Traver Hart, Ph.D.
Department of Bioinformatics and Computational Biology, Division of Basic Sciences
Present Title & Affiliation
Primary Appointment
Assistant Professor, Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center, Houston, TX
Dual/Joint/Adjunct Appointment
Assistant Professor, Department of Cancer Biology, The University of Texas MD Anderson Cancer Center, Houston, TX
Education & Training
Degree-Granting Education
| 2008 | The University of Texas at Austin, Austin, TX, USA, PHD, Cell & Molecular Biology |
| 2003 | University of Texas at Austin, Austin, TX, USA, BA, Middle Eastern Studies |
Postgraduate Training
| 2012-2015 | Postdoctoral Fellow, Cancer Functional Genomics, University of Toronto, Toronto |
| 2009-2012 | Postdoctoral Fellow, Genomics/Immunology, The Scripps Research institute, La Jolla, CA |
Selected Publications
Peer-Reviewed Articles
- Lenoir WF, Morgado M, DeWeirdt PC, McLaughlin M, Griffith AL, Sangree AK, Feeley MN, Esmaeili Anvar N, Kim E, Bertolet LL, Colic M, Dede M, Doench JG, Hart T. Discovery of putative tumor suppressors from CRISPR screens reveals rewired lipid metabolism in acute myeloid leukemia cells. Nat Commun 12(1):6506, 2021. e-Pub 2021. PMID: 34764293.
- Yoshihama Y, LaBella KA, Kim E, Bertolet L, Colic M, Li J, Shang X, Wu CJ, Spring DJ, Wang YA, Hart T, DePinho RA. AR-negative prostate cancer is vulnerable to loss of JMJD1C demethylase. Proc Natl Acad Sci U S A 118(36), 2021. PMID: 34475205.
- Wang C, Tang M, Chen Z, Nie L, Li S, Xiong Y, Szymonowicz KA, Park JM, Zhang H, Feng X, Huang M, Su D, Hart T, Chen J. Genetic vulnerabilities upon inhibition of DNA damage response. Nucleic Acids Res 49(14):8214-8231, 2021. e-Pub 2021. PMID: 34320214.
- Kim E, Hart T. Improved analysis of CRISPR fitness screens and reduced off-target effects with the BAGEL2 gene essentiality classifier. Genome Med 13(1):2, 2021. e-Pub 2021. PMID: 33407829.
- Wang R, Lenoir WF, Wang C, Su D, McLaughlin M, Hu Q, Shen X, Tian Y, Klages-Mundt N, Lynn E, Wood RD, Chen J, Hart T, Li L. DNA polymerase ι compensates for Fanconi anemia pathway deficiency by countering DNA replication stress. Proc Natl Acad Sci U S A 117(52):33436-33445, 2020. e-Pub 2020. PMID: 33376220.
- Wang C, Chen Z, Su D, Tang M, Nie L, Zhang H, Feng X, Wang R, Shen X, Srivastava M, McLaughlin ME, Hart T, Li L, Chen J. C17orf53 is identified as a novel gene involved in inter-strand crosslink repair. DNA Repair (Amst) 95:102946, 2020. e-Pub 2020. PMID: 32853826.
- Lawson KA, Sousa CM, Zhang X, Kim E, Akthar R, Caumanns JJ, Yao Y, Mikolajewicz N, Ross C, Brown KR, Zid AA, Fan ZP, Hui S, Krall JA, Simons DM, Slater CJ, De Jesus V, Tang L, Singh R, Goldford JE, Martin S, Huang Q, Francis EA, Habsid A, Climie R, Tieu D, Wei J, Li R, Tong AHY, Aregger M, Chan KS, Han H, Wang X, Mero P, Brumell JH, Finelli A, Ailles L, Bader G, Smolen GA, Kingsbury GA, Hart T, Kung C, Moffat J. Functional genomic landscape of cancer-intrinsic evasion of killing by T cells. Nature 586(7827):120-126, 2020. e-Pub 2020. PMID: 32968282.
- Dede M, McLaughlin M, Kim E, Hart T. Multiplex enCas12a screens detect functional buffering among paralogs otherwise masked in monogenic Cas9 knockout screens. Genome Biol 21(1):262, 2020. e-Pub 2020. PMID: 33059726.
