Blaine Bartholomew, Ph.D.
Department of Epigenetics and Molecular Carcinogenesis, Division of Division of Discovery Science
Present Title & Affiliation
Primary Appointment
Deputy Department Chair, Department of Epigenetics and Molecular Carcinogenesis, Division of Division of Discovery Science, The University of Texas MD Anderson Cancer Center, Houston, TX
Professor, Department of Epigenetics and Molecular Carcinogenesis, Division of Division of Discovery Science, The University of Texas MD Anderson Cancer Center, Houston, TX
Dual/Joint/Adjunct Appointment
Professor, Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, Houston, TX
Research Interests
My research interest is on the structure and function of ATP-dependent chromatin remodeling complexes and their mechanisms of nucleosome mobilization and reorganization. Given the size and complexity of these complexes, it has been essential for my lab to work on approaches for studying the physical interactions of remodelers with their nucleosomal substrates. I have taken a chemical biology type approach and have applied targeted protein and DNA modifications to map specific protein-protein and protein-DNA contacts. The reporters tethered to specific target sites have been of the photochemical, proteolytic, and fluorescent types. My lab uses Saccharomyces cerevisiae as our model system because once a particular contact has been found and mapped, we have been able to mutate that region in the protein and find the in vivo effects of these mutations on chromatin structure and function as well as the effects on its biochemical activity in vitro. I am applying related approaches to examine changes in nucleosome structure when remodeled and have developed an approach for monitoring the interactions of several locations in the histone octamer with nucleosomal DNA. My lab is expanding and improving this technique to obtain more information about temporal changes in nucleosome structure. I am also interested in using single molecule approaches to examine nucleosome remodeling and have been involved in two collaborations to use single molecule Fluorescence Resonance Energy Transfer and DNA methylation accessibility with bisulfite sequencing to examine SWI/SNF and ISW2 remodeling. I am interested in finding the roles of histone modifications such as acetylation and methylation in the recruitment and regulation of the chromatin remodeler. I am also involved in studying the interplay of histone chaperones with ATP-dependent chromatin remodelers
Education & Training
Degree-Granting Education
| 1988 | University of California, Davis, Davis, California, US, Chemistry, Ph.D |
| 1982 | Brigham Young University, Provo, Utah, US, Chemistry, BS |
Postgraduate Training
| 1988-1991 | Postdoctoral, Gene Regulation, University of California, San Diego, San Diego, California |
Experience & Service
Academic Appointments
Professor, Department of School of Medicine, Southern Illinois University, Cardondale, IL, 2004 - 2013
Associate Professor, Department of School of Medicine, Southern Illinois University, Cardondale, IL, 1997 - 2004
Assistant Professor, Department of School of Medicine, Southern Illinois University, Cardondale, IL, 1991 - 1997
Administrative Appointments/Responsibilities
Deputy Department Chair, Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, Houston, TX, 2023 - Present
Committee Member, NIH-NICHD-Eunice Kennedy Shriver - Site Visit, Smithville, TX, 2017 - 2017
Committee Member, Department of MGB Study Section, National Institutes of Health, Smithville, TX, 2016 - 2022
Committee Member, Department of Nucleomics Tools, ZRG1-CB-D, National Human Genome Research Institute, Smithville, TX, 2015 - 2015
Committee Member, National Human Genome Research Institute Special Emphasis Panel, Smithville, TX, 2014 - 2014
Committee Member, National Human Genome Research Institute Special Emphasis Panel (ZHG1 HGR-M), Carbondale, IL, 2011 - 2011
Ad Hoc Member, NIH-GMS MGA Study Section, Carbondale, IL, 2010 - 2010
Ad Hoc Member, NIH-GMS MGC Study Section, Carbondale, IL, 2010 - 2010
Committee Member, NIH Genes, Genomes, and Genetics Fellowship Study Section, Carbondale, IL, 2008 - 2008
