Keigi Fujiwara, PhD
Department of Cardiology, Division of Internal Medicine
About Dr. Fujiwara
I received my PhD in Biology from the University of Pennsylvania and postdoctoral training at the Department of Cell Biology and Anatomy at Harvard Medical School under Dr. Thomas Pollard. After my postdoctoral training, I was appointed an Assistant Professor and then Associate Professor. I helped create the Department of Structural Analysis with the newly founded National Cardiovascular Center Research Institute in Japan. I served as chairman of the department for 16 years. Afterwards, I returned to the US as a Professor in the Department of Medicine at Rochester University Medical School. In 2014, I joined MD Anderson as a Professor in the Department of Cardiology.
Present Title & Affiliation
Primary Appointment
Professor, Department of Cardiology - Research, Division of Internal Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX
Research Interests
I am a cell biologist interested in cell motility such as cell migration, cell division, intracellular organelle trafficking, and cell contraction. Our studies on the cytoskeleton of endothelial cells have led us to the field of endothelial mechanotransduction. While many investigators are studying various responses, such as changes in the gene and protein expression, morphological changes, activation of signaling cascades, and so on, of cells exposed to mechanical forces, we focus onto mechanosensing. How do cells sense mechanical forces? Through a series of in vitro cell and molecular biological studies, we were able to identify platelet endothelial cell adhesion molecule 1 (PECAM-1/CD31) to be a mechanosensor molecule of endothelial cells. The precise mechanism for mechanotransduction by PECAM-1 is being investigated. Our studies are also aimed at endothelial cell biology and physiology, especially on topics related to the cytoskeleton and cell adhesion and to cell signaling in the context of atherogenesis, endothelial dysfunction, and cancer treatment-induced cardiovascular events. These latter studies are conducted in collaboration with Dr. Jun-ichi Abe, Professor, UT MD Anderson Cancer Center. In addition, we are investigating how to mitigate effects of space radiation and microgravity which are known risk factors for astronauts to develop cancer and other diseases including neurological, cardiovascular and general systemic dysfunction. As a graduate student in the Biophysical Cytology Program at Penn headed by Dr. Shinya Inoue, I received arguably the best training on light microscopy. Throughout my scientific career, I have given numerous numbers of workshops, courses, and lectures on microscopy, especially fluorescence microscopy and live cell imaging techniques using confocal and multi-photon microscopy, FRET, TIRF, intravital microscopy, polarization microscopy, and time lapse recording. More recently, we employ Imaging Mass Cytometry (IMC) to study expression of >30 proteins simultaneously within cells in tissues.
Education & Training
Degree-Granting Education
| 1974 | University of Pennsylvania, Phildelphia, PA, USA, PHD, Biology, Program in Biophysical Cytology |
| 1968 | International Christian University, Mitaka, JPN, BA, Natural Sciences; Division of Biology |
Postgraduate Training
| 1974-1977 | Research Fellowship, Harvard Medical School, Department of Anatomy, Boston, MA |
| 1973-1973 | Physiology Course, Marine Biological Laboratory, Wood Holes, MA |
Experience & Service
Academic Appointments
Research Professor, Department of Department of Medicine, Division of Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, NY, 2007 - 2014
Research Professor, Department of Department of Biomedical Engineering, Division of School of Engineering, University of Rochester, Rochester, NY, 2007 - 2014
Professor, Department of Department of Biomedical Engineering, Division of School of Engineering, University of Rochester, Rochester, NY, 2006 - 2007
Professor, Department of Department of Medicine, Division of Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, Rochester, NY, 2001 - 2007
Administrative Appointments/Responsibilities
Co-Director, Department of Department of Medicine, University of Rochester, Rochester, NY, 2013 - 2014
Director, Department of Aab Cardiovascular Research Institute, University of Rochester, Rochester, NY, 2005 - 2014
Other Appointments/Responsibilities
Microscopy Core Committee Member, University of Rochester, Rochester, NY, 2010 - 2014
Annotator, The Cell: An Imaging Library, American Society for Cell Biology, Bethesda, MD, 2010 - 2012
Electron Microscope Core Committee Member, University of Rochester, Rochester, NY, 2006 - 2014
Honors & Awards
| 1978 | Steps-Toward-Independence Fellowship, Marine Biological Laboratory |
| 1975 | Muscular Dystrophy Association Postdoctoral Fellowship, Muscular Dystrophy Association |
| 1962 | American Field Service (AFS) International Scholarship, American Field Service (AFS) |
Professional Memberships
Selected Publications
Peer-Reviewed Articles
- Abe R, Savage H, Imanishi M, Banarjee P, Kotla S, Paez-Mayorga, Taunton J, Fujiwara K, Won JH, Wamique Y, Palaskas NL, Banchs J, Lin SH, Schadler KL, Abe J, Le NT. P90RSK-MAGI1 modul controls endothelia permeability by post-translational modifications of MAGI1 and Hippo pathway. Frontiers in Cardiovascu. Medicine 7(542485), 2020.