- Aubert L, Nandagopal N, Steinhart Z, Lavoie G, Nourreddine S, Berman J, Saba-El-Leil MK, Papadopoli D, Lin S, Hart T, Macleod G, Topisirovic I, Gaboury L, Fahrni CJ, Schramek D, Meloche S, Angers S, Roux PP. Copper bioavailability is a KRAS-specific vulnerability in colorectal cancer. Nat Commun 11(1):3701, 2020. e-Pub 2020. PMID: 32709883.
- Huang M, Feng X, Su D, Wang G, Wang C, Tang M, Paulucci-Holthauzen A, Hart T, Chen J. Genome-wide CRISPR screen uncovers a synergistic effect of combining Haspin and Aurora kinase B inhibition. Oncogene 39(21):4312-4322, 2020. e-Pub 2020. PMID: 32300176.
- Wertz MH, Mitchem MR, Pineda SS, Hachigian LJ, Lee H, Lau V, Powers A, Kulicke R, Madan GK, Colic M, Therrien M, Vernon A, Beja-Glasser VF, Hegde M, Gao F, Kellis M, Hart T, Doench JG, Heiman M. Genome-wide In Vivo CNS Screening Identifies Genes that Modify CNS Neuronal Survival and mHTT Toxicity. Neuron 106(1):76-89.e8, 2020. e-Pub 2020. PMID: 32004439.
- Su D, Feng X, Colic M, Wang Y, Zhang C, Wang C, Tang M, Hart T, Chen J. CRISPR/CAS9-based DNA damage response screens reveal gene-drug interactions. DNA Repair (Amst) 87:102803, 2020. e-Pub 2020. PMID: 31991288.
- Mis M, O'Brien S, Steinhart Z, Lin S, Hart T, Moffat J, Angers S. IPO11 mediates βcatenin nuclear import in a subset of colorectal cancers. J Cell Biol 219(2), 2020. PMID: 31881079.
- Hustedt N, Álvarez-Quilón A, McEwan A, Yuan JY, Cho T, Koob L, Hart T, Durocher D. A consensus set of genetic vulnerabilities to ATR inhibition. Open Biol 9(9):190156, 2019. e-Pub 2019. PMID: 31506018.
- Colic M, Wang G, Zimmermann M, Mascall K, McLaughlin M, Bertolet L, Lenoir WF, Moffat J, Angers S, Durocher D, Hart T. Identifying chemogenetic interactions from CRISPR screens with drugZ. Genome Med 11(1):52, 2019. e-Pub 2019. PMID: 31439014.
- Liu J, Srinivasan S, Li CY, Ho IL, Rose J, Shaheen M, Wang G, Yao W, Deem A, Bristow C, Hart T, Draetta G. Pooled library screening with multiplexed Cpf1 library. Nat Commun 10(1):3144, 2019. e-Pub 2019. PMID: 31316073.
- Wang C, Wang G, Feng X, Shepherd P, Zhang J, Tang M, Chen Z, Srivastava M, McLaughlin ME, Navone NM, Hart GT, Chen J. Genome-wide CRISPR screens reveal synthetic lethality of RNASEH2 deficiency and ATR inhibition. Oncogene 38(14):2451-2463, 2019. e-Pub 2018. PMID: 30532030.
- Kim E, Dede M, Lenoir WF, Wang G, Srinivasan S, Colic M, Hart T. A network of human functional gene interactions from knockout fitness screens in cancer cells. Life Sci Alliance 2(2), 2019. e-Pub 2019. PMID: 30979825.
- MacLeod G, Bozek DA, Rajakulendran N, Monteiro V, Ahmadi M, Steinhart Z, Kushida MM, Yu H, Coutinho FJ, Cavalli FMG, Restall I, Hao X, Hart T, Luchman HA, Weiss S, Dirks PB, Angers S. Genome-Wide CRISPR-Cas9 Screens Expose Genetic Vulnerabilities and Mechanisms of Temozolomide Sensitivity in Glioblastoma Stem Cells. Cell Rep 27(3):971-986.e9, 2019. PMID: 30995489.