Ad Hoc Member, NIH-GMS MGB Study section, Carbondale, IL, 2008 - 2009
Committee Member, NIH Institute of Aging Project Study Section, Carbondale, IL, 2007 - 2007
Committee Chair, American Cancer Society Genetic Mechanisms in Cancer, Carbondale, IL, 2006 - 2006
Committee Vice Chair, American Cancer Society (National) Scientific Review for Genetic Mechanisms in Cancer, Carbondale, IL, 2005 - 2005
Committee Member, NIH Program Project Study Section-Child Development Special Emphasis Panel, Carbondale, IL, 2003 - 2003
Committee Member, American Cancer Society (National) Scientific Review for Genetic Mechanisms in Cancer, Carbondale, IL, 2002 - 2006
Ad Hoc Member, NIH Study Section CDF-1, Carbondale, IL, 2002 - 2002
Other Appointments/Responsibilities
Member, SIU Faculty Senate, Carbondale, IL, 2011 - 2013
Committee Member, SIU Research Scholar Review Committee, Carbondale, IL, 2011 - 2012
Committee Member, SIU Departmental Search Committee, Carbondale, IL, 2008 - 2008
Committee Member, SIU Office of Research and Develop. Special Grants Review Committee, Carbondale, IL, 2007 - 2007
Committee Member, SIU School of Medicine Tenure and Promotions Committee, Carbondale, IL, 2007 - 2010
Committee Member, SIU Departmental Search Committee, Carbondale, IL, 2006 - 2006
Organizer, SIU MBMB Seminar, Carbondale, IL, 2004 - 2005
Committee Member, SIU Departmental Search Committee, Carbondale, IL, 2003 - 2003
Chair, SIU MBMB Program Operating Paper Committee, Carbondale, IL, 2002 - 2002
Committee Member, SIU Development of integrated curriculum for MBMB graduate program, Carbondale, IL, 2002 - 2002
Committee Member, SIU Reorganization of Biochemistry Curriculum in Sequence 1, Carbondale, IL, 1999 - 1999
Organizer, SIU MBMB Seminar, Carbondale, IL, 1999 - 2000
Committee Member, SIU Graduate Student Admission Committee, Carbondale, IL, 1997 - 2013
Institutional Committee Activities
Committee Member, GSBS Membership Committee, 2023 - Present
Committee Member, Institutional Compliance Committee, 2023 - Present
Committee Member, Curriculum Committee, 2021 - Present
Co-organizer, Epigenetics and Molecular Carcinogenesis Department Seminar Series, 2021 - Present
Committee Member, Faculty Academic Review Committee, 2019 - Present
Committee Member, GEC, 2016 - 2016
Co-Director, GSBS, 2015 - Present
Member, GSBS, 2014 - 2015
Committee Member, Department of Epigenetics and Molecular Carcinogenesis Promotion and Tenure Committee, 2014 - Present
Committee Member, Graduate Program, 2014 - 2014
Committee Member, Steering Committee of the GSBS Program at Science Park, 2014 - Present
Co-Chair, SIU Molecular and Cellular Biology Symposium, 2004 - 2006
Committee Member, SIU Central Research Committee, 2001 - 2004
Subcommittee Member, SIU Research Pathways Subcommittee for Curriculum, 2000 - 2000
Chair, SIU Molecular and Cellular Biology Symposium, 1994 - 2003
Honors & Awards
| 2006 | Excellence through Commitment Award, Outstanding Scholar, Southern Illinois University |
| 2002 | American Cancer Society Researcher Award |
| 2001 | American Cancer Society Researcher Award |
| 2000 | American Cancer Society Researcher Award |
| 1999 | American Cancer Society Researcher Award |
| 1991 | American Cancer Society Fellow, University of California, San Diego, Center for Molecular Genetics |
| 1990 | American Cancer Society Fellow, University of California, San Diego, Center for Molecular Genetics |
| 1989 | American Cancer Society Fellow, University of California, San Diego, Center for Molecular Genetics |
| 1988 | American Cancer Society Fellow, University of California, San Diego, Center for Molecular Genetics |
Professional Memberships
Selected Publications
Peer-Reviewed Articles
- Saha, D, Animireddy, S, Lee, J, Thommen, A, Murvin, MM, Lu, Y, Calabrese, JM, Bartholomew, B. Enhancer switching in cell lineage priming is linked to eRNA, Brg1’s AT-hook, and SWI/SNF recruitment. Molecular cell 84(10):1855-1869.e5, 2024. PMID: 38593804.
- Saha D, Hailu S, Hada A, Lee J, Luo J, Ranish JA, Lin YC, Feola K, Persinger J, Jain A, Liu B, Lu Y, Sen P, Bartholomew B. The AT-hook is an evolutionarily conserved auto-regulatory domain of SWI/SNF required for cell lineage priming. Nat Commun 14(1):4682, 2023. e-Pub 2023. PMID: 37542049.