- Yamanouchi S, Rhone J, Mao JH, Fujiwara K, Saganti PB, Takahashi A, Hada M. Simultaneous Exposure of Cultured Human Lymphoblastic Cells to Simulated Microgravity and Radiation Increases Chromosome Aberrations. Life (Basel) 10(9), 2020. e-Pub 2020. PMID: 32927618.
- McBeath E, Parker-Thornburg J, Fujii Y, Aryal N, Smith C, Hofmann MC, Abe JI, Fujiwara K. Rapid Evaluation of CRISPR Guides and Donors for Engineering Mice. Genes (Basel) 11(6), 2020. e-Pub 2020. PMID: 32521708.
- Luo W, Wang Y, Zhang L, Ren P, Zhang C, Li Y, Azares AR, Zhang M, Guo J, Ghaghada KB, Starosolski ZA, Rajapakshe K, Coarfa C, Li Y, Chen R, Fujiwara K, Abe JI, Coselli JS, Milewicz DM, LeMaire SA, Shen YH. Critical Role of Cytosolic DNA and Its Sensing Adaptor STING in Aortic Degeneration, Dissection, and Rupture. Circulation 141(1):42-66, 2020. e-Pub 2019. PMID: 31887080.
- Kotla S, Le NT, Vu HT, Ko KA, Gi YJ, Thomas TN, Giancursio C, Lusis AJ, Cooke JP, Fujiwara K, Abe JI. Endothelial senescence-associated secretory phenotype (SASP) is regulated by Makorin-1 ubiquitin E3 ligase. Metabolism 100:153962, 2019. e-Pub 2019. PMID: 31476350.
- Kotla S, Vu HT, Ko KA, Wang Y, Imanishi M, Heo KS, Fujii Y, Thomas TN, Gi YJ, Mazhar H, Paez-Mayorga J, Shin JH, Tao Y, Giancursio CJ, Medina JL, Taunton J, Lusis AJ, Cooke JP, Fujiwara K, Le NT, Abe JI. Endothelial senescence is induced by phosphorylation and nuclear export of telomeric repeat binding factor 2-interacting protein. JCI Insight 4(9), 2019. e-Pub 2019. PMID: 31045573.
- Abe JI, Ko KA, Kotla S, Wang Y, Paez-Mayorga J, Shin IJ, Imanishi M, Vu HT, Tao Y, Leiva-Juarez MM, Thomas TN, Medina JL, Won JH, Fujii Y, Giancursio CJ, McBeath E, Shin JH, Guzman L, Abe RJ, Taunton J, Mochizuki N, Faubion W, Cooke JP, Fujiwara K, Evans SE, Le NT. MAGI1 as a link between endothelial activation and ER stress drives atherosclerosis. JCI Insight 4(7), 2019. e-Pub 2019. PMID: 30944250.
- Moslehi J, Fujiwara K, Guzik T. Cardio-oncology: a novel platform for basic and translational cardiovascular investigation driven by clinical need. Cardiovasc Res 115(5):819-823, 2019. PMID: 30888396.
