About Dr. Arur
Dr. Arur obtained her Ph.D. with Prof. M.K. Bhan from the All India Institute of Medical Sciences. Dr. Arur undertook post-graduate studies with Dr. David Han, at the UCONN Health Science Center where she spearheaded one of the earliest efforts in deploying state-of-the-art differential proteomics technology (LC-MS/MS coupled with ICAT) to understand how dying cells were cleared by phagocytes. Her studies led to the discovery of the first ‘eat-me’ signal (Annexin I) secreted by the dying apoptotic cell, which enabled its recognition and removal by phagocytes. Subsequently, Dr. Arur received a postdoctoral fellowship from the Washington University School of Medicine in St. Louis to train in the laboratory of Prof. Tim Schedl. During her postdoctoral training, Dr. Arur developed an approach that integrated bioinformatics, proteomics with the power of model-organism genetics to identify substrates of ERK – the terminal kinase of the RAS oncogenic pathway her studies discovered more than 30 new ERK targets and unraveled several new themes in RAS-ERK regulation. Dr. Arur has run her research lab at the MD Anderson Cancer Center since 2010. Her lab focusses on understanding the link between maternal nutrition, insulin like signaling and RAS-ERK pathway, in regulating germ cell development and progression to embryogenesis. In this context, they focus on understanding the function of each ERK substrate during normal organismal development. Their current work has led to the identification of Dicer and Drosha as key ERK phosphorylated substrates in worms and mammals; as well as the analysis and generation of various microRNA landscapes in germ cells. More recently Dr. Arur’s lab has started work in understanding the role of phosphorylated Dicer and Drosha in mouse ES cells and mouse knock-in models. These studies will enable fundamental mechanistic understanding of developmental disorders and oncogenesis Dr. Arur is a Research Scholar of the American Cancer Society, Anna Fuller Foundation, and Andrew Sabin Family Foundation. Her laboratory is currently funded by the National Institute of Health, Cancer Prevention and Research Institute of Texas and American Cancer Society.
View a complete list of Dr. Arur's publications .
Visit Dr. Arur's Lab Website.
Associate Professor, Department of Genetics, Division of Basic Science Research, The University of Texas MD Anderson Cancer Center, Houston, TX
Associate Professor, The University of Texas Graduate School of Biomedical Sciences, Houston, TX
Associate Professor, Division of Basic Science Research, Baylor College of Medicine, Houston, TX
RAS/ERK signaling pathways governing germ cell development ERK-substrate networks Evolution of developmental pathways C. elegans genetics
Germ cells are immortal. They are uniquely poised to sustain life across generations through the fusion of oocyte and sperm. Despite the central importance of germ cells to life, a clear understanding of the molecular events that control their formation is lacking. Germ cells are set aside from somatic cells in the embryo and go through specialized meiotic cell cycles as the animal matures. These cell cycles are interspersed with long periods of arrest. In human females, meiosis I is initiated in the fetus. At birth, oocytes are arrested in meiosis I; after puberty, every month an oocyte initiates meiosis II – ovulation. Upon sperm availability these cells are fertilized, generate an embryo, and the cycle-of-life continues. During meiotic I progression and arrest, the fitness of oocytes and their progeny are influenced by environmental cues and signaling pathways. Yet, little is known about how these factors influence oocyte development and embryo fitness. Using Caenorhabditis elegans, we study the influence of the RAS/ERK pathway on female meiosis I progression. Our research focuses on understanding how the RAS/ERK pathway acts through a battery of substrates to direct multiple cell biological events during meiosis I; how environmental cues impact the RAS/ERK pathway and meiotic progression.
Our laboratory is interested in understanding the molecular and genetic factors that dictate the fitness of the maternal gametes. Specifically, we focus on identifying the function of proteins that are phosphorylated and regulated by environmental signaling and the RAS/ERK pathway to execute different response, and the impact of each response on gamete fitness.
We have identified over thirty different ERK target proteins that function during germ cell development in C. elegans. Currently, we approach each ERK target one at a time, and using a combination of transgenic methods, phosphorylated antibodies, Crispr mediated gene edits and powerful imaging techniques, we are beginning to delineate the role of individual ERK target proteins in differing events of meiotic progression, oocyte development and embryo fitness.
