Assistant Professor, Department of Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, Smithville, TX
Compromised DNA repair is a common feature of cancers and contributes to loss of genome integrity and tumorigenesis. A critical mechanism to faithfully repair DNA lesions, especially double-strand breaks, is homologous recombination. While many homologous recombination pathway components have been identified, their in vivo roles, especially within mammalian genomic contexts are poorly understood. Research in my laboratory uses genetic and molecular techniques to determine how homologous recombination pathways collaborate, compete, and compensate for one another. In particular, we use newly developed assay systems that take advantage of meiotic recombination in mouse spermatocytes to comprehensively dissect at high-resolution recombination outcomes. We plan to leverage this approach to (1) define the in vivo molecular characteristics of independent DNA repair pathways (2) discover new components of these pathways (3) provide a means of testing chemotherapeutic agents to specifically perturb individual pathways with a hope to improve cancer therapies.
|2003||Mount Sinai School of Medicine of New York University, New York, NY, USA, PHD, Biomedical Sciences|
|1992||Hunter College of the City University of New York, New York, NY, USA, BA, Biological Sciences|
|2004-2012||Research Fellowship, Memorial Sloan-Kettering Cancer Center, New York, NY|
|2003-2004||Research Fellowship, Mount Sinai School of Medicine, New York, NY|
|2015||NIH Director's New Innovator awardee, NIH Common Fund|
|2014||R. Lee Clark Fellow, The University of Texas MD Anderson Cancer Center|
|2012||CPRIT Scholar in Cancer Research, Cancer Prevention and Research Institute of Texas|
- Hansen RK, Mund A, Poulsen SL, Sandoval M, Klement K, Tsouroula K, Tollenaere MA, Räschle M, Soria R, Offermanns S, Worzfeld T, Grosse R, Brandt DT, Rozell B, Mann M, Cole F, Soutoglou E, Goodarzi AA, Daniel JA, Mailand N, Bekker-Jensen S. SCAI promotes DNA double-strand break repair in distinct chromosomal contexts. Nat Cell Biol 18 (12) :1357-1366, 2016. PMID: 27820601.
- Li M, Cole F, Patel DS, Misenko SM, Her J, Malhowski A, Alhamza A, Zheng H, Baer R, Ludwig T, Jasin M, Nussenzweig A, Serrano L, Bunting SF. 53BP1 Ablation Rescues Genomic Instability In Mice Expressing 'RING-less' BRCA1. EMBO Rep 17 (11) :1532-1541, 2016. PMID: 27670884.
- Cole F, Baudat F, Grey C, Keeney S, de Massy B, Jasin M. Mouse tetrad analysis provides insights into recombination mechanisms and hotspot evolutionary dynamics. Nat Genet 46 (10) :1072-80, 2014. PMID: 25151354.
- Cole F, Kauppi L, Lange J, Roig I, Wang R, Keeney S, Jasin M. Homeostatic control of recombination is implemented progressively in mouse meiosis. Nat Cell Biol 14 (4) :424-30, 2012. PMID: 22388890.
- Zhang W, Kang JS, Cole F, Yi MJ, Krauss RS. Cdo functions at multiple points in the Sonic Hedgehog pathway, and Cdo-deficient mice accurately model human holoprosencephaly. Dev Cell 10 (5) :657-65, 2006. PMID: 16647303.
- Tenzen T, Allen BL, Cole F, Kang JS, Krauss RS, McMahon AP. The cell surface membrane proteins Cdo and Boc are components and targets of the Hedgehog signaling pathway and feedback network in mice. Dev Cell 10 (5) :647-56, 2006. PMID: 16647304.
- Zhang W, Yi MJ, Chen X, Cole F, Krauss RS, Kang JS. Cortical thinning and hydrocephalus in mice lacking the immunoglobulin superfamily member CDO. Mol Cell Biol 26 (10) :3764-72, 2006. PMID: 16648472.
- Cole F, Zhang W, Geyra A, Kang JS, Krauss RS. Positive regulation of myogenic bHLH factors and skeletal muscle development by the cell surface receptor CDO. Dev Cell 7 (6) :843-54, 2004. PMID: 15572127.