- Singh MV, Kotla S, Le NT, Ae Ko K, Heo KS, Wang Y, Fujii Y, Thi Vu H, McBeath E, Thomas TN, Jin Gi Y, Tao Y, Medina JL, Taunton J, Carson N, Dogra V, Doyley MM, Tyrell A, Lu W, Qiu X, Stirpe NE, Gates KJ, Hurley C, Fujiwara K, Maggirwar SB, Schifitto G, Abe JI. Senescent Phenotype Induced by p90RSK-NRF2 Signaling Sensitizes Monocytes and Macrophages to Oxidative Stress in HIV-Positive Individuals. Circulation 139(9):1199-1216, 2019. PMID: 30586719.
- Ikeda H, Muratani M, Hidema J, Hada M, Fujiwara K, Souda H, Yoshida Y, Takahashi A.. Expression Profile of Cell Cycle-related Genes in Human Fibroblasts Exposed Simulataneously to Radiation and Simulated Microgravity. Int. J. Mol. Sci 20(19):4791, 2019.
- Hada M, Ikeda H, Rhone JR, Beitman AJ, Plante I, Souda H, Yoshida Y, Held KD, Fujiwara K, Saganti PB, Takahashi A. Increased Chromosome Aberrations in Cells Exposed Simultaneously to Simulated Microgravity and Radiation. Int J Mol Sci 20(1), 2018. e-Pub 2018. PMID: 30583489.
- Snyder JL, McBeath E, Thomas TN, Chiu YJ, Clark RL, Fujiwara K. Mechanotransduction properties of the cytoplasmic tail of PECAM-1. Biol Cell 109(8):312-321, 2017. e-Pub 2017. PMID: 28643869.
- Lin SC, Lee YC, Yu G, Cheng CJ, Zhou X, Chu K, Murshed M, Le NT, Baseler L, Abe JI, Fujiwara K, deCrombrugghe B, Logothetis CJ, Gallick GE, Yu-Lee LY, Maity SN, Lin SH. Endothelial-to-Osteoblast Conversion Generates Osteoblastic Metastasis of Prostate Cancer. Dev Cell 41(5):467-480.e3, 2017. PMID: 28586644.
- Ko KA, Fujiwara K, Krishnan S, Abe JI. En face preparation of mouse blood vessels. J Vis Exp(123), 2017. e-Pub 2017. PMID: 28570508.
- Mao Y, Luo W, Zhang L, Wu W, Yuan L, Xu H, Song J, Fujiwara K, Abe JI, LeMaire SA, Wang XL, Shen YH. STING-IRF3 Triggers Endothelial Inflammation in Response to Free Fatty Acid-Induced Mitochondrial Damage in Diet-Induced Obesity. Arterioscler Thromb Vasc Biol 37(5):920-929, 2017. e-Pub 2017. PMID: 28302626.
- Mao Y, Luo W, Zhang L, Wu W, Yuan L, Xu H, Fujiwara K, Abe J, Wang XL, and Shen YH.. STING-IRF3 triggers endothelial inflammation in response to free fatty acid-induced mitochondrial damage. Arterioscler Thromb Vasc Biol, 2017.
- Abe JI, Sandhu UG, Hoang NM, Thangam M, Quintana-Quezada RA, Fujiwara K, Le NT. Coordination of Cellular Localization-Dependent Effects of Sumoylation in Regulating Cardiovascular and Neurological Diseases. Adv Exp Med Biol 963:337-358, 2017. PMID: 28197922.
- Xu S, Ha CH, Wang W, Xu X, Yin M, Jin FQ, Mastrangelo M, Koroleva M, Fujiwara K, Jin ZG.. PECAM1 regulates flow-mediated Gab1 tyrosine phosphorylation and signaling. Cellular Signaling 28:117-124, 2016.
- Chatterjee S, Fujiwara K, Pérez NG, Ushio-Fukai M, Fisher AB. Mechanosignaling in the vasculature: emerging concepts in sensing, transduction and physiological responses. Am J Physiol Heart Circ Physiol 308(12):H1451–H1462, 2015. e-Pub 2015. PMID: 25862828.
- Heo KS, Le NT, Cushman HJ, Giancursio CJ, Chang E, Woo CH, Sullivan MA, Taunton J, Yeh ETH, Fujiwara K, Abe J.. Disturbed flow-activated p90RSK kinase accelerates atherosclerosis by inhibiting SENP2 function. J Clin Invest 125(3):1299-1310, 2015.
