Assistant Professor, Department of Genetics, Division of Basic Science Research, The University of Texas MD Anderson Cancer Center, Houston, TX
Zebrafish Development and Genetics Epithelial Tissue Homeostasis Regeneration Cell Extrusion
My lab focuses on understanding the coordination of cell division and death to control overall cell numbers in epithelia. Epithelial tissues provide an essential protective barrier for the organs they encase, and are the primary sites where most solid tumors or carcinomas form. Alterations in both cell loss and proliferation have been implicated in numerous human diseases, including cancer, yet our knowledge of how these two processes influence each other to regulate cell numbers within normal epithelia remains limited. The goal of our research is to elucidate the mechanisms that regulate epithelial cell turnover while preserving barrier function. Our work has uncovered that mechanical forces guide the inter-relationship between cell death and division during homeostatic cell turnover in epithelia, and that damage elicits a separate but equally important response. We have found that cell extrusion, a process used to eliminate cells from epithelia without disrupting barrier function, is the key to driving turnover in both scenarios. To investigate extrusion in a living epithelial tissue, we developed a cellular and molecular toolset to study the epidermis of developing zebrafish. This system provides unparalleled access to analyze epithelial cell turnover in vivo and in real time. We utilize a combinatorial approach that involves timelapse imaging and reverse genetic techniques to characterize cell turnover under physiological conditions, after damage, and when extrusion is perturbed to gain a better understanding of the specific alterations that lead to epithelial pathologies and cancer.
|2008||The University of Utah, Salt Lake City, UT, USA, PHD, Cell and Molecular Biology|
|1999||The University of Texas, Austin, TX, USA, BA, Microbiology|
|2009-2014||Postdoctoral Fellowship, Huntsman Cancer Institute, Salt Lake City, UT|
Member, Division of Developmental Program, Baylor College of Medicine Graduate School of Biomedical Sciences, Houston, TX, 2016 - Present
Member, Division of Genes and Development Program, The University of Texas Graduate School of Biomedical Sciences, Houston, TX, 2014 - Present
- Eisenhoffer GT, Rosenblatt J. Bringing balance by force: live cell extrusion controls epithelial cell numbers. Trends Cell Biol 23(4):185-92, 2013. e-Pub 2012. PMID: 23273931.
- Eisenhoffer GT, Loftus PD, Yoshigi M, Otsuna H, Chien CB, Morcos PA, Rosenblatt J. Crowding induces live cell extrusion to maintain homeostatic cell numbers in epithelia. Nature 484(7395):546-9, 2012. e-Pub 2012. PMID: 22504183.
- Eisenhoffer GT, Rosenblatt J. Live imaging of cell extrusion from the epidermis of developing zebrafish. J Vis Exp(52), 2011. e-Pub 2011. PMID: 21730948.
- Pearson BJ, Eisenhoffer GT, Gurley KA, Rink JC, Miller DE, Sánchez Alvarado A. Formaldehyde-based whole-mount in situ hybridization method for planarians. Dev Dyn 238(2):443-50, 2009. PMID: 19161223.
- Eisenhoffer GT, Kang H, Sánchez Alvarado A. Molecular analysis of stem cells and their descendants during cell turnover and regeneration in the planarian Schmidtea mediterranea. Cell Stem Cell 3(3):327-39, 2008. PMID: 18786419.
- Adey NB, Lei M, Howard MT, Jensen JD, Mayo DA, Butel DL, Coffin SC, Moyer TC, Slade DE, Spute MK, Hancock AM, Eisenhoffer GT, Dalley BK, McNeely MR. Gains in sensitivity with a device that mixes microarray hybridization solution in a 25-microm-thick chamber. Anal Chem 74(24):6413-7, 2002. PMID: 12510768.