- Bhardwaj SK, Hailu SG, Olufemi L, Brahma S, Kundu S, Hota SK, Persinger J, Bartholomew B. Dinucleosome specificity and allosteric switch of the ISW1a ATP-dependent chromatin remodeler in transcription regulation. Nat Commun 11(1):5913, 2020. e-Pub 2020. PMID: 33219211.
- He W, Zhang L, Villarreal OD, Fu R, Bedford E, Dou J, Patel AY, Bedford MT, Shi X, Chen T, Bartholomew B, Xu H. Author Correction: De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens. Nat Commun 11(1):1134, 2020. e-Pub 2020. PMID: 32098955.
- He W, Zhang L, Villarreal OD, Fu R, Bedford E, Dou J, Patel AY, Bedford MT, Shi X, Chen T, Bartholomew B, Xu H. De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens. Nat Commun 10(1):4541, 2019. e-Pub 2019. PMID: 31586052.
- Hada A, Hota SK, Luo J, Lin YC, Kale S, Shaytan AK, Bhardwaj SK, Persinger J, Ranish J, Panchenko AR, Bartholomew B. Histone Octamer Structure Is Altered Early in ISW2 AT-Dependent Nucleosome Remodeling. Cell Rep 28(1):282-294.e6, 2019. PMID: 31269447.
- Brahma S, Ngubo M, Paul S, Udugama M, Bartholomew B. The Arp8 and Arp4 module acts as a DNA sensor controlling INO80 chromatin remodeling. Nat Commun 9(1):3309, 2018. e-Pub 2018. PMID: 30120252.
- Brahma S, Udugama MI, Kim J, Hada A, Bhardwaj SK, Hailu SG, Lee TH, Bartholomew B. INO80 exchanges H2A.Z for H2A by translocating on DNA proximal to histone dimers. Nat Commun 8:15616, 2017. e-Pub 2017. PMID: 28604691.
- Sen P, Luo J, Hada A, Hailu SG, Dechassa ML, Persinger J, Brahma S, Paul S, Ranish J, Bartholomew B. Loss of Snf5 Induces Formation of an Aberrant SWI/SNF Complex. Cell Rep 18(9):2135-2147, 2017. PMID: 28249160.
- Prasad R, D'Arcy S, Hada A, Luger K, Bartholomew B. Coordinated action of Nap1 and RSC in disassembly of tandem nucleosomes. Mol Cell Biol 36(17):2262-71, 2016. e-Pub 2016. PMID: 27273866.
- Harada BT, Hwang WL, Deindl S, Chatterjee N, Bartholomew B, Zhuang X. Stepwise nucleosome translocation by RSC remodeling complexes. Elife 5, 2016. e-Pub 2016. PMID: 26895087.
- Lafon A, Taranum S, Pietrocola F, Dingli F, Loew D, Brahma S, Bartholomew B, Papamichos-Chronakis M. INO80 Chromatin Remodeler Facilitates Release of RNA Polymerase II from Chromatin for Ubiquitin-Mediated Proteasomal Degradation. Mol Cell 60(5):784-796, 2015. e-Pub 2015. PMID: 26656161.
- Chatterjee N, North JA, Dechassa ML, Manohar M, Prasad R, Luger K, Ottesen JJ, Poirier MG, Bartholomew B. Histone Acetylation near the Nucleosome Dyad Axis Enhances Nucleosome Disassembly by RSC and SWI/SNF. Mol Cell Biol 35(23):4083-92, 2015. e-Pub 2015. PMID: 26416878.
- Kim SA, Chatterjee N, Jennings MJ, Bartholomew B, Tan S. Extranucleosomal DNA enhances the activity of the LSD1/CoREST histone demethylase complex. Nucleic Acids Res 43(10):4868-80, 2015. e-Pub 2015. PMID: 25916846.
- Kapoor P, Bao Y, Xiao J, Luo J, Shen J, Persinger J, Peng G, Ranish J, Bartholomew B, Shen X. Regulation of Mec1 kinase activity by the SWI/SNF chromatin remodeling complex. Genes Dev 29(6):591-602, 2015. PMID: 25792597.
- Li M, Hada A, Sen P, Olufemi L, Hall MA, Smith BY, Forth S, McKnight JN, Patel A, Bowman GD, Bartholomew B, Wang MD. Dynamic regulation of transcription factors by nucleosome remodeling. Elife 4, 2015. e-Pub 2015. PMID: 26047462.