- Heo KS, Cushman HJ, Akaike M, Woo CH, Wang X, Qiu X, Fujiwara K, Abe J. ERK5 activation in macrophages promotes efferocytosis and inhibits atherosclerosis. Circulation 130(2):180-91, 2014. e-Pub 2014. PMID: 25001623.
- Le NT, Heo KS, Takei Y, Lee H, Woo CH, Chang E, McClain C, Hurley C, Wang X, Li F, Xu H, Morrell C, Sullivan MA, Cohen MS, Serafimova IM, Taunton J, Fujiwara K, Abe J. A crucial role for p90RSK-mediated reduction of ERK5 transcriptional activity in endothelial dysfunction and atherosclerosis. Circulation 127(4):486-99, 2013. e-Pub 2012. PMID: 23243209.
- Kim GY, Nigro P, Fujiwara K, Abe J, Berk BC. p62 binding to protein kinase C ζ regulates tumor necrosis factor α-induced apoptotic pathway in endothelial cells. Arterioscler Thromb Vasc Biol 32(12):2974-80, 2012. e-Pub 2012. PMID: 23023376.
- Le NT, Takei Y, Shishido T, Woo CH, Chang E, Heo KS, Lee H, Lu Y, Morrell C, Oikawa M, McClain C, Wang X, Tournier C, Molina CA, Taunton J, Yan C, Fujiwara K, Patterson C, Yang J, Abe J. p90RSK targets the ERK5-CHIP ubiquitin E3 ligase activity in diabetic hearts and promotes cardiac apoptosis and dysfunction. Circ Res 110(4):536-50, 2012. e-Pub 2012. PMID: 22267842.
- Wang XQ, Nigro P, World C, Fujiwara K, Yan C, Berk BC. Thioredoxin interacting protein promotes endothelial cell inflammation in response to disturbed flow by increasing leukocyte adhesion and repressing Kruppel-like factor 2. Circ Res 110(4):560-8, 2012. e-Pub 2012. PMID: 22267843.
- Moudgil R. Ko KA, VuHT, Thomas NT, Wang J, Chang J, Reddy AK, Fujiwara K, Abe J.. Topoisomerase 2B decrease results in diastolic dysfunction via 953 and Akt: A novel pathway. J. AM. Coll. Cardiol.
Invited Articles
- Le NT, Martin JF, Fujiwara K, Abe JI. Sub-cellular localization specific SUMOylation in the heart. Biochim Biophys Acta Mol Basis Dis 1863(8):2041-2055, 2017. e-Pub 2017. PMID: 28130202.
- Le NT, Sandhu UG, Quintana-Quezada RA, Hoang NM, Fujiwara K, Abe JI. Flow signaling and atherosclerosis. Cell Mol Life Sci 74(10):1835-1858, 2017. e-Pub 2016. PMID: 28039525.
- Le NT, Abe J, Hoang NH Fujiwara. MEK5/ERK5. Encyclopedia of Signaling Molecules 2nd Edition, 2016.
Editorials
- Moslehi J, Fujiwarta K, Guzik, T.. Cardio-oncology: A novel platform for basic and tranlational cardiovascular investigation driven by clinical need. Cardiovascular 115:819-823, 2019.
- Fujiwara K, Sheu SS. Mitochondrial dynamics regulate neointima formation. Cardiovasc Res 106(2):175-7, 2015. PMID: 25800033.
Abstracts
- Takahashi A, Ikeda H, Muratani M, Hidema J, Hada M, Fujiwara K, Souda H, Yoshida Y. Expression profile of cell cycle-related genes in human fibroblasts exposed simultaneously to radiation and simulated microgravity. The 35th Annual Meeting for the American Society for Gravitational and Space Research, Denver, CO, 2019.
- Hada M, Rhone JR, Beitman A, Ikeda H, Plante I, Souda H, Yoshida Y, Held KD, Fujiwara K, Saganti PB, Takahashi A. Increased Chromosome Aberrations in cells exposed simultaneously to simulated microgravity and radiation. The 35th Annual Meeting for the American Society for Gravitational and Space Research, Denver, CO, 2019.
