, Huang MS, Richards JP, Brennan RG, Goodman RH. The human T-cell leukemia virus-1 transcriptional activator Tax enhances cAMP-responsive element-binding protein (CREB) binding activity through interactions with the DNA minor groove. J Biol Chem 273(30):19251-9, 1998. PMID: 9668114.
58. Arvidson DN, Lu F, Faber C, Zalkin H, Brennan RG. The structure of PurR mutant L54M shows an alternative route to DNA kinking. Nat Struct Biol 5(6):436-41, 1998. PMID: 9628480.
59. Schumacher MA, Zheleznova EE, Poundstone KS, Kluger R, Jones RT, Brennan RG. Allosteric intermediates indicate R2 is the liganded hemoglobin end state. Proc Natl Acad Sci U S A 94(15):7841-4, 1997. PMID: 9223274.
60. Zheleznova EE, Markham PN, Neyfakh AA, Brennan RG. Preliminary structural studies on the multi-ligand-binding domain of the transcription activator, BmrR, from Bacillus subtilis. Protein Sci 6(11):2465-8, 1997. PMID: 9385651.
61. Schumacher MA, Glasfeld A, Zalkin H, Brennan RG. The X-ray structure of the PurR-guanine-purF operator complex reveals the contributions of complementary electrostatic surfaces and a water-mediated hydrogen bond to corepressor specificity and binding affinity. J Biol Chem 272(36):22648-53, 1997. PMID: 9278422.
62. Glasfeld, A, Schumacher, MA, Choi, K-Y, Zalkin, H, Brennan, RG. A Positively Charged Residue Bound in the Minor Groove Does Not Alter the Bending of a DNA Duplex. J. Amer. Chem. Soc 118:13073-13074, 1996.
63. Richards JP, Bächinger HP, Goodman RH, Brennan RG. Analysis of the structural properties of cAMP-responsive element-binding protein (CREB) and phosphorylated CREB. J Biol Chem 271(23):13716-23, 1996. PMID: 8662719.
64. Lewis M, Chang G, Horton NC, Kercher MA, Pace HC, Schumacher MA, Brennan RG, Lu P. Crystal structure of the lactose operon repressor and its complexes with DNA and inducer. Science 271(5253):1247-54, 1996. PMID: 8638105.
65. Schumacher MA, Carter D, Ross DS, Ullman B, Brennan RG. Crystal structures of Toxoplasma gondii HGXPRTase reveal the catalytic role of a long flexible loop. Nat Struct Biol 3(10):881-7, 1996. PMID: 8836106.
66. Phillíps CL, Ullman B, Brennan RG. Crystallization of the purine salvage enzyme adenine phosphoribosyltransferase. Proteins 25(4):510-3, 1996. PMID: 8865346.
67. Schumacher MA, Dixon MM, Kluger R, Jones RT, Brennan RG. Allosteric transition intermediates modelled by crosslinked haemoglobins. Nature 374(6526):84-7, 1995. PMID: 7723849.
68. Nagadoi A, Nakazawa K, Morikawa S, Nakamura H, Sampei G, Mizobuchi K, Yamamoto H, Schumacher MA, Brennan RG, Nishimura Y. Conformational changes of purine repressor DNA-binding domain upon complexation with DNA. Nucleic Acids Symp Ser(34):63-4, 1995. PMID: 8841553.
69. Schumacher MA, Choi KY, Lu F, Zalkin H, Brennan RG. Mechanism of corepressor-mediated specific DNA binding by the purine repressor. Cell 83(1):147-55, 1995. PMID: 7553867.
70. Nagadoi A, Morikawa S, Nakamura H, Enari M, Kobayashi K, Yamamoto H, Sampei G, Mizobuchi K, Schumacher MA, Brennan RG. Structural comparison of the free and DNA-bound forms of the purine repressor DNA-binding domain. Structure 3(11):1217-24, 1995. PMID: 8591032.
71. Schumacher MA, Choi KY, Zalkin H, Brennan RG. Crystal structure of LacI member, PurR, bound to DNA: minor groove binding by alpha helices. Science 266(5186):763-70, 1994. PMID: 7973627.
72. Schumacher MA, Choi KY, Zalkin H, Brennan RG. Crystallization and preliminary X-ray analysis of an Escherichia coli purine repressor-hypoxanthine-DNA complex. J Mol Biol 242(3):302-5, 1994. PMID: 8089849.
73. Loriaux MM, Brennan RG, Goodman RH. Modulatory function of CREB.CREM alpha heterodimers depends upon CREM alpha phosphorylation. J Biol Chem 269(46):28839-43, 1994. PMID: 7961842.