- Hwang S, Kim CY, Yang S, Kim E, Hart T, Marcotte EM, Lee I. HumanNet v2: human gene networks for disease research. Nucleic Acids Res 47(D1):D573-D580, 2019. e-Pub 2018. PMID: 30418591.
- Noordermeer SM, Adam S, Setiaputra D, Barazas M, Pettitt SJ, Ling AK, Olivieri M, Álvarez-Quilón A, Moatti N, Zimmermann M, Annunziato S, Krastev DB, Song F, Brandsma I, Frankum J, Brough R, Sherker A, Landry S, Szilard RK, Munro MM, McEwan A, Goullet de Rugy T, Lin ZY, Hart T, Moffat J, Gingras AC, Martin A, van Attikum H, Jonkers J, Lord CJ, Rottenberg S, Durocher D. The Shieldin complex mediates 53BP1-dependent DNA repair. Nature 560(7716):117-121, 2018. e-Pub 2018. PMID: 30022168.
- Zimmermann M, Murina O, Reijns MAM, Agathanggelou A, Challis R, Tarnauskaite Ž, Muir M, Fluteau A, Aregger M, McEwan A, Yuan W, Clarke M, Lambros MB, Paneesha S, Moss P, Chandrashekhar M, Angers S, Moffat J, Brunton VG, Hart T, de Bono J, Stankovic T, Jackson AP, Durocher D. CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions. Nature 559(7713):285-289, 2018. e-Pub 2018. PMID: 29973717.
- Lenoir WF, Lim TL, Hart T. PICKLES: the database of pooled in-vitro CRISPR knockout library essentiality screens. Nucleic Acids Res 46(D1):D776-D780, 2018. PMID: 29077937.
- Hart T, Tong AHY, Chan K, Van Leeuwen J, Seetharaman A, Aregger M, Chandrashekhar M, Hustedt N, Seth S, Noonan A, Habsid A, Sizova O, Nedyalkova L, Climie R, Tworzyanski L, Lawson K, Sartori MA, Alibeh S, Tieu D, Masud S, Mero P, Weiss A, Brown KR, Usaj M, Billmann M, Rahman M, Constanzo M, Myers CL, Andrews BJ, Boone C, Durocher D, Moffat J. Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens. G3 (Bethesda) 7(8):2719-2727, 2017. e-Pub 2017. PMID: 28655737.
- Steinhart Z, Pavlovic Z, Chandrashekhar M, Hart T, Wang X, Zhang X, Robitaille M, Brown KR, Jaksani S, Overmeer R, Boj SF, Adams J, Pan J, Clevers H, Sidhu S, Moffat J, Angers S. Genome-wide CRISPR screens reveal a Wnt-FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors. Nat Med 23(1):60-68, 2017. e-Pub 2016. PMID: 27869803.
- Hart T, Moffat J. BAGEL: a computational framework for identifying essential genes from pooled library screens. BMC Bioinformatics 17:164, 2016. e-Pub 2016. PMID: 27083490.
- Hart T, Chandrashekhar M, Aregger M, Steinhart Z, Brown KR, MacLeod G, Mis M, Zimmermann M, Fradet-Turcotte A, Sun S, Mero P, Dirks P, Sidhu S, Roth FP, Rissland OS, Durocher D, Angers S, Moffat J. High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Cell 163(6):1515-26, 2015. e-Pub 2015. PMID: 26627737.
- Toledo CM, Ding Y, Hoellerbauer P, Davis RJ, Basom R, Girard EJ, Lee E, Corrin P, Hart T, Bolouri H, Davison J, Zhang Q, Hardcastle J, Aronow BJ, Plaisier CL, Baliga NS, Moffat J, Lin Q, Li XN, Nam DH, Lee J, Pollard SM, Zhu J, Delrow JJ, Clurman BE, Olson JM, Paddison PJ. Genome-wide CRISPR-Cas9 Screens Reveal Loss of Redundancy between PKMYT1 and WEE1 in Glioblastoma Stem-like Cells. Cell Rep 13(11):2425-39, 2015. e-Pub 2015. PMID: 26673326.