- Fujiwara K, Hada M, Takahashi A. Gravity sensing by cells and effects of simultaneous exposure of cells to microgravity and irradiation on chromosomes. 9th Annual world Congress of Molecular and Cell Biology-2019. Singapore62. Fujiwara K, Hada M, Takahashi A, 2019.
- Fujiwara K, Hada M, Takahashi A. Gravity sensing by cells and effects of simultaneous exposure of cells to microgravity and irradiation on chromosomes. Molecular and Cell Biology, Singapore, 2019.
- Takahashi A, Ikeda H, Muratani M, Hidema J, Hada M, Fujiwara K, Souda H, Yoshida Y. Combined effects of radiation and microgravity: RNAseq analysis. The 33rd Annual Meeting of Japanese Society for Biological Science in Space, Chiba, Japan, 2019.
- Chiu YJ. Thomas TN, Choe LH, Lee KH, Fujiwara K. The early response of cultured mammalian cells to changes in gravity: increased actin dynamics. The 2nd Mechanobiology Meeting. ICISE, Qui Nhon, Vietnam, 2019.
- Kotla S, Zhang A, Ko KA, Imanishi M, Mazhar H, Thomas TN, Gi YJ, Vu HT, McBeath E, Lin SH, Palaskas NL, Banchs J, Kleinerman ES, Schadler KL, Fujiwara K, Le NT, Hamilton D, Krishnan S, Abe J. Cardiovascular toxic cancer treatments (CTCT) prime monocyte and macrophages to oxidative stress and accelerate atherogenesis. ATVB, Boston, MA, 2019.
- Imanishi M, Ko KA, Wang Y, Heo KS, Fujii Y, Thomas TN, Gi YJ, Mazhar H, Fujiwara K, Le NT, Kotla S, and Abe J. Disturbed Flow-induced Endothelial Senescence, But Not Inflammation, Plays A Major Role In Plaque Formation Mediated By Telomeric Repeat Binding Factor 2-interacting Protein (TERF2IP) K240 SUMOylation. ATVB, 2019.
- 6Vu HT, Heo KS, Imanishi M, Ko KA, Thomas TN, Gi YJ, Mazhar H, Fujiwara K, Le NT, Abe J, and Kotla S. The key role of endothelial SUMOylation in regulating sustained NADPH oxidase activation under disturbed flow: Involvement of feedback loop formed by FAK-SUMOylation. ATVB, Boston, MA, 2019.
- McBeath E, Fujii Y, Cruz L, Abe J, Hofmann M, Fujiwara K. SED (single-strand-ended double strand) DNA donors as an inexpensive, easy to make, and efficiently inserted, long CRISPR donor. 15th Transgenic Technology Meeting, Kobe, Japan, 2019.
- Rhone JR, Beitman A, Ikeda H, Plante I, Souda H, Y. Yoshida4, Held KD, Fujiwara K, Takahashi A, Saganti PB, and Hada M. Increased chromosome aberrations in cells exposed simultaneously to simulated microgravity and radiation. 2019 NASA Human Research Program Investigators’ Workshop, Galveston, TX, 2019.
- McBeath E, Parker-Thornburg J, Fujii Y, Smith C, Hofmann M, Abe J, Fujiwara K. Rapidly increasing knock-in efficiency of CRISPR in mice by using blastocysts as a testing model. American Society for Cell Biology, San Diego, CA, 2018.
- Kotla S, Singh MV, Le NT, Ko KA, Heo KS, Fujii Y, Vu HT, McBeath E, Thomas TN, Tao Y, Medina J, Stirpe NE, Lu W, Tyrell A, Gates KJ, Qui X, Fujiwara K, Maggirwar SJ, Schifitto G, and Abe J. Senescent phenotype induced by p90RSK-NRF2 signaling sensitizes monocytes and macrophages to oxidative stress in HIV+ individuals: implications for atherogenesis. NAVBO, Boston, MA, 2018.
- Fujiwara K, Snyder JL, McBeath E, Thomas TN, Chiu YJ, Clark RL. Mechanism of PECAM-1 mechanotransduction. 8th World Congress of Biomechanics. Dublin, Ireland, 2018.