74. Brickey DA, Bann JG, Fong YL, Perrino L, Brennan RG, Soderling TR. Mutational analysis of the autoinhibitory domain of calmodulin kinase II. J Biol Chem 269(46):29047-54, 1994. PMID: 7961870.
75. Kwok RP, Lundblad JR, Chrivia JC, Richards JP, Bächinger HP, Brennan RG, Roberts SG, Green MR, Goodman RH. Nuclear protein CBP is a coactivator for the transcription factor CREB. Nature 370(6486):223-6, 1994. PMID: 7913207.
76. Loriaux MM, Rehfuss RP, Brennan RG, Goodman RH. Engineered leucine zippers show that hemiphosphorylated CREB complexes are transcriptionally active. Proc Natl Acad Sci U S A 90(19):9046-50, 1993. PMID: 8105470.
77. Spiro C, Richards JP, Chandrasekaran S, Brennan RG, McMurray CT. Secondary structure creates mismatched base pairs required for high-affinity binding of cAMP response element-binding protein to the human enkephalin enhancer. Proc Natl Acad Sci U S A 90(10):4606-10, 1993. PMID: 8506306.
78. Schumacher MA, Macdonald JR, Björkman J, Mowbray SL, Brennan RG. Structural analysis of the purine repressor, an Escherichia coli DNA-binding protein. J Biol Chem 268(17):12282-8, 1993. PMID: 8509365.
79. Schumacher MA, Choi KY, Zalkin H, Brennan RG. Crystallization and preliminary X-ray studies on the co-repressor binding domain of the Escherichia coli purine repressor. J Mol Biol 225(4):1131-3, 1992. PMID: 1613795.
80. Wilson KP, Shewchuk LM, Brennan RG, Otsuka AJ, Matthews BW. Escherichia coli biotin holoenzyme synthetase/bio repressor crystal structure delineates the biotin- and DNA-binding domains. Proc Natl Acad Sci U S A 89(19):9257-61, 1992. PMID: 1409631.
81. Carr DW, Stofko-Hahn RE, Fraser ID, Bishop SM, Acott TS, Brennan RG, Scott JD. Interaction of the regulatory subunit (RII) of cAMP-dependent protein kinase with RII-anchoring proteins occurs through an amphipathic helix binding motif. J Biol Chem 266(22):14188-92, 1991. PMID: 1860836.
82. Dixon MM, Brennan RG, Matthews BW. Structure of gamma-chymotrypsin in the range pH 2.0 to pH 10.5 suggests that gamma-chymotrypsin is a covalent acyl-enzyme adduct at low pH. Int J Biol Macromol 13(2):89-96, 1991. PMID: 1888717.
83. Thomas KW, Kelly AP, Beers PT, Brennan RG. Thiamine deficiency in sheep exported live by sea. Aust Vet J 67(6):215-8, 6/1990. PMID: 2222365.
84. Brennan RG, Roderick SL, Takeda Y, Matthews BW. Protein-DNA conformational changes in the crystal structure of a lambda Cro-operator complex. Proc Natl Acad Sci U S A 87(20):8165-9, 1990. PMID: 2146682.
85. Brennan RG, Vasu S, Matthews BW, Otsuka AJ. Crystallization of the bifunctional biotin operon repressor. J Biol Chem 264(1):5, 1989. PMID: 2642476.
86. Brennan, RG, Wozniak, J, Faber, R, Matthews, BW. Crystallization of Mutant Lysozymes from Bacteriophage T4. . J. Crystal Growth 90:160-167, 1988.
87. Brennan RG, Pyzalska D, Blonski WJ, Hruska FE, Sundaralingam M. Crystal structure of the promutagen O4-methylthymidine: importance of the anti conformation of the O(4) methoxy group and possible mispairing of O4-methylthymidine with guanine. Biochemistry 25(5):1181-5, 1986. PMID: 3964665.
88. Brennan RG, Takeda Y, Kim J, Anderson WF, Matthews BW. Crystallization of a complex of cro repressor with a 17 base-pair operator. J Mol Biol 188(1):115-8, 1986. PMID: 2940371.
89. Brennan RG, Westhof E, Sundaralingam M. Structure of a Z-DNA with two different backbone chain conformations. Stabilization of the decadeoxyoligonucleotide d(CGTACGTACG) by [Co(NH3)6]3+ binding to the guanine. J Biomol Struct Dyn 3(4):649-65, 1986. PMID: 3271042.
90. Brennan, RG, Kim, J, Takeda, Y, Anderson, WF, Matthews, BW. Studies on the Crystallization of the Cro Protein-Pseudo OR3 Complex. J. Crystal Growth 76:715-718, 1986.
