Meet George Eisenhoffer, Ph.D.
Assistant Professor, Department of Genetics, Division of Basic Science Research, The University of Texas MD Anderson Cancer Center, Houston, TX
Assistant Professor, The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, Houston, TX
Assistant Professor, Division of Developmental Program, Baylor College of Medicine Graduate School of Biomedical Sciences, Houston, TX
Zebrafish Development and Genetics Epithelial Tissue Homeostasis Regeneration Cell Extrusion
My lab focuses on understanding the coordination of cell division and death to control overall cell numbers in epithelia. Epithelial tissues provide an essential protective barrier for the organs they encase, and are the primary sites where most solid tumors or carcinomas form. Alterations in both cell loss and proliferation have been implicated in numerous human diseases, including cancer, yet our knowledge of how these two processes influence each other to regulate cell numbers within normal epithelia remains limited. The goal of our research is to elucidate the mechanisms that regulate epithelial cell turnover while preserving barrier function. Our work has uncovered that mechanical forces guide the inter-relationship between cell death and division during homeostatic cell turnover in epithelia, and that damage elicits a separate but equally important response. We have found that cell extrusion, a process used to eliminate cells from epithelia without disrupting barrier function, is the key to driving turnover in both scenarios. To investigate extrusion in a living epithelial tissue, we developed a cellular and molecular toolset to study the epidermis of developing zebrafish. This system provides unparalleled access to analyze epithelial cell turnover in vivo and in real time. We utilize a combinatorial approach that involves timelapse imaging and reverse genetic techniques to characterize cell turnover under physiological conditions, after damage, and when extrusion is perturbed to gain a better understanding of the specific alterations that lead to epithelial pathologies and cancer.
|2008||The University of Utah, Salt Lake City, UT, USA, PHD, Cell and Molecular Biology|
|1999||The University of Texas, Austin, TX, USA, BA, Microbiology|
|2009-2014||Postdoctoral Fellowship, Huntsman Cancer Institute, Salt Lake City, UT|
Institutional Committee Activities
Member, Faculty Academic Review Committee, 2014 - Present
- Brock CK, Wallin ST, Ruiz OE, Samms KM, Mandal A, Sumner EA, Eisenhoffer GT. Stem cell proliferation is induced by apoptotic bodies from dying cells during epithelial tissue maintenance. Nat Commun 10(1):1044, 2019. e-Pub 2019. PMID: 30837472.
- Fish JE, Cantu Gutierrez M, Dang LT, Khyzha N, Chen Z, Veitch S, Cheng HS, Khor M, Antounians L, Njock MS, Boudreau E, Herman AM, Rhyner AM, Ruiz OE, Eisenhoffer GT, Medina-Rivera A, Wilson MD, Wythe JD. Dynamic regulation of VEGF-inducible genes by an ERK-ERG-p300 transcriptional network. Development 144(13):2428-2444, 2017. e-Pub 2017. PMID: 28536097.
- Eisenhoffer GT, Slattum G, Ruiz OE, Otsuna H, Bryan CD, Lopez J, Wagner DS, Bonkowsky JL, Chien CB, Dorsky RI, Rosenblatt J. A toolbox to study epidermal cell types in zebrafish. J Cell Sci 130(1):269-277, 2017. e-Pub 2016. PMID: 27149923.
- Eisenhoffer GT, Rosenblatt J. Bringing balance by force: live cell extrusion controls epithelial cell numbers. Trends Cell Biol 23(4):185-92, 2013. e-Pub 2012. PMID: 23273931.
- Eisenhoffer GT, Loftus PD, Yoshigi M, Otsuna H, Chien CB, Morcos PA, Rosenblatt J. Crowding induces live cell extrusion to maintain homeostatic cell numbers in epithelia. Nature 484(7395):546-9, 2012. e-Pub 2012. PMID: 22504183.
- Eisenhoffer GT, Rosenblatt J. Live imaging of cell extrusion from the epidermis of developing zebrafish. J Vis Exp(52), 2011. e-Pub 2011. PMID: 21730948.
- Pearson BJ, Eisenhoffer GT, Gurley KA, Rink JC, Miller DE, Sánchez Alvarado A. Formaldehyde-based whole-mount in situ hybridization method for planarians. Dev Dyn 238(2):443-50, 2009. PMID: 19161223.
- Eisenhoffer GT, Kang H, Sánchez Alvarado A. Molecular analysis of stem cells and their descendants during cell turnover and regeneration in the planarian Schmidtea mediterranea. Cell Stem Cell 3(3):327-39, 2008. PMID: 18786419.
- Adey NB, Lei M, Howard MT, Jensen JD, Mayo DA, Butel DL, Coffin SC, Moyer TC, Slade DE, Spute MK, Hancock AM, Eisenhoffer GT, Dalley BK, McNeely MR. Gains in sensitivity with a device that mixes microarray hybridization solution in a 25-microm-thick chamber. Anal Chem 74(24):6413-7, 2002. PMID: 12510768.
- Franco J, Atieh Y, Bryan CD, Kwan KM, Eisenhoffer GT. Cellular Crowding Influences Extrusion and Proliferation to Facilitate Epithelial Tissue Repair. Mol Biol Cell:mbcE18050295. e-Pub 2019. PMID: 30785842.
- Rosa JT, Oralova V, Larionova D, Eisenhoffer GT, Eckhard W and Huysseune A. A. Periderm invasion contributes to epithelial formation in the teleost pharynx. Scientific Reports.
- Tatara AM, Wurster S, Lockworth CR, Albert ND, Walsh TJ, Mikos AG, Eisenhoffer GT, Kontoyiannis DP. Immunosuppressed Adult Zebrafish Model of Mucormycosis. Antimicrob Agents Chemother. e-Pub 2018. PMID: 30150461.
|Title:||The Role of Extrusion in Controlling Overall Numbers in Epithelia|
|Funding Source:||Cancer Prevention & Research Institute of Texas (CPRIT)|