- Vu HT, Kotla S, Wang Y, Ko KA, Heo KS, Fujii Y, Thomas TN, Gi YJ, Medina J, Fujiwara K, Le NT, and Abe J. Disturb flow induces p90RSK activation in endothelial cell, which increases TERF2IP phosphorylation causing endothelial cell dysfunction. ATVB, 2018.
- Fujii Y, Vu HT, Kolta S, Ko KA, Wang Y, Thomas TN, Heo KS, Le NT, Fujiwara K, and Abe J. Disturbed flow-induced TERF2IP K240 SUMOylation is critical for endothelial senescence but not inflammation. ATVB, 2018.
- Vu HT, Kotla S, Ko KA, Fujii Y, Tao Y, Medina J, Thomas TN, Hada M, Sood AK, Singh PK5, Milgrom SA, Krishnan S, Fujiwara K, Le NT, and Abe J. Ionizing radiation induces endothelial inflammation and apoptosis via p90RSK-mediated ERK5 S496 phosphorylation. ATVB, 2018.
- Kotla S, Le NT, Singh MV, Ko KA, Heo KS, Fujii Y, Vu HT, McBeath E, Thomas TN, Tao Y, Medina J, Stirpe NE, Lu W, Tyrell A, Gates KJ, Qui X, Fujiwara K, Maggirwar SJ, Schifitto G, and Abe J. Monocyte/macrophages are sensitized to secondary oxidative insult by cardiovascular toxic drugs via the p90RSK-ERK5 S496 phosphorylation pathway, aggrandizing atherosclerosis formation. ATVB, 2018.
- Ko KA, Kotla S, Wang Y, Fujii Y, Vu HT, Venkatesulu BP, Thomas TN, Medina J, Gi YJ1, Hada M, Grande-Allen J, Patel ZS, Milgrom SA, Kirshnan S, Fujiwara K,, and Abe J. Radiation induced atherosclerosis on mice. ATVB, 2018.
- Kotla S, Le NT, Singh MV, Ko KA, Heo KS, Fujii Y, Vu HT, McBeath E, Thomas TN, Tao Y, Medina J, Stirpe NE, Lu W, Tyrell A, Gates KJ, Qui X, Fujiwara K, Maggirwar SJ, Schifitto G, and Abe. Monocyte/macrophages are sensitized to secondary oxidative insult by cardiovascular toxic drugs via the p90RSK-ERK5 S496 phosphorylation pathway, aggrandizing atherosclerosis formation. Keystone Symposia, Santa Fe, 2018.
- McBeath E, Snyder JL, Thomas TN, Chiu YJ, Clark RL, Fujiwara K. Mechanotransduction properties of the PECAM-1 cytoplasmic tail. American Society for Cell Biology and EMBO, 2017.
- Le NT, Singh MV, Ko KA, Heo KS, Fujii Y, Vu HT, McBeath E, Thomas TN, Tao Y, Medina J, Stirpe NE, Lu W, Tyrell A, Gates KJ, Qui X, Fujiwara K, Maggirwar SJ, Schifitto G, and Abe J. The Combination Antiretroviral Therapy (cart) Inhibits Efferocytosis-Related Gene Expression via Activating P90rsk and Accelerates Atherosclerosis. Anaheim, 2017.
- Le NT, Heo KS, KO KA, Thomas T, Fujiwara K, and Abe J. TERF2IP S205 phosphorylation mediates atherosclerosis via orchestratingendothelial cell senescence and inflammation. Aging and mechanisms of aging-related disease. Keystone symposium, Yokohama, Japan, 2017.
- McBeath E, Parker-Thornburg J, Smith C, Abe J, Fujiwara K. Use of blastocysts to determine the effectiveness of different sgRNAs and donor DNAs for genomic editing by CRISPR/Cas9 prior to use in mic. American Society for Cell Biology, 2016.
- Le NT, Heo KS, Ko Ka, Thomas T, Fujiwara K, and Abe J. Post-translational modification of telomeric repeat-binding factor 2 (TERF2)-interacting protein (TERF2IP) mediates atherosclesis (AS) via orchestrating endothelial cell (EC) senescence (Sen) and inflammation. American Heart Association Scientific Sessions, 2016.