- Zelazowski MJ, Sandoval M, Paniker L, Hamilton H, Han J, Gribbell MA, Kang R, Cole F. Age-dependent alterations in meiotic recombination cause chromosome segregation errors in spermatocytes. Cell.
- Takata KI, Reh S, Yousefzadeh MJ, Zelazowski MJ, Bhetawal S, Trono D, Lowery MG, Sandoval M, Takata Y, Lu Y, Lin K, Shen J, Kusewitt DF, McBride KM, Cole F, Wood RD. Analysis of DNA polymerase ν function in meiotic recombination, immunoglobulin class-switching, and DNA damage tolerance. PLoS Genet 13 (6) :e1006818. e-Pub 2017. PMID: 28570559.
- Vélez-Cruz R, Manickavinayaham S, Biswas AK, Clary RW, Premkumar T, Cole F, Johnson DG. RB localizes to DNA double-strand breaks and promotes DNA end resection and homologous recombination through the recruitment of BRG1. Genes Dev 30 (22) :2500-2512. PMID: 27940962.
- Shakya R, Reid LJ, Reczek CR, Cole F, Egli D, Lin CS, deRooij DG, Hirsch S, Ravi K, Hicks JB, Szabolcs M, Jasin M, Baer R, Ludwig T. BRCA1 tumor suppression depends on BRCT phosphoprotein binding, but not its E3 ligase activity. Science 334 (6055) :525-8. PMID: 22034435.
- Kang JS, Yi MJ, Zhang W, Feinleib JL, Cole F, Krauss RS. Netrins and neogenin promote myotube formation. J Cell Biol 167 (3) :493-504. PMID: 15520228.
- Cole F, Krauss RS. Microform holoprosencephaly in mice that lack the Ig superfamily member Cdon. Curr Biol 13 (5) :411-5. PMID: 12620190.
- Lange J, Pan J, Cole F, Thelen MP, Jasin M, Keeney S. ATM controls meiotic double-strand-break formation. Nature 479 (7372) :237-40. PMID: 22002603.
- Cole F, Keeney S, Jasin M. Comprehensive, fine-scale dissection of homologous recombination outcomes at a hot spot in mouse meiosis. Mol Cell 39 (5) :700-10. PMID: 20832722.
- Zelazowski MJ, Cole F. X marks the spot: PRDM9 rescues hybrid sterility by finding hidden treasure in the genome. Nat Struct Mol Biol 23 (4) :267-9, 2016. PMID: 27045445.
- Cole F. The sense and sensibility of strand exchange in recombination homeostasis. PLoS Genet 10 (1) :e1004104, 2014. PMID: 24465222.
- Cole F, Keeney S, Jasin M. Preaching about the converted: how meiotic gene conversion influences genomic diversity. Ann N Y Acad Sci 1267 (1) :95-102, 2012. PMID: 22954222.
- Cole F, Keeney S, Jasin M. Evolutionary conservation of meiotic DSB proteins: more than just Spo11. Genes Dev 24 (12) :1201-7, 2010. PMID: 20551169.
- Bergemann AD, Cole F, Hirschhorn K. The etiology of Wolf-Hirschhorn syndrome. Trends Genet 21 (3) :188-95, 2005. PMID: 15734578.
- Krauss RS, Cole F, Gaio U, Takaesu G, Zhang W, Kang JS. Close encounters: regulation of vertebrate skeletal myogenesis by cell-cell contact. J Cell Sci 118 (Pt 11) :2355-62, 2005. PMID: 15923648.
- Cole F, Jasin M. Isolation of meiotic recombinants from mouse sperm. In: Methods Mol Biol, 251-82, 2011.
|Title:||Recruitment of First-Time, Tenure-Track Faculty Members|
|Funding Source:||Cancer Prevention & Research Institute of Texas (CPRIT)|
|Title:||Start up funds|
|Funding Source:||MD Anderson Cancer Center|
|Title:||Mechanisms of mutagenesis|
|Funding Source:||MD Anderson Cancer Center|
|Title:||Mechanistic derivation of germ line mutation by genome-wide mouse tetrad analysis|
|Title:||Determine how tobacco exposure influences meiotic recombination integrity|
|Funding Source:||Tobacco Pilot Award, The University of Texas MD Anderson Cancer Center|