- Pandyra AA, Mullen PJ, Goard CA, Ericson E, Sharma P, Kalkat M, Yu R, Pong JT, Brown KR, Hart T, Gebbia M, Lang KS, Giaever G, Nislow C, Moffat J, Penn LZ. Genome-wide RNAi analysis reveals that simultaneous inhibition of specific mevalonate pathway genes potentiates tumor cell death. Oncotarget 6(29):26909-21, 2015. PMID: 26353928.
- Vu V, Verster AJ, Schertzberg M, Chuluunbaatar T, Spensley M, Pajkic D, Hart GT, Moffat J, Fraser AG. Natural Variation in Gene Expression Modulates the Severity of Mutant Phenotypes. Cell 162(2):391-402, 2015. PMID: 26186192.
- Hart T, Brown KR, Sircoulomb F, Rottapel R, Moffat J. Measuring error rates in genomic perturbation screens: gold standards for human functional genomics. Mol Syst Biol 10:733, 2014. e-Pub 2014. PMID: 24987113.
- Havugimana PC, Hart GT, Nepusz T, Yang H, Turinsky AL, Li Z, Wang PI, Boutz DR, Fong V, Phanse S, Babu M, Craig SA, Hu P, Wan C, Vlasblom J, Dar VU, Bezginov A, Clark GW, Wu GC, Wodak SJ, Tillier ER, Paccanaro A, Marcotte EM, Emili A. A census of human soluble protein complexes. Cell 150(5):1068-81, 2012. PMID: 22939629.
- Narayanaswamy R, Moradi EK, Niu W, Hart GT, Davis M, McGary KL, Ellington AD, Marcotte EM. Systematic definition of protein constituents along the major polarization axis reveals an adaptive reuse of the polarization machinery in pheromone-treated budding yeast. J Proteome Res 8(1):6-19, 2009. PMID: 19053807.
- Ramani AK, Li Z, Hart GT, Carlson MW, Boutz DR, Marcotte EM. A map of human protein interactions derived from co-expression of human mRNAs and their orthologs. Mol Syst Biol 4:180, 2008. e-Pub 2008. PMID: 18414481.
- Hart GT, Lee I, Marcotte ER. A high-accuracy consensus map of yeast protein complexes reveals modular nature of gene essentiality. BMC Bioinformatics 8:236, 2007. e-Pub 2007. PMID: 17605818.
- Hart GT, Ramani AK, Marcotte EM. How complete are current yeast and human protein-interaction networks?. Genome Biol 7(11):120, 2006. PMID: 17147767.
- Narayanaswamy R, Niu W, Scouras AD, Hart GT, Davies J, Ellington AD, Iyer VR, Marcotte EM. Systematic profiling of cellular phenotypes with spotted cell microarrays reveals mating-pheromone response genes. Genome Biol 7(1):R6, 2006. e-Pub 2006. PMID: 16507139.
- Kim CY, Baek S, Cha J, Yang S, Kim E, Marcotte EM, Hart T, Lee I. HumanNet v3: an improved database of human gene networks for disease research. Nucleic Acids Res. e-Pub 2021. PMID: 34747468.
- Eiru Kim, Lance C. Novak, Veronica Gheorghe, Christopher A. Bristow, Traver Hart. Dynamic rewiring of biological activity across genotype and lineage revealed by context-dependent functional interactions. bioRxiv.
Grant & Contract Support
| Title: | Exploiting Patterns of Gene Essentiality in Human Cells to Predict Gene Function, Synthetic Lethality, and Cancer Targets |
| Funding Source: | NIH/NIGMS |
| Role: | Principal Investigator |
| Title: | Characterizing and targeting intra-tumor heterogeneity in triple negative breast cancer chemoresistance and multi-organ metastasis |
| Funding Source: | NIH/NCI |
| Role: | Mentor |
| Title: | Identification of synthetic lethal and collateral lethal targets in prostate cancer |
| Funding Source: | Koch Foundation |
| Role: | Principal Investigator-MDACC |
| Title: | Multiplex CRISPR genetic modeling of polypharmacology for precision medicine |
| Funding Source: | Cancer Prevention & Research Institute of Texas (CPRIT) |
| Role: | Principal Investigator |