- Creamer KM, Job G, Shanker S, Neale GA, Lin YC, B B, Partridge JF. The Mi-2 Homolog Mit1 Actively Positions Nucleosomes within Heterochromatin To Suppress Transcription. Mol Cell Biol 34(11):2046-61, 2014. e-Pub 2014. PMID: 24662054.
- Bartholomew B. Monomeric actin required for INO80 remodeling. Nat Struct Mol Biol 20(4):405-7, 2013. PMID: 23552289.
- Hota SK, Bhardwaj SK, Deindl S, Lin YC, Zhuang X, Bartholomew B. Nucleosome mobilization by ISW2 requires the concerted action of the ATPase and SLIDE domains. Nat Struct Mol Biol 20(2):222-9, 2013. e-Pub 2013. PMID: 23334290.
- Deindl S, Hwang WL, Hota SK, Blosser TR, Prasad P, Bartholomew B, Zhuang X. ISWI remodelers slide nucleosomes with coordinated multi-base-pair entry steps and single-base-pair exit steps. Cell 152(3):442-52, 2013. PMID: 23374341.
- Sen P, Vivas P, Dechassa ML, Mooney AM, Poirier MG, Bartholomew B. The SnAC domain of SWI/SNF is a histone anchor required for remodeling. Mol Cell Biol 33(2):360-70, 2013. e-Pub 2012. PMID: 23149935.
- Dechassa ML, Hota SK, Sen P, Chatterjee N, Prasad P, Bartholomew B. Disparity in the DNA translocase domains of SWI/SNF and ISW2. Nucleic Acids Res 40(10):4412-21, 2012. e-Pub 2012. PMID: 22298509.
- Hota SK, Dechassa ML, Prasad P, Bartholomew B. Mapping protein-DNA and protein-protein interactions of ATP-dependent chromatin remodelers. Methods Mol Biol 809:381-409, 2012. PMID: 22113290.
- Hota SK, Bartholomew B. Approaches for studying nucleosome movement by ATP-dependent chromatin remodeling complexes. Methods Mol Biol 809:367-80, 2012. PMID: 22113289.
- Darst RP, Pardo CE, Pondugula S, Gangaraju VK, Nabilsi NH, Bartholomew B, Kladde MP. Simultaneous single-molecule detection of endogenous C-5 DNA methylation and chromatin accessibility using MAPit. Methods Mol Biol 833:125-41, 2012. PMID: 22183592.
- Sen P, Ghosh S, Pugh BF, Bartholomew B. A new, highly conserved domain in Swi2/Snf2 is required for SWI/SNF remodeling. Nucleic Acids Res 39(21):9155-66, 2011. e-Pub 2011. PMID: 21835776.
- Chatterjee N, Sinha D, Lemma-Dechassa M, Tan S, Shogren-Knaak MA, Bartholomew B. Histone H3 tail acetylation modulates ATP-dependent remodeling through multiple mechanisms. Nucleic Acids Res 39(19):8378-91, 2011. e-Pub 2011. PMID: 21749977.
- North JA, Javaid S, Ferdinand MB, Chatterjee N, Picking JW, Shoffner M, Nakkula RJ, Bartholomew B, Ottesen JJ, Fishel R, Poirier MG. Phosphorylation of histone H3(T118) alters nucleosome dynamics and remodeling. Nucleic Acids Res 39(15):6465-74, 2011. e-Pub 2011. PMID: 21576235.
- Gaykalova DA, Nagarajavel V, Bondarenko VA, Bartholomew B, Clark DJ, Studitsky VM. A polar barrier to transcription can be circumvented by remodeler-induced nucleosome translocation. Nucleic Acids Res 39(9):3520-8, 2011. e-Pub 2011. PMID: 21245049.
- Udugama M, Sabri A, Bartholomew B. The INO80 ATP-dependent chromatin remodeling complex is a nucleosome spacing factor. Mol Cell Biol 31(4):662-73, 2011. e-Pub 2010. PMID: 21135121.
- Dechassa ML, Sabri A, Pondugula S, Kassabov SR, Chatterjee N, Kladde MP, Bartholomew B. SWI/SNF has intrinsic nucleosome disassembly activity that is dependent on adjacent nucleosomes. Mol Cell 38(4):590-602, 2010. PMID: 20513433.
- Prasad P, Bartholomew B. Control of nucleosome movement: to space or not to space nucleosomes?. Epigenetics 5(4):282-6, 2010. e-Pub 2010. PMID: 20421732.