91. Brennan, RG, Weaver, LH, Matthews, BW. Use of Protein Sequence and Structure to Infer Distant Evolutionary Relationships. Symposium on the Molecular Evolution of Life, Södergarn", Chemica Scripta 26B:1986, 1986.
92. Brennan RG, Sundaralingam M. Crystallization and preliminary crystallographic studies of the decadeoxyoligonucleotide d(CpGpTpApCpGpTpApCpG). J Mol Biol 181(4):561-3, 1985. PMID: 3999140.
93. Brennan RG, Kondo NS, Sundaralingam M. Crystal Structure and Conformation of the Phosphotriester Adenosine-5'-O-Diethylphosphate. Possible Steric and Conformational Mechanisms for the Biochemical and Biological Effects Arising from Phosphate Alkylation. J. Am. Chem. Soc 106:5671-5676, 1984.
94. Brennan RG, Kondo NS, Sundaralingam M. X-ray structure of cytidine-5'-O-dimethylphosphate. Novel stacking between the ribosyl O(2') hydroxyl oxygen atom and the base. Nucleic Acids Res 12(17):6813-25, 1984. PMID: 6548306.
95. Brennan RG, Privé GG, Blonski WJ, Hruska FE, Sundaralingam M. Crystal structure of O4-methyl uridine: stacking induced changes in the geometry of the pyrimidine ring and its mutagenic role. J Biomol Struct Dyn 1(4):939-45, 1983. PMID: 6400906.
96. Brennan RG, Privé GG, Blonski WJP, Hruska FE, Sundaralingam M. Molecular and Crystal Structure of O4Methyluridine. Reaction Coordinates for an Incipient Nucleophilic Attack Seen by Short Intermolecular Sugar-Base Contacts. J. Am. Chem. Soc 105:7737-7742, 1983.
97. Brennan RG, Sundaralingam M, BeMiller JN. The X-ray Structure of 3,4,5,7-Tetra- O-Acety1-2,6-Anhydro-D-Glycero-D-TaloHeptonamide. J. Carbohyd. Chem 2:115-128, 1983.
98. Sundaralingam M, Brennan RG, Swaminathan P, Haromy TP, Drendel WB, BeMiller JN, Chmielewski M and Cerretti DP. Chair Conformation in Two Carbohydrates and the Reverse Anomeric Effect. X-ray Structures of 3,4,5,7-Tera-O-Acety 1-2, 5-Anhydro-D-Glycero-D-Ido-Heptonamide (1) and 3,4,5,7-Tetra-O-Aceyty 1-2,6-Anhydro-D-Glycero-D-Gluco-Heptonamide (2). J. Carbohyd. Chem. 1:85-103, 1982.
99. Holtz A, Brennan RG, Battista D, Terner C. Androgen Control of an Inhibitory Modulator of Phosphodiesterase in Rat Epididymis and Prostate. Endocrinology 108:1538-1544, 1981.

Invited Articles
1. Ramos, JL, Martínez-Beuno, M, Molina-Herares, AJ, Terán, W, Watanabe, K, Zhang, X, Trinidad Gallegos, Brennan, R, Tobes, R. The TetR Family of Transcriptional Repressors. Microbiol. Molec. Biol. Rev 69:326-356, 2005.
2. Schumacher MA, Brennan RG. Deciphering the molecular basis of multidrug recognition: crystal structures of the Staphylococcus aureus multidrug binding transcription regulator QacR. Res Microbiol 154(2):69-77, 2003. PMID: 12648720.
3. Huffman JL, Brennan RG. Prokaryotic transcription regulators: more than just the helix-turn-helix motif. Curr Opin Struct Biol 12(1):98-106, 2002. PMID: 11839496.
4. Brennan RG, Guest Editor. Multidrug Resistance. Seminars in Cell and Developmental Biology 12:1-4, 2001.
5. Vazquez-Laslop N, Zheleznova EE, Markham PN, Brennan RG, Neyfakh AA. Recognition of multiple drugs by a single protein: a trivial solution of an old paradox. Biochem Soc Trans 28(4):517-20, 2000. PMID: 10961951.
6. Brennan RG. The winged-helix DNA-binding motif: another helix-turn-helix takeoff. Cell 74(5):773-6, 1993. PMID: 8374950.
7. Brennan RG. DNA Recognition by the Helix-Turn-Helix Motif. Curr. Opin. Struct. Biol 2:100-108, 1992.
8. Brennan RG. Interactions of the Helix-Turn-Helix Binding Domain. Curr. Opin. Struct. Biol 1:80-88, 1991.
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Last updated: 6/23/2009