Marian Kalocsay
Department of Experimental Radiation Oncology, Division of Radiation Oncology
Present Title & Affiliation
Primary Appointment
Assistant Professor, Department of Experimental Radiation Oncology, Division of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX
Research Interests
We leverage proximity-proteomics and highly advanced mass spectrometry (MS) to study dynamics and aberrations of signaling processes in the context of immune checkpoints, pushing the envelope of existing MS technology. Through our innovative approaches we hope to elucidate ligand-specific differential immune signaling and decode differential phosphorylation in checkpoint signaling with the goal to ultimately better understand how the tumor micro-environment modulates receptor signaling.
Education & Training
Degree-Granting Education
| 2009 | LMU University of Munich and Max-Planck Institute of Biochemistry, Munich, DEU, PHD, Cell Biology |
| 2001 | University of Hannover, Hannover, DEU, Dipl, Biochemistry |
Postgraduate Training
| 2015-2017 | Research Fellowship, Mass Spectrometry, Harvard Medical School, Boston, MA |
| 2012-2015 | Research Fellowship, Chromatin Biology and Mass Spectrometry, Harvard Medical School, Boston, MA |
| 2011-2012 | Research Fellowship, European Molecular Biology Organization (EMBO), Harvard Medical School, Boston, MA |
| 2010-2010 | Research Fellowship, Molecular Biology, Max-Planck Institute of Biochemistry, Munich |
Experience & Service
Academic Appointments
Instructor, Department of Systems Biology, Harvard Medical School, Boston, MA, 2017 - 2022
Other Appointments/Responsibilities
Director Proteomics, Harvard Medical School, Boston, MA, 2017 - 2022
Associated Senior Research Scientist, Harvard Medical School, Boston, MA, 2016 - 2022
Honors & Awards
| 2020 | 2020 HUPO Early Career Researcher Competition, HUPO |
Selected Publications
Peer-Reviewed Articles
- Martin AP, Bradshaw GA, Eisert RJ, Egan ED, Tveriakhina L, Rogers JM, Dates AN, Scanavachi G, Aster JC, Kirchhausen T, Kalocsay M, Blacklow SC. A spatiotemporal Notch interaction map from plasma membrane to nucleus. Sci Signal 16(796):eadg6474, 2023. e-Pub 2023. PMID: 37527352.
- Susa KJ, Bradshaw GA, Eisert RJ, Schilling CM, Kalocsay M, Blacklow SC, Kruse AC. A Spatiotemporal Map of Co-Receptor Signaling Networks Underlying B Cell Activation. bioRxiv, 2023. e-Pub 2023. PMID: 36993395.
- Papa A, Zakharov SI, Katchman AN, Kushner JS, Chen BX, Yang L, Liu G, Jimenez AS, Eisert RJ, Bradshaw GA, Dun W, Ali SR, Rodriques A, Zhou K, Topkara V, Yang M, Morrow JP, Tsai EJ, Karlin A, Wan E, Kalocsay M, Pitt GS, Colecraft HM, Ben-Johny M, Marx SO. Rad regulation of CaV1.2 channels controls cardiac fight-or-flight response. Nat Cardiovasc Res 1(11):1022-1038, 2022. e-Pub 2022. PMID: 36424916.
- Fraser CS, Spetz JKE, Qin X, Presser A, Choiniere J, Li C, Yu S, Blevins F, Hata AN, Miller JW, Bradshaw GA, Kalocsay M, Sanchorawala V, Sarosiek S, Sarosiek KA. Exploiting endogenous and therapy-induced apoptotic vulnerabilities in immunoglobulin light chain amyloidosis with BH3 mimetics. Nat Commun 13(1):5789, 2022. e-Pub 2022. PMID: 36184661.
- Zhou H, Stein CB, Shafiq TA, Shipkovenska G, Kalocsay M, Paulo JA, Zhang J, Luo Z, Gygi SP, Adelman K, Moazed D. Rixosomal RNA degradation contributes to silencing of Polycomb target genes. Nature 604(7904):167-174, 2022. e-Pub 2022. PMID: 35355014.
- Jiménez A, Lu D, Kalocsay M, Berberich MJ, Balbi P, Jambhekar A, Lahav G. Time-series transcriptomics and proteomics reveal alternative modes to decode p53 oscillations. Mol Syst Biol 18(3):e10588, 2022. PMID: 35285572.
- Kushner JS, Liu G, Eisert RJ, Bradshaw GA, Pitt GS, Hinson JT, Kalocsay M, Marx SO. Detecting Cardiovascular Protein-Protein Interactions by Proximity Proteomics. Circ Res 130(2):273-287, 2022. e-Pub 2022. PMID: 35050691.