- Ransom M, Williams SK, Dechassa ML, Das C, Linger J, Adkins M, Liu C, Bartholomew B, Tyler JK. FACT and the proteasome promote promoter chromatin disassembly and transcriptional initiation. J Biol Chem 284(35):23461-71, 2009. e-Pub 2009. PMID: 19574230.
- Gangaraju VK, Prasad P, Srour A, Kagalwala MN, Bartholomew B. Conformational changes associated with template commitment in ATP-dependent chromatin remodeling by ISW2. Mol Cell 35(1):58-69, 2009. PMID: 19595716.
- Persinger J, Bartholomew B. Site-directed DNA crosslinking of large multisubunit protein-DNA complexes. Methods Mol Biol 543:453-74, 2009. PMID: 19378181.
- Dechassa ML, Zhang B, Horowitz-Scherer R, Persinger J, Woodcock CL, Peterson CL, Bartholomew B. Architecture of the SWI/SNF-nucleosome complex. Mol Cell Biol 28(19):6010-21, 2008. e-Pub 2008. PMID: 18644858.
- Dang W, Bartholomew B. Domain architecture of the catalytic subunit in the ISW2-nucleosome complex. Mol Cell Biol 27(23):8306-17, 2007. e-Pub 2007. PMID: 17908792.
- Dang W, Kagalwala MN, Bartholomew B. The Dpb4 subunit of ISW2 is anchored to extranucleosomal DNA. J Biol Chem 282(27):19418-25, 2007. e-Pub 2007. PMID: 17491017.
- Gangaraju VK, Bartholomew B. Dependency of ISW1a chromatin remodeling on extranucleosomal DNA. Mol Cell Biol 27(8):3217-25, 2007. e-Pub 2007. PMID: 17283061.
- Dang W, Kagalwala MN, Bartholomew B. Regulation of ISW2 by concerted action of histone H4 tail and extranucleosomal DNA. Mol Cell Biol 26(20):7388-96, 2006. PMID: 17015471.
- Zofall M, Persinger J, Kassabov SR, Bartholomew B. Chromatin remodeling by ISW2 and SWI/SNF requires DNA translocation inside the nucleosome. Nat Struct Mol Biol 13(4):339-46, 2006. e-Pub 2006. PMID: 16518397.
- Auty R, Steen H, Myers LC, Persinger J, Bartholomew B, Gygi SP, Buratowski S. Purification of active TFIID from Saccharomyces cerevisiae. Extensive promoter contacts and co-activator function. J Biol Chem 279(48):49973-81, 2004. e-Pub 2004. PMID: 15448131.
- Zofall M, Persinger J, Bartholomew B. Functional role of extranucleosomal DNA and the entry site of the nucleosome in chromatin remodeling by ISW2. Mol Cell Biol 24(22):10047-57, 2004. PMID: 15509805.
- Kagalwala MN, Glaus BJ, Dang W, Zofall M, Bartholomew B. Topography of the ISW2-nucleosome complex: insights into nucleosome spacing and chromatin remodeling. EMBO J 23(10):2092-104, 2004. e-Pub 2004. PMID: 15131696.
- Kassabov SR, Bartholomew B. Site-directed histone-DNA contact mapping for analysis of nucleosome dynamics. Methods Enzymol 375:193-210, 2004. PMID: 14870668.
- Kassabov SR, Zhang B, Persinger J, Bartholomew B. SWI/SNF unwraps, slides, and rewraps the nucleosome. Mol Cell 11(2):391-403, 2003. PMID: 12620227.
- Vary JC, Gangaraju VK, Qin J, Landel CC, Kooperberg C, Bartholomew B, Tsukiyama T. Yeast Isw1p forms two separable complexes in vivo. Mol Cell Biol 23(1):80-91, 2003. PMID: 12482963.
- Kassabov SR, Henry NM, Zofall M, Tsukiyama T, Bartholomew B. High-resolution mapping of changes in histone-DNA contacts of nucleosomes remodeled by ISW2. Mol Cell Biol 22(21):7524-34, 2002. PMID: 12370299.
- Sengupta SM, VanKanegan M, Persinger J, Logie C, Cairns BR, Peterson CL, Bartholomew B. The interactions of yeast SWI/SNF and RSC with the nucleosome before and after chromatin remodeling. J Biol Chem 276(16):12636-44, 2001. PMID: 11304548.
- Persinger J, Bartholomew B. Site-directed DNA photoaffinity labeling of RNA polymerase III transcription complexes. Methods Mol Biol 148:363-81, 2001. PMID: 11357598.
