Traver Hart, Ph.D.
Department of Bioinformatics and Computational Biology, Division of Basic Sciences
Present Title & Affiliation
Primary Appointment
Assistant Professor, Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center, Houston, TX
Dual/Joint/Adjunct Appointment
Assistant Professor, Department of Cancer Biology, The University of Texas MD Anderson Cancer Center, Houston, TX
Education & Training
Degree-Granting Education
| 2008 | The University of Texas at Austin, Austin, TX, USA, PHD, Cell & Molecular Biology |
| 2003 | University of Texas at Austin, Austin, TX, USA, BA, Middle Eastern Studies |
Postgraduate Training
| 2012-2015 | Postdoctoral Fellow, University of Toronto, Toronto |
| 2009-2012 | Postdoctoral Fellow, Genomics/Immunology, The Scripps Research institute, La Jolla, CA |
Selected Publications
Peer-Reviewed Articles
- Noordermeer SM, Adam S, Setiaputra D, Barazas M, Pettitt SJ, Ling AK, Olivieri M, Álvarez-Quilón A, Moatti N, Zimmermann M, Annunziato S, Krastev DB, Song F, Brandsma I, Frankum J, Brough R, Sherker A, Landry S, Szilard RK, Munro MM, McEwan A, Goullet de Rugy T, Lin ZY, Hart T, Moffat J, Gingras AC, Martin A, van Attikum H, Jonkers J, Lord CJ, Rottenberg S, Durocher D. The Shieldin complex mediates 53BP1-dependent DNA repair. Nature 560(7716):117-121, 2018. e-Pub 2018. PMID: 30022168.
- Zimmermann M, Murina O, Reijns MAM, Agathanggelou A, Challis R, Tarnauskaite Ž, Muir M, Fluteau A, Aregger M, McEwan A, Yuan W, Clarke M, Lambros MB, Paneesha S, Moss P, Chandrashekhar M, Angers S, Moffat J, Brunton VG, Hart T, de Bono J, Stankovic T, Jackson AP, Durocher D. CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions. Nature 559(7713):285-289, 2018. e-Pub 2018. PMID: 29973717.
- Lenoir WF, Lim TL, Hart T. PICKLES: the database of pooled in-vitro CRISPR knockout library essentiality screens. Nucleic Acids Res 46(D1):D776-D780, 2018. PMID: 29077937.
- Hart T, Tong AHY, Chan K, Van Leeuwen J, Seetharaman A, Aregger M, Chandrashekhar M, Hustedt N, Seth S, Noonan A, Habsid A, Sizova O, Nedyalkova L, Climie R, Tworzyanski L, Lawson K, Sartori MA, Alibeh S, Tieu D, Masud S, Mero P, Weiss A, Brown KR, Usaj M, Billmann M, Rahman M, Constanzo M, Myers CL, Andrews BJ, Boone C, Durocher D, Moffat J. Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens. G3 (Bethesda) 7(8):2719-2727, 2017. e-Pub 2017. PMID: 28655737.
- Steinhart Z, Pavlovic Z, Chandrashekhar M, Hart T, Wang X, Zhang X, Robitaille M, Brown KR, Jaksani S, Overmeer R, Boj SF, Adams J, Pan J, Clevers H, Sidhu S, Moffat J, Angers S. Genome-wide CRISPR screens reveal a Wnt-FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors. Nat Med 23(1):60-68, 2017. e-Pub 2016. PMID: 27869803.
- Hart T, Moffat J. BAGEL: a computational framework for identifying essential genes from pooled library screens. BMC Bioinformatics 17:164, 2016. e-Pub 2016. PMID: 27083490.
- Hart T, Chandrashekhar M, Aregger M, Steinhart Z, Brown KR, MacLeod G, Mis M, Zimmermann M, Fradet-Turcotte A, Sun S, Mero P, Dirks P, Sidhu S, Roth FP, Rissland OS, Durocher D, Angers S, Moffat J. High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Cell 163(6):1515-26, 2015. e-Pub 2015. PMID: 26627737.
