About Dr. Wang
Dr. Wang is an Associate Professor in the Department of Genetics. She obtained her Ph.D. with Dr. Adam Kuspa at the Baylor College of Medicine and conducted her postdoctoral fellowship with Dr. Stephen J. Elledge at the Harvard Medical School and Brigham and Women’s Hospital. Her laboratory research is focused on understanding how cells respond to DNA damage and safeguard the integrity of the genome. Examples of their current research projects are: (1) to understand how the hereditary breast tumor suppressor BRCA1 interaction network suppresses breast tumor development; (2) to understand how chromatin modification at sites of DNA damage regulates DNA repair and transcription; (3) to investigate the mechanism for protection of genome stability in response to DNA replication stress for prevention of cancer. They have established a mouse model that interrogates the role of Abraxas-mediated BRCA1 signaling in breast tumor suppression. Their recent work also revealed an ionizing radiation (IR)-induced, Abraxas-dependent BRCA1 dimerization mechanism in the BRCA1 tumor suppression pathway, allowing assessment of the functional importance of several previously uncharacterized BRCA1 germline mutations. A recent study from Dr. Wang’s laboratory has uncovered an RNF8- and Ube2S-catalyzed Lys11-linkage-specific ubiquitin modification on damaged chromatin that regulates repression of transcription at DNA double strand breaks, revealing Lys11-linkage ubiquitin modification as a new regulatory platform in the DNA damage response. Recently, they also have identified and characterized a new component in the response to replication stress that protects nascent DNA integrity at stalled replication forks independently of BRCA1/BRCA2 for maintenance of genome stability. The overall goal of her research is to decipher the complex DNA response network that protects genome integrity and prevents the development of cancer.
View a compelte list of Dr. Wang's publications.
Associate Professor, Department of Genetics, Division of Basic Science Research, The University of Texas MD Anderson Cancer Center, Houston, TX
Associate Professor, UTMD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, Houston, TX
DNA damage response Genomic instability and Cancer BRCA1 signaling Ubiquitin signaling
Defects in the ability of cells to properly respond to and repair DNA damage result in genomic instability and underlie many forms of cancer. The goal of our research is to understand how cells respond to DNA damage and safeguard the integrity of the genome.
Cells have evolved a complex sensory network called the DNA damage response (DDR) to respond to genotoxic stress. The DDR coordinates cell cycle regulation, DNA repair, chromatin reorganization and transcription in response to damage. Once DNA damage is sensed, a phosphorylation signaling cascade is initiated by the central DNA damage kinases, ATM and ATR. Recently, a DNA damage induced ubiquitin signaling pathway has also emerged that works together with phosphorylation-dependent signaling to recruit essential repair factors to the sites of DNA damage. Damage-induced ATM/ATR phosphorylation on S139 of histone H2AX directly recruits MDC1 through MDC1’s BRCT domains. MDC1 itself is a substrate of ATM/ATR, and its phosphorylation leads to the recruitment of a ubiquitin E2/E3 ligase RNF8/UBC13 to damage sites. We are seeking to identify additional players in this signaling cascade and investigate the role of each player in the DNA damage repair and signaling, as well as its role in cancer progression.
The RNF8/Ubc13-dependent ubiquitination on the damaged chromatin creates docking sites for BRCA1 complexes. The BRCA1 tumor suppressor is associated with hereditary breast and ovarian cancer and plays critical roles in DNA repair, cell cycle checkpoint control and maintenance of genomic stability. BRCA1 contains two C-terminal BRCT repeats and a N-terminal RING domain that binds BARD1 forming an E3 ubiqutin ligase. Recently we have identified a novel BRCA1 associated protein complex, the Rap80/Abraxas/BRCA1 complex (BRCA1-A complex). In this complex, Abraxas contains a phosphorylated SPxF motif at its C-terminus that associates with the BRCT domain of BRCA1. The BRCA1-A complex also contains a deubiquitination enzyme BRCC36. We are currently investigating how the BRCA1-A complex is involved in BRAC1’s function in breast tumor suppression.
In summary, we are interested in using various biochemical and genetic approaches, such as gene knockouts, mass spectrometry and genetic screens using siRNA libraries, to identify important players in the DNA damage response and define the roles of these proteins in maintaining genomic stability and tumor suppression.