- Zofall M, Bartholomew B. Two novel dATP analogs for DNA photoaffinity labeling. Nucleic Acids Res 28(21):4382-90, 2000. PMID: 11058139.
- Michelson RJ, Collard MW, Ziemba AJ, Persinger J, Bartholomew B, Huggenvik JI. Nuclear DEAF-1-related (NUDR) protein contains a novel DNA binding domain and represses transcription of the heterogeneous nuclear ribonucleoprotein A2/B1 promoter. J Biol Chem 274(43):30510-9, 1999. PMID: 10521432.
- Persinger J, Sengupta SM, Bartholomew B. Spatial organization of the core region of yeast TFIIIB-DNA complexes. Mol Cell Biol 19(7):5218-34, 1999. PMID: 10373570.
- Tate JJ, Persinger J, Bartholomew B. Survey of four different photoreactive moieties for DNA photoaffinity labeling of yeast RNA polymerase III transcription complexes. Nucleic Acids Res 26(6):1421-6, 1998. PMID: 9490787.
- Persinger J, Bartholomew B. Mapping the contacts of yeast TFIIIB and RNA polymerase III at various distances from the major groove of DNA by DNA photoaffinity labeling. J Biol Chem 271(51):33039-46, 1996. PMID: 8955150.
- Pruss D, Bartholomew B, Persinger J, Hayes J, Arents G, Moudrianakis EN, Wolffe AP. An asymmetric model for the nucleosome: a binding site for linker histones inside the DNA gyres. Science 274(5287):614-7, 1996. PMID: 8849453.
- Lannutti BJ, Persinger J, Bartholomew B. Probing the protein-DNA contacts of a yeast RNA polymerase III transcription complex in a crude extract: solid phase synthesis of DNA photoaffinity probes containing a novel photoreactive deoxycytidine analog. Biochemistry 35(30):9821-31, 1996. PMID: 8703956.
- Bartholomew B, Tinker RL, Kassavetis GA, Geiduschek EP. Photochemical cross-linking assay for DNA tracking by replication proteins. Methods Enzymol 262:476-94, 1995. PMID: 8594372.
- Bartholomew B, Braun BR, Kassavetis GA, Geiduschek EP. Probing close DNA contacts of RNA polymerase III transcription complexes with the photoactive nucleoside 4-thiodeoxythymidine. J Biol Chem 269(27):18090-5, 1994. PMID: 8027070.
- Bartholomew B, Durkovich D, Kassavetis GA, Geiduschek EP. Orientation and topography of RNA polymerase III in transcription complexes. Mol Cell Biol 13(2):942-52, 1993. PMID: 8423814.
- Braun BR, Bartholomew B, Kassavetis GA, Geiduschek EP. Topography of transcription factor complexes on the Saccharomyces cerevisiae 5 S RNA gene. J Mol Biol 228(4):1063-77, 1992. PMID: 1474578.
- Bartholomew B, Kassavetis GA, Geiduschek EP. Two components of Saccharomyces cerevisiae transcription factor IIIB (TFIIIB) are stereospecifically located upstream of a tRNA gene and interact with the second-largest subunit of TFIIIC. Mol Cell Biol 11(10):5181-9, 1991. PMID: 1922038.
- Kassavetis GA, Bartholomew B, Blanco JA, Johnson TE, Geiduschek EP. Two essential components of the Saccharomyces cerevisiae transcription factor TFIIIB: transcription and DNA-binding properties. Proc Natl Acad Sci U S A 88(16):7308-12, 1991. PMID: 1871137.
- Bartholomew B, Kassavetis GA, Braun BR, Geiduschek EP. The subunit structure of Saccharomyces cerevisiae transcription factor IIIC probed with a novel photocrosslinking reagent. EMBO J 9(7):2197-205, 1990. PMID: 2100996.
- Bartholomew B, Meares CF, Dahmus ME. Photoaffinity labeling of RNA polymerase III transcription complexes by nascent RNA. J Biol Chem 265(7):3731-7, 1990. PMID: 2303478.
- Bartholomew B, Dahmus ME, Meares CF. RNA contacts subunits IIo and IIc in HeLa RNA polymerase II transcription complexes. J Biol Chem 261(30):14226-31, 1986. PMID: 2429953.
Invited Articles
- Paul S, Bartholomew B. Regulation of ATP-dependent chromatin remodelers: Accelerators/Brakes, Anchors and Sensors. Biochem Soc Trans 46(6):1423-1430, 2018. e-Pub 2018. PMID: 30467122.