- Jiang B, Gao Y, Che J, Lu W, Kaltheuner IH, Dries R, Kalocsay M, Berberich MJ, Jiang J, You I, Kwiatkowski N, Riching KM, Daniels DL, Sorger PK, Geyer M, Zhang T, Gray NS. Discovery and resistance mechanism of a selective CDK12 degrader. Nat Chem Biol 17(6):675-683, 2021. e-Pub 2021. PMID: 33753926.
- Li L, Galichon P, Xiao X, Figueroa-Ramirez AC, Tamayo D, Lee JJ, Kalocsay M, Gonzalez-Sanchez D, Chancay MS, McCracken KW, Lee NN, Ichimura T, Mori Y, Valerius MT, Wilflingseder J, Lemos DR, Edelman ER, Bonventre JV. Orphan nuclear receptor COUP-TFII enhances myofibroblast glycolysis leading to kidney fibrosis. EMBO Rep 22(6):e51169, 2021. e-Pub 2021. PMID: 34031962.
- May EA, Kalocsay M, D'Auriac IG, Schuster PS, Gygi SP, Nachury MV, Mick DU. Time-resolved proteomics profiling of the ciliary Hedgehog response. J Cell Biol 220(5), 2021. PMID: 33856408.
- Drouin A, Wallbillich N, Theberge M, Liu S, Katz J, Bellovoda K, Se Yun Cheon S, Gootkind F, Bierman E, Zavras J, Berberich MJ, Kalocsay M, Guastaldi F, Salvadori N, Troulis M, Fusco DN. Impact of Zika virus on the human type I interferon osteoimmune response. Cytokine 137:155342, 2021. e-Pub 2020. PMID: 33130337.
- Mehta AK, Cheney EM, Hartl CA, Pantelidou C, Oliwa M, Castrillon JA, Lin JR, Hurst KE, de Oliveira Taveira M, Johnson NT, Oldham WM, Kalocsay M, Berberich MJ, Boswell SA, Kothari A, Johnson S, Dillon DA, Lipschitz M, Rodig S, Santagata S, Garber JE, Tung N, Yélamos J, Thaxton JE, Mittendorf EA, Sorger PK, Shapiro GI, Guerriero JL. Targeting immunosuppressive macrophages overcomes PARP inhibitor resistance in BRCA1-associated triple-negative breast cancer. Nat Cancer 2(1):66-82, 2021. e-Pub 2020. PMID: 33738458.
- Shipkovenska G, Durango A, Kalocsay M, Gygi SP, Moazed D. A conserved RNA degradation complex required for spreading and epigenetic inheritance of heterochromatin. Elife 9, 2020. e-Pub 2020. PMID: 32491985.
- Santoriello C, Sporrij A, Yang S, Flynn RA, Henriques T, Dorjsuren B, Custo Greig E, McCall W, Stanhope ME, Fazio M, Superdock M, Lichtig A, Adatto I, Abraham BJ, Kalocsay M, Jurynec M, Zhou Y, Adelman K, Calo E, Zon LI. RNA helicase DDX21 mediates nucleotide stress responses in neural crest and melanoma cells. Nat Cell Biol 22(4):372-379, 2020. e-Pub 2020. PMID: 32231306.
- Pan J, Qian X, Lattmann S, El Sahili A, Yeo TH, Jia H, Cressey T, Ludeke B, Noton S, Kalocsay M, Fearns R, Lescar J. Structure of the human metapneumovirus polymerase phosphoprotein complex. Nature 577(7789):275-279, 2020. e-Pub 2019. PMID: 31698413.
- Liu G, Papa A, Katchman AN, Zakharov SI, Roybal D, Hennessey JA, Kushner J, Yang L, Chen BX, Kushnir A, Dangas K, Gygi SP, Pitt GS, Colecraft HM, Ben-Johny M, Kalocsay M, Marx SO. Mechanism of adrenergic CaV1.2 stimulation revealed by proximity proteomics. Nature 577(7792):695-700, 2020. e-Pub 2020. PMID: 31969708.
- Mugahid D, Kalocsay M, Liu X, Gruver JS, Peshkin L, Kirschner MW. YAP regulates cell size and growth dynamics via non-cell autonomous mediators. Elife 9, 2020. e-Pub 2020. PMID: 31913124.