Given the conserved events of meiotic progression, and the RAS/ERK signaling pathway, our research holds the promise of not only uncovering the molecular control of meiosis I at unparalleled resolution to enable better understanding of factors that dictate maternal health and thus progeny survival, but also is poised to uncover novel ERK targets that are regulated during oncogenesis.View a complete list of Dr. Arur's publications .
Visit Dr. Arur's Lab Website.
|2002||All India Institute for Medical Sciences, New Delhi, IND, PHD, Microbiology|
|2007-2010||Instructor, Genetics, Developmental Biology and Cell Biology, Washington University School of Medicine, Saint Louis, MO|
|2002-2007||Post Doctoral Associate, Department of Genetics, Washington University in St Louis, School of Medicine, St. Louis, MO|
|2017||Andrew Sabin Family Foundation Scholar, Andrew Sabin Family Foundation|
|2017||2017 MD Anderson Distinguished Research Faculty Mentor Award, MD Anderson Cancer Center|
|2017||Faculty Educator of the Month, MD Anderson Cancer Center|
|2016||Anna Fuller Foundation Scholar, Anna Fuller Foundation|
|2016||President's Scholar, UT MD Anderson Cancer Center|
|2014||Research Scholar, American Cancer Society|
|2013||Outstanding Faculty Member, Genes and Development, Genes and Development Program, UT MD Anderson Cancer Center|
|1997||Council of Scientific and Industrial Research, Government of India|
- Arur S. Signaling-Mediated Regulation of Meiotic Prophase I and Transition During Oogenesis. Results Probl Cell Differ 59:101-123, 2017. PMID: 28247047.
- Gervaise AL, Arur S. Spatial and Temporal Analysis of Active ERK in the C. elegans Germline. J Vis Exp(117), 2016. e-Pub 2016. PMID: 27929466.
- Mattingly HH, Chen JJ, Arur S, Shvartsman SY. A Transport Model for Estimating the Time Course of ERK Activation in the C. elegans Germline. Biophys J 109(11):2436-45, 2015. PMID: 26636953.
- Drake M, Furuta T, Suen KM, Gonzalez G, Liu B, Kalia A, Ladbury JE, Fire AZ, Skeath JB, Arur S. A Requirement for ERK-Dependent Dicer Phosphorylation in Coordinating Oocyte-to-Embryo Transition in C. elegans. Dev Cell 31(5):614-628, 2014. PMID: 25490268.
- Arur S, Schedl T. Generation and purification of highly specific antibodies for detecting post-translationally modified proteins in vivo. Nat Protoc 9(2):375-95, 2014. e-Pub 2014. PMID: 24457330.
- Putty K, Marcus SA, Mittl PR, Bogadi LE, Hunter AM, Arur S, Berg DE, Sethu P, Kalia A. Robustness of Helicobacter pylori Infection Conferred by Context-Variable Redundancy among Cysteine-Rich Paralogs. PLoS One 8(3):e59560, 2013. e-Pub 2013. PMID: 23555707.
- Yokoo R, Zawadzki KA, Nabeshima K, Drake M, Arur S, Villeneuve AM. COSA-1 Reveals Robust Homeostasis and Separable Licensing and Reinforcement Steps Governing Meiotic Crossovers. Cell 149(1):75-87, 2012. PMID: 22464324.
- Arur S, Ohmachi M, Berkseth M, Nayak S, Hansen D, Zarkower D, Schedl T. MPK-1 ERK Controls Membrane Organization in C. elegans Oogenesis via a Sex-Determination Module. Dev Cell 20(5):677-88, 2011. PMID: 21571224.
- Green RA, Kao HL, Audhya A, Arur S, Mayers JR, Fridolfsson HN, Schulman M, Schloissnig S, Niessen S, Laband K, Wang S, Starr DA, Hyman AA, Schedl T, Desai A, Piano F, Gunsalus KC, Oegema K. A High-Resolution C. elegans Essential Gene Network Based on Phenotypic Profiling of a Complex Tissue. Cell 145(3):470-82, 2011. PMID: 21529718.