- Meng H, Bartholomew B. Emerging Roles of Transcriptional Enhancers In Chromatin Looping And Promoter-Proximal Pausing Of RNA Polymerase II. J Biol Chem 293(36):13786-13794, 2018. e-Pub 2017. PMID: 29187597.
- Bartholomew B. Proteasomes beyond proteolysis: Roles in heterochromatin maintenance. J Biol Chem 292(41):17156-17157, 2017. PMID: 29030538.
- Bartholomew B. ISWI chromatin remodeling: one primary actor or a coordinated effort?. Curr Opin Struct Biol 24:150-5, 2014. e-Pub 2014. PMID: 24561830.
- Bartholomew B. Regulating the Chromatin Landscape: Structural and Mechanistic Perspectives. Annu Rev Biochem 83:671-96, 2014. e-Pub 2014. PMID: 24606138.
Other Articles
- Saha, D, Animireddy, S, Bartholomew, B The SWI/SNF ATP-dependent chromatin remodeling complex in cell lineage priming and early development. Biochemical Society Transactions 52(2):603-616, 2024. PMID: 38572912.
- Bartholomew B, Shukla S, Ngubo M, Paul S, Persinger J, Brahma S New insights into the mechanism and DNA-sequence specificity of INO80 chromatin remodeling. Res Sq Preprint, 2023. PMID: 37961512.
- Saha D, Animireddy S, Lee J, Thommen A, McKenzie M, Lu Y, Calabrese M, Bartholomew B Super-Enhancer Swithing in Early Mammalian Development Involves eRNA Mediated SWI/SNF Recruitment. SneakPeak Prepring, 2023. PMID: None.
- Hota SK, Bartholomew B Diversity of operation in ATP-dependent chromatin remodelers. Biochim Biophys Acta 1809(9):476-87, 2011. PMID: 21616185.
- Gangaraju VK, Bartholomew B Mechanisms of ATP dependent chromatin remodeling. Mutat Res 618(1-2):3-17, 2007. PMID: 17306844.
- Sengupta SM, Persinger J, Bartholomew B, Peterson CL Use of DNA photoaffinity labeling to study nucleosome remodeling by SWI/SNF. Methods 19(3):434-46, 1999. PMID: 10579939.
Book Chapters
- Olefumi L, Bartholomew B. Chromatin Remodeling. In: Encyclopedia of Genetics 2nd Edition. None. None, 2012.
- Sen P, Chatterjee N, and Bartholomew B. SWI/SNF Chromatin Remodeling Complex. In: Encyclopedia of Signaling Molecules. None. Springer Publishers, 2011.
- Persinger J, Bartholomew B. DNA-Protein Interactions: Principles and Protocols. In: 3rd Edition: Site-directed DNA Crosslinking of Large Multi-subunit Protein-DNA Complexes. None. None, 2009.
- Chatterjee N, Sen P, Bartholomew B. The SWI/SNF and RSC Nucleosome Remodeling Complexes. In: Handbook of Cell Signaling. None. Elsevier Inc, 2008.
- Kassabov SK, and Bartholomew B. Site-Directed Histone-DNA Contact mapping for Analysis of Nucleosome Dynamics High-resolution Mapping of Changes in Histone-DNA Contacts of Nucleosomes Remodeled by ISW2. In: Chromatin and Chromatin Remodeling. None. None, 2004.
- Persinger J, Bartholomew B. Site-specific DNA photoaffinity labeling of RNA polymerase III transcription complexes. In: Methods in Molecular Biology: Protein-DNA Interaction Protocols. None. None, 2000.
- Kassavetis GA, Bardeleben C, Bartholomew B, Braun BR, Joazeiro CA, Pisano M, Geiduschek EP. Transcription by RNA Polymerase III. In: Transcription Mechanisms and Regulation. None. None, 1994.