- Heo KS, Ko KA, Le NT, Fujiwara E, Thomas T, Medina J, Taunton J, Fujiwara K, and Abe J. The combination antiretroviral therapy (cART) inhibited efferocytosis-related gene expression via regulating p90RSK-ERK5 module and accelerated atherosclerosis development. Gulf Coast Vascular Research Consortium, 2016.
- Le NT, Fujiwara K, and Abe J. TRAF2 and NCK-interacting protein kinase (TNIK) as a molecular target of ponatinib- induced endothelial apoptosis and inflammation. Gulf Coast Vascular Research Consortium, 2016.
- Le NT, Won JH, Fujiwara K, and Abe J. Membrane-associated guanylate kinase with inverted domain structure-1 (MAGI1) regulates non-canonical large tumor suppressors (LATSs) kinase signaling and promotes endothelial (EC) apoptosis and inflammation. Gulf Coast Vascular Research Consortium, 2016.
- Shin I, Won JH, Ko KA, Fujiwara E, Shin JH, Thomas T, Taunton J, Hosokawa H, Mochizuki N, Yeh ET, Abe J, Fujiwara K, and Le NT. The Membrane-associated guanylate kinase WW and PDZ Domain-containing Protein 1 (MAGI1) is required for disturbed flow-induced endothelial inflammation and atherosclerosis. 1st ASIA Conference on Pharmaceutical Sciences, 2016.
- Shin I, Won JH, Ko KA, Fujiwara E, Shin JH, Thomas T, Taunton J, Hosokawa H, Mochizuki N, Yeh ET, Abe J, Fujiwara K, and Le NT. The Membrane-associated guanylate kinase WW and PDZ Domain-containing Protein 1 (MAGI1) is required for disturbed flow-induced endothelial inflammation and atherosclerosis. 1st ASIA Conference on Pharmaceutical Sciences, 2016.
- Snyder J, McBeath E, Chiu YJ, Osawa M, Masuda M, Thomas T, Clark R, Fujiwara K. PECAM-1 is a mechanosensor of endothelial cells. Quy Nhon, Vietnam, 2016.
- Abe J, Shin I, Ko KA, Fujiwara E, Shin JH, Thomas T, Fujiwara E, Taunton J, Medina J, Won JH, Fujiwara K, and Le NT. Membrane-associated guanylate kinase with inverted domain structure-1 (MAGI1) as a key pro-inflammatory molecule in disturbed flow-induced atherosclerosis formation. 11th International Symposium on BioMechanics in Vascular Biology and Cardiovascular Disease, 2016.
- Heo KS, Ko KA, Le NT, Fujiwara E, Thomas T, Medina J, Taunton J, Fujiwara K, and Abe J.. The combination antiretroviral therapy (cART) inhibited efferocytosis-related gene expression via regulating p90RSK-ERK5 module and accelerated atherosclerosis development. Division of Internal Medicine, Retreat, 2016.
- Le NT, Shin I, Ko KA, Thomas T, Fujiwara E, Medina J, Won JH, Fujiwara K, and Abe J. Membrane-associated guanylate kinase with inverted domain structure-1 (MAGI1) as a key pro-inflammatory molecule in promoting chronic inflammatory disease. Division of Internal Medicine, Retreat, 2016.
- Le NT, Fujiwara K, and Abe J. TRAF2 and NCK-interacting protein kinase (TNIK) as a molecular target of ponatinib- induced endothelial apoptosis and inflammation. Division of Internal Medicine, Retreat, 2016.
- Le NT, Won JH, Fujiwara K, and Abe J. Membrane-associated guanylate kinase with inverted domain structure-1 (MAGI1) regulates non-canonical large tumor suppressors (LATSs) kinase signaling and promotes endothelial (EC) apoptosis and inflammation. Division of Internal Medicine, Retreat, 2016.
- Shin I, Won JH, Ko KA, Fujiwara E, Shin JH, Thomas T, Taunton J, Hosokawa H, Mochizuki N, Yeh ET, Abe J, Fujiwara K, and Le N. The membrane-associated guanylate kinase WW and PDZ domain-containing protein 1 (MAGI1) is required for disturbed flow-induced endothelial inflammation and atherosclerosis. Keystone Symposia, NF-kB and MAP kinase signaling in inflammation, 2016.