- Toledo CM, Ding Y, Hoellerbauer P, Davis RJ, Basom R, Girard EJ, Lee E, Corrin P, Hart T, Bolouri H, Davison J, Zhang Q, Hardcastle J, Aronow BJ, Plaisier CL, Baliga NS, Moffat J, Lin Q, Li XN, Nam DH, Lee J, Pollard SM, Zhu J, Delrow JJ, Clurman BE, Olson JM, Paddison PJ. Genome-wide CRISPR-Cas9 Screens Reveal Loss of Redundancy between PKMYT1 and WEE1 in Glioblastoma Stem-like Cells. Cell Rep 13(11):2425-39, 2015. e-Pub 2015. PMID: 26673326.
- Pandyra AA, Mullen PJ, Goard CA, Ericson E, Sharma P, Kalkat M, Yu R, Pong JT, Brown KR, Hart T, Gebbia M, Lang KS, Giaever G, Nislow C, Moffat J, Penn LZ. Genome-wide RNAi analysis reveals that simultaneous inhibition of specific mevalonate pathway genes potentiates tumor cell death. Oncotarget 6(29):26909-21, 2015. PMID: 26353928.
- Vu V, Verster AJ, Schertzberg M, Chuluunbaatar T, Spensley M, Pajkic D, Hart GT, Moffat J, Fraser AG. Natural Variation in Gene Expression Modulates the Severity of Mutant Phenotypes. Cell 162(2):391-402, 2015. PMID: 26186192.
- Hart T, Brown KR, Sircoulomb F, Rottapel R, Moffat J. Measuring error rates in genomic perturbation screens: gold standards for human functional genomics. Mol Syst Biol 10:733, 2014. e-Pub 2014. PMID: 24987113.
- Havugimana PC, Hart GT, Nepusz T, Yang H, Turinsky AL, Li Z, Wang PI, Boutz DR, Fong V, Phanse S, Babu M, Craig SA, Hu P, Wan C, Vlasblom J, Dar VU, Bezginov A, Clark GW, Wu GC, Wodak SJ, Tillier ER, Paccanaro A, Marcotte EM, Emili A. A census of human soluble protein complexes. Cell 150(5):1068-81, 2012. PMID: 22939629.
- Narayanaswamy R, Moradi EK, Niu W, Hart GT, Davis M, McGary KL, Ellington AD, Marcotte EM. Systematic definition of protein constituents along the major polarization axis reveals an adaptive reuse of the polarization machinery in pheromone-treated budding yeast. J Proteome Res 8(1):6-19, 2009. PMID: 19053807.
- Ramani AK, Li Z, Hart GT, Carlson MW, Boutz DR, Marcotte EM. A map of human protein interactions derived from co-expression of human mRNAs and their orthologs. Mol Syst Biol 4:180, 2008. e-Pub 2008. PMID: 18414481.
- Hart GT, Lee I, Marcotte ER. A high-accuracy consensus map of yeast protein complexes reveals modular nature of gene essentiality. BMC Bioinformatics 8:236, 2007. e-Pub 2007. PMID: 17605818.
- Hart GT, Ramani AK, Marcotte EM. How complete are current yeast and human protein-interaction networks?. Genome Biol 7(11):120, 2006. PMID: 17147767.
- Wang C, Wang G, Feng X, Shepherd P, Zhang J, Tang M, Chen Z, Srivastava M, McLaughlin ME, Navone NM, Hart GT, Chen J. Genome-wide CRISPR screens reveal synthetic lethality of RNASEH2 deficiency and ATR inhibition. Oncogene. e-Pub 2018. PMID: 30532030.
- Hwang S, Kim CY, Yang S, Kim E, Hart T, Marcotte EM, Lee I. HumanNet v2: human gene networks for disease research. Nucleic Acids Res. e-Pub 2018. PMID: 30418591.
Grant & Contract Support
| Title: | Recruitment of First-time Tenure-Track Faculty |
| Funding Source: | Cancer Prevention & Research Institute of Texas (CPRIT) |
| Role: | Principal Investigator |
| Title: | EXPLOITING PATTERNS OF GENE ESSENTIALITY IN HUMAN CELLS TO PREDICT GENE FUNCTION, SYNTHETIC LETHALITY, AND CANCER TARGETS |
| Funding Source: | NIH/NIGMS |
| Role: | Principal Investigator |
| Title: | Synthetic essential approach to identify novel therapeutic targets for prostate cancer |
| Funding Source: | Prostate Cancer Foundation |
| Role: | Co-Investigator |
| Title: | Characterizing and targeting intra-tumor heterogeneity in triple negative breast cancer chemoresistance and multi-organ metastasis |
| Funding Source: | NIH/NCI |
| Role: | Mentor |