Grant & Contract Support
| Title: | Regulation of sub-TAD organization and enhancer-promoter interactions by SWI/SNF and its AT-hook |
| Funding Source: | Internal/MD Anderson |
| Role: | PI |
| Title: | Regulation of chromatin organization and dynamics by INO80 |
| Funding Source: | NIH/NIGMS |
| Role: | PI |
| Title: | Regulation of Transcription Hubs and Condensates by SWI/SNF and Non-coding RNA in Neural Development |
| Funding Source: | NIH/NIGMS |
| Role: | PI |
| Title: | Regulation of Chromatin Organization and Dynamics by INO80 |
| Funding Source: | The University of Texas MD Anderson Cancer Center |
| Role: | PI |
| Title: | Regulation of Transcription and Nuclear Architecture by SWI/SNF |
| Funding Source: | NIH |
| Role: | PI |
| Title: | Mechanism of INO80 Remodeling |
| Funding Source: | Internal/MD Anderson Cancer Center |
| Role: | PI |
| Title: | Regulation of RNA polymerase II pausing and directionality by ATP-dependent chromatin remodelers |
| Funding Source: | NIH/NIGMS |
| Role: | PI |
| Title: | Structure and Function of INO80: INO80's role in transcription and centromere formation |
| Funding Source: | UTMDACC |
| Role: | PI |
| Title: | NO TITLE PROVIDED |
| Funding Source: | UTMDACC |
| Role: | PI |
| Title: | The roles and mechanisms of CHD7 and CHD8 in mammalian development |
| Funding Source: | UTMDACC |
| Role: | PI |
| Title: | Examination of the function and structure of BRG1, ARID1A and 1B using a CRISPR-Cas9 based screen |
| Funding Source: | MD Anderson Cancer Center |
| Role: | PI |
| Title: | The interplay between the chromatin remodeler INO80 and histone variant H2A.Z |
| Funding Source: | NIH/NIGMS |
| Role: | PI |
| Title: | Attacking Cancer through Nobel Chromatin-based Pathways Regulating Genome Stability |
| Funding Source: | MD Anderson Cancer Center |
| Role: | Co-I |
| Title: | Role of the SnAC and AT hook domain in intestinal cancer |
| Funding Source: | MD Anderson Cancer Center |
| Role: | Principal Investigator-MDACC |
| Title: | Transcriptional Activation by Reorganizing Chromatin |
| Funding Source: | NIH/NIGMS |
| Role: | Principal Investigator-MDACC |
| Title: | Chromatin Remodeling and Transcription Repression |
| Funding Source: | NIH/NIGMS |
| Role: | PI |
| Title: | Chromatin Remodeling and Transcription Repression |
| Funding Source: | NIH/NIGMS |
| Role: | PI |
| Title: | Dynamics of Higher Order Chromatin Structure |
| Funding Source: | National Science Foundation (NSF) |
| Role: | PI |
| Title: | Chromatin Remodeling by the ISWI complexes from Yeast |
| Funding Source: | American Cancer Society (ACS) |
| Role: | PI |
| Title: | X-Ray Scattering |
| Funding Source: | Synchrotron Radiation |
| Role: | Co-I |
| Title: | Transcriptional Activation by Reorganizing Chromatin |
| Funding Source: | NIH/NIGMS |
| Role: | PI |
| Title: | Mechanism of RNA polymerase III Recognition |
| Funding Source: | NIH/NIGMS |
| Role: | PI |
| Title: | Targeting super-enhancer activation by eRNA mediated recruitment of SWI/SNF |
| Funding Source: | Cancer Prevention & Research Institute of Texas (CPRIT) |
| Role: | PI |
| Title: | The role of the ARID1A and SMARCD1 subunits of SWI/SNF in cancer |
| Funding Source: | UTMDACC |
| Role: | PI |
| Title: | Structure and Function of INO80: INO80’s role in transcription and centromere formation |
| Funding Source: | NIH/NIGMS |
| Role: | PI |
| Title: | SWI/SNF, RNA polymerase directionality/pausing and cancer |
| Funding Source: | Cancer Prevention & Research Institute of Texas (CPRIT) |
| Role: | PI |
| Title: | Transcription Activation by Reorganizing Chromatin |
| Funding Source: | NIH/NIGMS |
| Role: | Principal Investigator-MDACC |
| Title: | Epigenetic mechanism of ARID1A in pancreatic cancer |
| Funding Source: | Cancer Prevention & Research Institute of Texas (CPRIT) |
| Role: | Principal Investigator-MDACC |
| Title: | Architecture and conformation specific ChIP-seq |
| Funding Source: | NIH/NIBIB |
| Role: | Co-PI |
| Title: | Aberrant SWI/SNF and pediatric cancer |
| Funding Source: | Cancer Prevention & Research Institute of Texas (CPRIT) |
| Role: | PI |
| Title: | SWI/SNF, 3D genome, and Cancer |
| Funding Source: | Cancer Prevention & Research Institute of Texas (CPRIT) |
| Role: | Investigator |
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CV information above last modified September 20, 2024