- Shin I, Won JH, Ko KA, Fujiwara E, Shin JH, Thomas T, Taunton J, Hosokawa H, Mochizuki N, Yeh ET, Abe J, Fujiwara K, and Le NT. The role of MAGI1 Ser741 phosphorylation in disturbed-flow induced endothelial dysfunction and atherosclerotic plaque formation. American Society for Cell Biology (ASCB) Annual Meeting I, 2015.
- Heo KS, Le NT, Yeh ET, Fujiwara K, Abe J. Disturbed flow-induced endothelial dysfunction is mediated by phosphorylation of Sentrin/SUMO-specific Protease 2 via activating p90RSK. Gordon Research Conference, 2015.
- Heo KS, Le NT, Yeh ET, Fujiwara K, Abe J. Disturbed flow-induced endothelial dysfunction is mediated by phosphorylation of Sentrin/SUMO-specific Protease 2 via activating p90RSK. Gordon Research Conference, 2015.
Book Chapters
- Abe J, Sandhu UG, Hoang NM, Thangam M, Quintana-Quezada RA, Fujiwara K, Le NT. Coordination of cellular localization-dependent effects of SUMOylation in regulating cardiovascular and neurological diseases. In: SUMO Regulation of Cellular Processes. Second edition. Springer, 2017.
Grant & Contract Support
| Title: | Mechanism of PECAM-1 tyrosine phosphorylation |
| Funding Source: | American Heart Association |
| Role: | Principal Investigator |
| Title: | Fluid shear stress signal transduction in endothelium |
| Funding Source: | NIH/NHLBI |
| Role: | Co-Principal Investigator |
| Title: | P90RSK: A flow responsive mediator of inflammation |
| Funding Source: | NIH/NHLBI |
| Role: | Co-Principal Investigator |
| Title: | Single molecule force spectroscopy analysis of PECAM-1 mechanotransduction |
| Funding Source: | NIH/NHLBI |
| Role: | Co-Principal Investigator |
| Title: | Cell adhesion molecule in endothelial mechanosignaling |
| Funding Source: | NIH/NHLBI |
| Role: | Principal Investigator |
| Title: | Role of PECAM-1 in stretch-activated signal transduction |
| Funding Source: | American Heart Association |
| Role: | Principal Investigator |
| Title: | Photonic-based implantable biosensors for drug discovery |
| Funding Source: | NYSTAR/CEIS in Bio-Imaging |
| Role: | Principal Investigator |
| Title: | Implantable cell-embedded biosensor for heart failure |
| Funding Source: | Clinical Translational Science Institute (CTSI) Grant |
| Role: | Co-Principal Investigator |
| Title: | PECAM-1 signaling in the flow-induced polarized endothelia response |
| Funding Source: | American Heart Association |
| Role: | Principal Investigator |
| Title: | Endothelial Vasculopathy and Telomere Dysfunction |
| Funding Source: | NIH/NHLBI |
| Role: | Co-Principal Investigator |
| Title: | Vascular Inflammation and atherosclerosis |
| Funding Source: | NIH/NHLBI |
| Role: | Director, Imaging Core |
| Title: | Effects of simulated microgravity and space radiation on human cells |
| Funding Source: | NASA |
| Role: | Co-Principal Investigator |
| Title: | Biomechanical Determinatants of Hematopoietic Stem Cell Potenital |
| Funding Source: | NIH/NHLBI |
| Role: | Co-Investigator |
| Title: | Disturbed flow-induced TERF2IP post-translational modifications and atherosclerosis |
| Funding Source: | NIH/NHLBI |
| Role: | Co-Investigator |
| Title: | p90RSK-ERK5 module, efferocytosis, and vulnerable plaque formation |
| Funding Source: | NIH/NHLBI |
| Role: | Co-Investigator |
| Title: | A novel role of MAGI-1 in regulating non-canonical LATS signaling and atherosclerotic plaque formation |
| Funding Source: | NIH/NHLBI |
| Role: | Co-Investigator |
| Title: | cART accelerates vascular aging in HIV infected patients |
| Funding Source: | NIH/NHLBI |
| Role: | Co-Investigator |