View a complete list of Dr. Wang's publications.
|2000||Baylor College of Medicine, Houston, TX, USA, PHD, Biochemistry and Molecular Biology|
|1994||Shanghai Institute of Biochemistry, Shanghai, CHN, MS, Molecular Biology|
|1991||Nanjing University, Nanjing, CHN, BS, Biochemistry|
|2003-2008||Postdoctoral Fellow, Brigham and Women's Hospital/Harvard Medical School, Boston, MA|
|2000-2003||Postdoctoral Fellow, Baylor College of Medicine, Houston, TX|
Assistant Professor, Department of Genetics, Division of Basic Science Research, The University of Texas MD Anderson Cancer Center, Houston, TX, 2008 - 2014
Assistant Professor, UTMD Anderson Cancer Center UT Health Graduate School of Biomedical Sciences, Houston, TX, 2008 - 2014
- Xu S, Wu X, Wu L, Castillo A, Liu J, Atkinson E, Paul A, Su D, Schlacher K, Komatsu Y, You MJ, Wang B. Abro1 maintains genome stability and limits replication stress by protecting replication fork stability. Genes Dev 31(14):1469-1482, 2017. PMID: 28860160.
- Paul A, Wang B. RNF8- and Ube2S-Dependent Ubiquitin Lysine 11-Linkage Modification in Response to DNA Damage. Mol Cell 66(4):458-472, 2017. PMID: 28525740.
- Wu Q, Paul A, Su D, Mehmood S, Foo TK, Ochi T, Bunting EL, Xia B, Robinson CV, Wang B*, Blunder TL*. Structure of BRCA1-BRCT/Abraxas Complex Reveals Phosphorylation-Dependent BRCT Dimerization at DNA Damage Sites. *Co-corresponding authors. Mol Cell 61(3):434-48, 2016. e-Pub 2016. PMID: 26778126.
- Castillo A, Paul A, Sun B, Huang TH, Wang Y, Yazinski SA, Tyler J, Li L, You MJ, Zou L, Yao J, Wang B. The BRCA1-Interacting Protein Abraxas Is Required for Genomic Stability and Tumor Suppression. Cell Rep 8(3):807-17, 2014. e-Pub 2014. PMID: 25066119.
- Hu X, Paul A, Wang B. Rap80 protein recruitment to DNA double-strand breaks requires binding to both small ubiquitin-like modifier (SUMO) and ubiquitin conjugates. J Biol Chem 287(30):25510-9, 2012. e-Pub 2012. PMID: 22689573.
- Hu X, Kim JA, Castillo A, Huang M, Liu J, Wang B. NBA1/Merit40 and BRE interaction is required for the integrity of two distinct deubiquitinating enzyme BRCC36 containing complexes. J Biol Chem 286(13):11734-45, 2011. e-Pub 2011. PMID: 21282113.
- Wang B, Hurov K, Hofmann K, Elledge SJ. NBA1, a new player in the Brca1 A complex, is required for DNA damage resistance and checkpoint control. Genes Dev 23(6):729-39, 2009. PMID: 19261749.
- Xiao A, Li H, Schecter D, Ahn SH, Fabrizio LA, Erdjument-Bromage H, Ishibe-Murakami S, Wang B, Tempst P, Hofmann K, Patel DJ, Elledge SJ, Allis CD. WSTF regulates the H2A.X DNA damage response via a novel tyrosine kinase activity. Nature 457(7225):57-62, 2009. e-Pub 2008. PMID: 19092802.
- Wang B, Elledge SJ. Ubc 13/Rnf8 ubiquitin ligase controls foci formation of the Rap80/Abraxas/Brca1/Brcc36 complex in response to DNA damage. Proc Natl Acad Sci U S A 104(52):20759-63, 2007. e-Pub 2007. PMID: 18077395.
- Wang B, Matsuoka S, Ballif BA, Zhang D, Smogorzewska A, Gygi S, Elledge SJ. Abraxas and Rap80 form a BRCA1 protein complex required for the DNA damage response. Science 316(5828):1194-8, 2007. PMID: 17525340.
- Stewart G, Wang B, Bignell CR, Taylor AM, Elledge SJ. MDC1 is a mediator of the mammalian DNA damage checkpoint. Nature 421(6926):961-6, 2003. PMID: 12607005.
- Wang B, Matsuoka S, Carpenter PB, Elledge SJ. 53BP1 a mediator of the DNA damage checkpoint. Science 298(5597):1435-8, 2002. PMID: 12364621.
|Title:||The role of a BRCA1-associated complex in DNA damage response and tumor suppression|
|Title:||Characterizing Abrol in protection of stalled replication fork integrity and tumor suppression|
|Funding Source:||MDACC IRG|
|Title:||Functional analysis of linage-specific ubiquitin modification at double strand breaks|
|Funding Source:||Cancer Prevention & Research Institute of Texas (CPRIT)|