- Howell K, Arur S, Schedl T, Sundaram MV. EOR-2 is an Obligate Binding Partner of the BTB-zinc Finger Protein EOR-1 in Caenorhabditis elegans. Genetics 184(4):899-913, 2010. e-Pub 2010. PMID: 20065070.
- Hadwiger G, Dour S, Arur S, Fox P, Nonet ML. A monoclonal antibody toolkit for C. elegans. PLoS One 5(4):e10161, 2010. e-Pub 2010. PMID: 20405020.
- Arur S, Ohmachi M, Nayak S, Hayes M, Miranda A, Hay A, Golden A, Schedl T. Multiple ERK substrates execute single biological processes in Caenorhabditis elegans germ-line development. Proc Natl Acad Sci U S A 106(12):4776-81, 2009. e-Pub 2009. PMID: 19264959.
- Lee MH, Ohmachi M, Arur S, Nayak S, Francis R, Church D, Lambie E, Schedl T. Multiple functions and dynamic activation of MPK-1 extracellular signal-regulated kinase signaling in Caenorhabditis elegans germline development. Genetics 177(4):2039-62, 2007. PMID: 18073423.
- Arur S, Uche UE, Rezaul K, Fong M, Scranton V, Cowan AE, Mohler W, Han DK. Annexin I is an endogenous ligand that mediates apoptotic cell engulfment. Dev Cell 4(4):587-98, 2003. PMID: 12689596.
- Das D, Arur S. Conserved insulin signaling in the regulation of oocyte growth, development, and maturation. Mol Reprod Dev. e-Pub 2017. PMID: 28379636.
- Burton NO, Furuta T, Webster AK, Kaplan RE, Baugh LR, Arur S, Horvitz HR. Insulin-like signalling to the maternal germline controls progeny response to osmotic stress. Nat Cell Biol. e-Pub 2017. PMID: 28166192.
- Snee MJ, Wilson WC, Zhu Y, Chen SY, Wilson BA, Kseib C, O'Neal J, Mahajan N, Tomasson MH, Arur S, Skeath JB. Collaborative Control of Cell Cycle Progression by the RNA Exonuclease Dis3 and Ras is Conserved Across Species. Genetics. e-Pub 2016. PMID: 27029730.
- McCallum KC, Liu B, Fierro-González JC, Swoboda P, Arur S, Miranda-Vizuete A, Garsin DA. TRX-1 Regulates SKN-1 Nuclear Localization Cell Non-autonomously in Caenorhabditis elegans. Genetics. e-Pub 2016. PMID: 26920757.
- Lopez AL, Chen J, Joo HJ, Drake M, Shidate M, Kseib C, Arur S. DAF-2 and ERK Couple Nutrient Availability to Meiotic Progression during Caenorhabditis elegans Oogenesis. Dev Cell. e-Pub 2013. PMID: 24120884.
- Berkseth M, Ikegami K, Arur S, Lieb JD, Zarkower D. TRA-1 ChIP-seq reveals regulators of sexual differentiation and multilevel feedback in nematode sex determination. Proc Natl Acad Sci U S A. e-Pub 2013. PMID: 24046365.
- Suen KM, Lin CC, George R, Melo FA, Biggs ER, Ahmed Z, Drake MN, Arur S, Arold ST, Ladbury JE. Interaction with Shc prevents aberrant Erk activation in the absence of extracellular stimuli. Nat Struct Mol Biol. e-Pub 2013. PMID: 23584453.
- Chen JJ, Arur S. Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline Development. Methods Mol Biol 1487:317-335, 2017. PMID: 27924578.
- Arur S. Context-dependent regulation of Dicer activity and small RNA production: Implications to oocyte-to-embryo transition. Worm 4(4):e1086062, 2015. e-Pub 2015. PMID: 27123367.
Books (edited and written)
- Arur, S. Signaling-Mediated Control of Germ Cells- From Oogenesis to Oocyte-to-Embryo Development. In: Results in Problems and Differentiation. Ed(s) Swathi Arur. Spinger, 2017.
|Title:||Understanding RAS/ERK pathway regulation of small RNA landscape|
|Funding Source:||Andrew Sabin Family Fellows Program|
|Title:||ERK mediated regulation of Dicer and Drosha in C. elegans|