- Nusinow DP, Szpyt J, Ghandi M, Rose CM, McDonald ER, Kalocsay M, Jané-Valbuena J, Gelfand E, Schweppe DK, Jedrychowski M, Golji J, Porter DA, Rejtar T, Wang YK, Kryukov GV, Stegmeier F, Erickson BK, Garraway LA, Sellers WR, Gygi SP. Quantitative Proteomics of the Cancer Cell Line Encyclopedia. Cell 180(2):387-402.e16, 2020. PMID: 31978347.
- Wang Y, Dix MM, Bianco G, Remsberg JR, Lee HY, Kalocsay M, Gygi SP, Forli S, Vite G, Lawrence RM, Parker CG, Cravatt BF. Expedited mapping of the ligandable proteome using fully functionalized enantiomeric probe pairs. Nat Chem 11(12):1113-1123, 2019. e-Pub 2019. PMID: 31659311.
- Ferguson FM, Doctor ZM, Ficarro SB, Browne CM, Marto JA, Johnson JL, Yaron TM, Cantley LC, Kim ND, Sim T, Berberich MJ, Kalocsay M, Sorger PK, Gray NS. Discovery of Covalent CDK14 Inhibitors with Pan-TAIRE Family Specificity. Cell Chem Biol 26(6):804-817.e12, 2019. e-Pub 2019. PMID: 30930164.
- Kalocsay M. APEX Peroxidase-Catalyzed Proximity Labeling and Multiplexed Quantitative Proteomics. Methods Mol Biol 2008:41-55, 2019. PMID: 31124087.
- Iglesias N, Currie MA, Jih G, Paulo JA, Siuti N, Kalocsay M, Gygi SP, Moazed D. Automethylation-induced conformational switch in Clr4 (Suv39h) maintains epigenetic stability. Nature 560(7719):504-508, 2018. e-Pub 2018. PMID: 30051891.
- Brumbaugh J, Di Stefano B, Wang X, Borkent M, Forouzmand E, Clowers KJ, Ji F, Schwarz BA, Kalocsay M, Elledge SJ, Chen Y, Sadreyev RI, Gygi SP, Hu G, Shi Y, Hochedlinger K. Nudt21 Controls Cell Fate by Connecting Alternative Polyadenylation to Chromatin Signaling. Cell 172(1-2):106-120.e21, 2018. e-Pub 2017. PMID: 29249356.
- Brumbaugh J, Di Stefano B, Wang X, Borkent M, Forouzmand E, Clowers KJ, Ji F, Schwarz BA, Kalocsay M, Elledge SJ, Chen Y, Sadreyev RI, Gygi SP, Hu G, Shi Y, Hochedlinger K. Nudt21 Controls Cell Fate by Connecting Alternative Polyadenylation to Chromatin Signaling. Cell 172(3):629-631, 2018. PMID: 29373832.
- Paek J, Kalocsay M, Staus DP, Wingler L, Pascolutti R, Paulo JA, Gygi SP, Kruse AC. Multidimensional Tracking of GPCR Signaling via Peroxidase-Catalyzed Proximity Labeling. Cell 169(2):338-349.e11, 2017. PMID: 28388415.
- Renkawitz J, Lademann CA, Kalocsay M, Jentsch S. Monitoring homology search during DNA double-strand break repair in vivo. Mol Cell 50(2):261-72, 2013. e-Pub 2013. PMID: 23523370.
- Kalocsay M, Hiller NJ, Jentsch S. Chromosome-wide Rad51 spreading and SUMO-H2A.Z-dependent chromosome fixation in response to a persistent DNA double-strand break. Mol Cell 33(3):335-43, 2009. PMID: 19217407.
- Jasiak AJ, Hartmann H, Karakasili E, Kalocsay M, Flatley A, Kremmer E, Strässer K, Martin DE, Söding J, Cramer P. Genome-associated RNA polymerase II includes the dissociable Rpb4/7 subcomplex. J Biol Chem 283(39):26423-7, 2008. e-Pub 2008. PMID: 18667430.
- Rape M, Hoppe T, Gorr I, Kalocay M, Richly H, Jentsch S. Mobilization of processed, membrane-tethered SPT23 transcription factor by CDC48(UFD1/NPL4), a ubiquitin-selective chaperone. Cell 107(5):667-77, 2001. PMID: 11733065.
Grant & Contract Support
| Title: | |
| Funding Source: | Cancer Prevention & Research Institute of Texas (CPRIT) |
| Role: | Principal Investigator |
| Title: | |
| Funding Source: | UT STARS |
| Role: | Principal Investigator |
Patient Reviews
CV information above last modified September 26, 2024